A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue

Rizzuto, Emanuele; Carosio, Silvia; Faraldi, Martina; Pisu, Simona; Musarò, Antonio; Prete, Zaccaria Del

doi:10.1155/2016/7465095

Cited by 9 publications

(10 citation statements)

References 27 publications

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“…The results indicated relative errors of less than ~2%. In another work by the same authors 9 , contractility of engineered skeletal muscle tissues was studied with a single high speed camera DIC setup (2D DIC) with acquisition speeds as high as 500 fps. Here, the errors were even lower (~0.15%), showing the great potential of DIC in monitoring the engineered tissue during the process of maturation and growth.…”

Section: Introductionmentioning

confidence: 99%

An Optical Method for the In-Vivo Characterization of the Biomechanical Response of the Right Ventricle

Soltani

Lahti

Järvelä

et al. 2018

Sci Rep

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The intraoperative in-vivo mechanical function of the left ventricle has been studied thoroughly using echocardiography in the past. However, due to technical and anatomical issues, the ultrasound technology cannot easily be focused on the right side of the heart during open-heart surgery, and the function of the right ventricle during the intervention remains largely unexplored. We used optical imaging and digital image correlation for the characterization of the right ventricle motion and deformation during open-heart surgery. This work is a pilot study focusing on one patient only with the aim of establishing the framework for long term research. These experiments show that optical imaging and the analysis of the images can be used to obtain similar parameters, and partly at higher accuracy, for describing the mechanical functioning of the heart as the ultrasound technology. This work describes the optical imaging based method to characterize the mechanical response of the heart in-vivo, and offers new insight into the mechanical function of the right ventricle.

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Section: Introductionmentioning

confidence: 99%

An Optical Method for the In-Vivo Characterization of the Biomechanical Response of the Right Ventricle

Soltani

Lahti

Järvelä

et al. 2018

Sci Rep

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“…The analysis of the X-MET contraction is based on a high resolution microscope joint with a high efficiency camera [11], able to record up to 400 frame per second of 1020×300 pixels gray scale images Fig. 1.…”

Section: The X-met Systemmentioning

confidence: 99%

“…The X-MET system, the microscope (stereomicroscope NIKON SMZ 800 for tissue magnification up to 6.3X) over the sample plate with high frame rate camera (Vendor Basler Model acA2040-180km, Type of data presented Selected samples, Sensor type CMOS, Sensor diagonal Diagonal 15 mm, Resolution 2048×2048 pixel, Pixel width 5.50 µm, Pixel height 5.50 µm), the muscle filament are in the middle of two platinum electrodes that provide the electrical stimulus by a electrostimulator. One end of the muscle is anchored to a piezoelectric micro force transducer to measure the strength [11].…”

Section: The X-met Systemmentioning

confidence: 99%

“…At the IIT@CLNS Biomechanics Laboratory of Rome the researchers have tested a system based on Digital Image Correlation (DIC) [11] for the measurement of small high dynamic biological muscle specimens' contractile properties, that in some cases fails, especially on X-MET muscle tissues movements. Our goal, in this field of investigation, is to build ad-hoc reliable methods to track objects during their movements.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of high frame-rate movies by variational methods

Pedone¹,

Carosio²,

Ruocco³

et al. 2019

Math. Appl.

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The object of this study was the recognition of Regions Of Interest(ROIs) in a time series of digital images of two specific laboratory experiments. It concerns the identification of objects in a tissue surface by high-resolution and high-speed ad-hoc systems for morphological dynamic image analysis. The protocols and the algorithms implemented are developed to retrieve biomechanical properties of two different in vitro systems; the solid filament X-MET (eX-vivo Muscle Engineered Tissue) to measure its reaction to a different frequency stimulation, and a planar system of co-culture of skeletal and cardiac muscle cells, where myotubes and cardiomyocytes coexist, to discriminate the interaction between different cell's type, of its spontaneous pulse. The results of the stimulated X-MET from solid culture system are frequency dependent points of the macroscopic muscular strength and its contractile response. The results for the co-culture planar board measure the correlation of the pulsed movements of the different parts of the tissue.

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“…In previous papers [20], [21], we measured several parameters characterizing the X-MET's isometric contractile capability, as the spontaneous contraction, the kinetic properties, and the force-frequency relationship. However, during normal activity skeletal muscle allows for locomotion of the body encountering isotonic contractions, so that the parameter that better describes muscle and muscle-like tissues functionality is the mechanical power [22], and new methods are continuously proposed to improve its measurements [23], [24].…”

Section: Introductionmentioning

confidence: 99%

Measuring the Maximum Power of an $ex~vivo$ Engineered Muscle Tissue With Isovelocity Shortening Technique

Pisu

Cosentino

Apa

et al. 2019

IEEE Trans. Instrum. Meas.

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The final aim of muscle tissue engineering (TE) is to create a new tissue able to restore the functionality of impaired muscles once transplanted in the site of injury. Therefore, functional contractile properties close to that of healthy muscles are desirable to allow for a good compatibility and a proper functional contribution. Since skeletal muscles deal with locomotion during their normal activity, an accurate measurement of ex vivo muscle engineered tissues' isotonic properties is crucial. In this paper, we devised an experimental system to measure the mechanical power generated by an ex vivo muscle engineered tissue, the X-MET, based on the isovelocity contraction technique. The X-MET is developed without the use of any scaffolds, so that its mechanical properties are not affected by endogenous components. Our experiments allowed for delimiting the ranges of shortening and shortening velocity for which the tissue is able to generate and maintain power for the entire stimulation, which is the condition that better reproduces muscle physiological activity. Then, we measured the power generated by the X-MET and fit the experimental results to the Hill's equation usually employed for modeling the force-velocity relationship of skeletal muscles. The use of this model yielded to the measurement of maximum power and maximum shortening velocity. Results revealed that most of the isotonic properties were consistent with that proposed in the literature for slow-twitch muscles; in particular, the X-METs were able to generate a maximum power of 2.08 ± 0.78 W/kg and had a maximum shortening velocity of 1.84 ± 0.57 L 0 /s, on average.

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A DIC Based Technique to Measure the Contraction of a Skeletal Muscle Engineered Tissue

Cited by 9 publications

References 27 publications

An Optical Method for the In-Vivo Characterization of the Biomechanical Response of the Right Ventricle

An Optical Method for the In-Vivo Characterization of the Biomechanical Response of the Right Ventricle

Analysis of high frame-rate movies by variational methods

Measuring the Maximum Power of an $ex~vivo$ Engineered Muscle Tissue With Isovelocity Shortening Technique

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