A diagnostic polymerase chain reaction for<i>Mycoplasma iowae</i>using primers located in the intergenic spacer region and the 23S rRNA gene

Ramírez, Ana S.; Dare, C. M.; Yavari, Christine A.; Bradbury, Janet M.

doi:10.1080/03079457.2012.686660

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…Detection limits have been improved from 100 colony forming units (CFU) per PCR reaction (equivalent to 1000 CFU obtained per tracheal swab) using improved amplification products to 32 CFU per PCR reaction (Garcia et al, 1997). This has also been documented on field samples with recent work focussed on improving the specificity (Ramirez et al, 2012). The recent development and field validation of PCR was able to achieve a published diagnostic sensitivity and specificity of 97.6% and 95.5% respectively, with a detection limit of 80 CFU per swab, or 32 CFU per reaction, making PCR a viable diagnostic alternative to culture (Cai et al, 2008).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Mycoplasma iowae in turkeys (Meleagris gallopavo)

Wood¹,

Wilson

2013

World's Poultry Science Journal

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RESUMEN El objetivo fue evaluar algunas características morfológicas de pavos de traspatio (n=248) provenientes de 126 unidades rurales de producción localizadas en 75 municipios de 24 estados de la República Mexicana. El modelo estadístico incluyó sexo, estado y municipio anidado en estado. Las tres variables explicativas afectaron a todas las variables de respuesta (P<0.01), excepto municipio, que no afectó circunferencia de la pechuga (P>0.05). Los machos tuvieron mayor (P<0.001) longitud corporal (10.4 cm más), envergadura (11.4 cm más), circunferencia de la pechuga (13.8 cm más), longitud de tarso (2.5 cm más), peso corporal (2.5 kg más), robustez (9.0 puntos porcentuales más) y solidez (2.8 puntos porcentuales más) que las hembras. Peso corporal mostró estar altamente correlacionado fenotípicamente (P<0.01) con circunferencia de la pechuga tanto en machos (r=0.74) como en hembras (r=0.71). En machos, longitud corporal mostró una correlación baja con longitud del tarso (r=0.25; P<0.01), pero en hembras no estuvo correlacionada (r=0.05; P>0.05). El peso corporal aumentó 143 g (P<0.01) en machos y 113 g en hembras (P<0.01) por cada centímetro que aumentó la circunferencia de la pechuga. Los colores predominantes en el plumaje, la piel y el tarso fueron negro, blanco y café, respectivamente. El pavo de traspatio mexicano presentó dimorfismo sexual significativo y alta correlación fenotípica entre circunferencia de la pechuga y peso corporal. ABSTRACT The objective was to evaluate some morphological characteristics of backyard turkeys (n=248) coming from 126 rural production units located in 75 municipalities of 24 States of the Mexican Republic. The statistical model included sex, state, and municipality within state. The three explanatory variables affected all the response variables (P<0.01), except municipality, which did not affect breast circumference (P>0.05). Male turkeys had greater (P<0.001) body length (10.7 cm more), wingspan (11.4 cm more), breast circumference (13.8 cm more), shank length (2.5 cm more), body weight (2.5 kg more), stockiness (9.0 percentage units more) and massiveness (2.8 percentage units more) than female turkeys. Body weight showed to be highly correlated phenotypically (P<0.01) with breast circumference in both males (r=0.74) and females (r=0.71). Body length was lowly correlated with shank length (r=0.25; P<0.01) in males, but it was not correlated with shank length in females (r=0.05; P>0.05). Body weight increased 143 g in males (P<0.01) and 113 g in females (P<0.01) for each centimeter increment in breast circumference. The predominant colors in the plumage, skin and tarsus were black, white and brown, respectively. The Mexican backyard turkey presented significant sexual dimorphism and strong phenotypic correlation between breast circumference and body weight.

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Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%