A double-coated magnetite-based magnetic fluid evaluation by cytometry and genetic tests

Freitas, Marisa; Silva, L.P.; Azevedo, Ricardo Bentes; Garcia, Velia; Lacava, L.M.; Grisólia, César Koppe; Lucci, Carolina Madeira; Morais, P.C.; Silva, M.F. Da; Buske, N.; Curi, R.; Lacava, Z.G.M.

doi:10.1016/s0304-8853(02)00655-8

Cited by 33 publications

(27 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Significant differences were not observed in the most abundant peritoneal cell populations (macrophages and lymphocytes). However, some fluctuations in peritoneal cell populations related to the inflammatory response (neutrophils and eosinophils) 19,[30][31][32] were detected until 7 days, even after just one single injection of MAN, as observed previously. These fluctuations in peritoneal cell viability may be associated with the particulate state of the intraperitoneally administered MNPs and the consequent responses of neutrophil and eosinophil populations.…”

Section: Discussionsupporting

confidence: 83%

“…Although micronuclei development may be associated with reactive oxygen species (ROS) induced by iron excess, 23,33 in the present study, the MN assay showed no MAN effect on cell division or genotoxicity, differently from other samples based on magnetic nanoparticles. 19,20,32 Also, the % PCE indicated that the MAN sample was not cytotoxic to the erythrocytic lineage in bone marrow.…”

Section: Discussionmentioning

confidence: 99%

“…For the differential leukocyte count, 19 blood smears or peritoneal cells imprinted in glass slides were fixed in methanol for 5 minutes, stained with Wright-Giemsa dye, and observed under light microscope (n = 500 cells).…”

Section: Total and Differential Leukocyte Countsmentioning

confidence: 99%

See 2 more Smart Citations

Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

Estevanato

Cintra²,

Baldini³

et al. 2011

IJN

View full text Add to dashboard Cite

Background: The magnetic albumin nanosphere (MAN), encapsulating maghemite nanoparticles, was designed as a magnetic drug delivery system (MDDS) able to perform a variety of biomedical applications. It is noteworthy that MAN was efficient in treating Ehrlich’s tumors by the magnetohyperthermia procedure. Methods and materials: In this study, several nanotoxicity tests were systematically carried out in mice from 30 minutes until 30 days after MAN injection to investigate their biocompatibility status. Cytometry analysis, viability tests, micronucleus assay, and histological analysis were performed. Results: Cytometry analysis and viability tests revealed MAN promotes only slight and temporary alterations in the frequency of both leukocyte populations and viable peritoneal cells, respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, interestingly, revealed the presence of MAN clusters in the central nervous system (CNS). Conclusion: The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

Estevanato

Cintra²,

Baldini³

et al. 2011

IJN

View full text Add to dashboard Cite

show abstract

“…Most widely used biocompatible magnetic fluid is magnetite (Fe 3 O 4 ) based [4]. Several surfactant-and polymer-based coating materials like citrate, polyaspertic acid, dextran have been tested for biocompatibility [4][5][6][7][8]. Dextran coating has been used mostly because of its biocompatibility [4,9].…”

mentioning

confidence: 99%

“…Iron deposition within the cells was detected by characteristic blue color of prussian blue. For this study, cells were first grown on cover slips for 24 h after which they were exposed to LMN and DMN at different concentrations (0.2 and 0.4 mg/ml of cell culture suspension) for 24 and 48 h. The cover slips were then immersed in a mixture containing equal volume of 20% aqueous solution of HCl and 10% aqueous solution of potassium ferrocyanide [K 4 Fe(CN) 6 , 3H 2 O] for 20 min and counterstaining was done by nuclear fast red for 5 min. Photographs were taken in bright field mode using Olympus BX 51 microscope.…”

mentioning

confidence: 99%