A digital microfluidic system for serological immunoassays in remote settings

Ng, Alphonsus H. C.; Fobel, Ryan; Fobel, Christian; Lamanna, Julian; Rackus, Darius; Summers, Amber; Dixon, Christopher; Dryden, Michael D. M.; Lam, Charis; Ho, Margaret T.; Mufti, Nooman S.; Lee, Victor; Asri, Mohd Afiq Mohd; Sykes, Edward A.; Chamberlain, M. Dean; Joseph, Rachael; Ope, Maurice; Scobie, Heather M.; Knipes, Alaine; Rota, Paul A.; Marano, Nina; Chege, Paul M.; Njuguna, Mary Wangui; Nzunza, Rosemary; Kisangau, Ngina; Kiogora, John; Karuingi, Michael; Burton, John Wagacha; Borus, Peter; Lam, Eugene; Wheeler, Aaron R.

doi:10.1126/scitranslmed.aar6076

Cited by 137 publications

(105 citation statements)

References 61 publications

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“…In an attempt to overcome the problems associated with immuno-RDTs, a new highly multiplexed microfluidic immunodiagnostic platform has recently been deployed in Kenya, although the device requires laboratory-like conditions and further optimization for its performance to be equivalent to reference tests (35). Multiplexed PCR techniques for NAAT diagnosis of infectious disease in resource-limited environments have also been reported, using lateral flow for end point detection (22).…”

Section: Discussionmentioning

confidence: 99%

Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

et al. 2017

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The diagnosis of infectious diseases is entering a new and interesting phase. Technologies based on paper microfluidics, coupled to developments in isothermal amplification of Nucleic Acids (NAs) raise opportunities for bringing the methods of molecular biology in the field, in a low setting environment. A lot of work has been performed in the domain over the last few years and the landscape of contributions is rich and diverse. Most often, the level of sample preparation differs, along with the sample nature, the amplification and detection methods, and the design of the device, among other features. In this review, we attempt to offer a structured description of the state of the art. The domain is not mature and there exist bottlenecks that hamper the realization of Nucleic Acid Amplification Tests (NAATs) complying with the constraints of the field in low and middle income countries. In this domain however, the pace of progress is impressively fast. This review is written for a broad Lab on a Chip audience.

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Section: Discussionmentioning

confidence: 99%

Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

et al. 2017

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“…3A) (Ng et al, 2018). Inkjet-printed digital microfluidic cartridges were integrated with an instrument to perform ELISA and combined with a portable control system.…”

Section: On-chip Immunoassaymentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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“…With their open-access platform DropBot (Fobel et al 2013), the Wheeler Group demonstrated detection of a number of biomarkers (thyroid stimulating hormone (Choi et al 2013;Ng et al 2012), 17β-estradiol (Ng et al 2012), Rubella IgG (Dixon et al 2016;Fobel et al 2014;Ng et al 2015a) and Rubella IgM (Ng et al 2015a). Recently, the Wheeler Group has demonstrated in-field diagnostics against measles and rubella immunoglobulin G (IgG) in remote northwestern Kenya's population using a fully integrated version of their DMF immunoassay platform (Ng et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…not requiring external hardware and fully automated. Implementing all these features, the platform developed by the Wheeler Group (Ng et al 2018) and the system described here, developed in parallel, based on defence specifications, are arguably by far, the two most advanced, potentially deployable, DMF biodetection systems.…”

Section: Introductionmentioning

confidence: 99%

Fully integrated digital microfluidics platform for automated immunoassay; A versatile tool for rapid, specific detection of a wide range of pathogens

Coudron

McDonnell

Munro

et al. 2019

Biosensors and Bioelectronics

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A digital microfluidic system for serological immunoassays in remote settings

Abstract: Portable digital microfluidic serological immunoassays for measles and rubella were developed and evaluated in a remote setting.

Cited by 137 publications

References 61 publications

Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

Paper microfluidics for nucleic acid amplification testing (NAAT) of infectious diseases

Recent advances in lab-on-a-chip technologies for viral diagnosis

Fully integrated digital microfluidics platform for automated immunoassay; A versatile tool for rapid, specific detection of a wide range of pathogens

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