A DNA‐Based Plasmonic Nanodevice for Cascade Signal Amplification

Abstract

Here, we describe a DNA circuit-aided, origami nanodevice-based plasmonic system, which performs DNA-regulated, cascade amplification of faint chemical/biological signals. In this system, two goldnanorods (GNRs) are co-assembled onto a DNA lockcontaining, tweezer-like DNA origami template. Logic circuits serve as recognition and amplification elements for specific messengers, producing DNA keys for driving conformational changes of the plasmonic nanodevices. In the presence of input signals including nucleic acids, adenosines, chiral tyrosinamides or specific receptors expressed by tumor cells, the plasmonic nanodevices can be activated to perform dynamic structural motions, reporting robust responses via plasmonic circular dichroism (CD) spectral changes. This DNA nanodevice-based system provides a different design to enrich the strategies for constructing synthetic nanomachines, enabling the customized bottom-up nanostructure construction for sensitive biological signaling.

Gold-Nanoparticle-Based Chiral Plasmonic Nanostructures and Their Biomedical Applications

“…In comparison with discrete chiral plasmonic nanostructures, the dynamically tunable nature of plasmonic superstructures facilitates higher detection sensitivities based on PCD signals. Chiral assemblies have been widely explored for the detection of various bio-related substances, such as metal ions of Hg 2+ [ 26 , 86 ], Ag + [ 87 ], Pb 2+ [ 88 ], Zn 2+ [ 89 ], and Cu 2+ [ 89 , 90 ]; DNA/RNA [ 91 , 92 , 93 , 94 , 95 ]; amino acids/peptides [ 96 , 97 , 98 ]; proteins [ 99 , 100 ]; antigens [ 101 , 102 ]; and other biomarker molecules [ 103 , 104 , 105 ].…”

DNA-mediated dynamic plasmonic nanostructures: assembly, actuation, optical properties, and biological applications

“…10e). 175 As the DNA-mediated nanoparticle assembly has been widely used to build complex structures and sensing platforms, the plasmonic CD responses obtained by asymmetric nanoassemblies will enable the creation of intelligent materials with unique optical responses and the fabrication of ultrasensitive sensors.…”

Biomolecule‐Based Circularly Polarized Luminescent Materials: Construction, Progress, and Applications

“…Ding et al described an origami nanodevice plasma system based on DNA circuits that produces strong CD signals when interacting with CPL in the presence of input signals, including nucleic acids, adenosine, chiral tyrosine, or specific receptors expressed by tumor cells, and can be cascaded to amplify the signals. [167] Because there is only a slight difference in the number of photons absorbed by biomolecules, the biological effect of CPL on living cells is almost negligible. However, through the assembly of biomolecules and nanoparticles, researchers can improve their polarizability and interact strongly with CPL.…”