A DNA macro-array to simultaneously identify 32 meat species in food samples

Identification of donkey meat in foods using species-specific PCR combined with lateral flow immunoassay

“…involved in the meat authentic issue related to donkey meat. Compared to the scope of the previous studies in which mainly focused on major meats available in common grocery stores in developed countries, 6,7,23,27,28,31,33 our current study also included many species that are poorly monitored but do exist in meat products produced in Africa 9 and Asia (internal data). This provides a more powerful tool for regulatory agencies with high condence in reducing the false positive issue during the routine tests.…”

“…28 Furthermore, a species-specic PCR method was reported to identify pork, horse meat, and donkey meat, achieving a limit of detection (LOD) as low as 0.1% w/w donkey meat in beef. 29 Besides, real-time PCR, 23 multiplex PCR, 30,31 PCR-DNA pyrosequencing, 32 DNA microarray 33 and others have also been reported for species-specic detection of donkey meat. Nevertheless, their methods bear some limitations, such as a limited number of species for specicity test, 29 relatively labour intensive bench work (e.g., electrophoresis), 28 and the use of expensive equipment.…”

Verification of animal species in ham and salami by DNA microarray and Real time PCR methods

“…Verification of genuineness of certain products is a necessary part of a comprehensive examination of quality with regard to consumer protection (Maršálková et al, 2014). Considering the recent cases of meat adulteration and fraud, efficient and accurate analytical methods are essential for identification of meat species as a key importance to maintain consumer trust and to comply with labelling legislations (Cottenet, 2016).…”

“…In this context, wild game meats may originate from farms having regulated hygienic standards and fair commercial practices (Hoffman and Wiklund, 2006;Fajardo et al, 2010). Following the horse meat crisis which spread throughout Europe in 2013, food fraud and adulteration are identified as a top priority addressed by authorities, regulators and food industries (Elliott, 2014, Cottenet et al, 2016. Economically motivated adulteration presents many challenges because perpetrators are specifically seeking to avoid detection and circumvent existing regulatory systems or testing methodologies (Everstine, Spink and Kennedy, 2013; Cottenet et al, 2016).…”

Αξιολόγηση Της Ποιότητα, Ασφάλειας Και Αυθεντικότητας Του Κρέατος Με Ταχείες Μεταβολομικές Μεθόδους

“…Πρόσφατα, η μέθοδος «Meat LCD Array», έχει επιφέρει αποτελεσματική και αξιόπιστη αναγνώριση του κρέατος, επιτρέποντας την ταυτόχρονη ανίχνευση 32 ειδών (Cavin et al, 2016;Cottenet et al, 2016). Τέλος, προτάθηκε η αναγνώριση χοιρινού κρέατος σε ωμά ή μαγειρεμένα κεφτεδάκια εντός 20 λεπτου με συνδυασμό PCR και οπτική ανίχνευση (H. Wu et al, 2020).…”