Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

Abstract

Background: The magnetic albumin nanosphere (MAN), encapsulating maghemite nanoparticles, was designed as a magnetic drug delivery system (MDDS) able to perform a variety of biomedical applications. It is noteworthy that MAN was efficient in treating Ehrlich’s tumors by the magnetohyperthermia procedure. Methods and materials: In this study, several nanotoxicity tests were systematically carried out in mice from 30 minutes until 30 days after MAN injection to investigate their biocompatibility status. Cytometry analysis, viability tests, micronucleus assay, and histological analysis were performed. Results: Cytometry analysis and viability tests revealed MAN promotes only slight and temporary alterations in the frequency of both leukocyte populations and viable peritoneal cells, respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, interestingly, revealed the presence of MAN clusters in the central nervous system (CNS). Conclusion: The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.

<p>The influence of female mice age on biodistribution and biocompatibility of citrate-coated magnetic nanoparticles</p>

“…Accordingly, young mice exposed to a bolus dose of dextran-coated magnetite NPs did not show morphological or ultramicroscopic alterations, even when the MNPs were retained for long term (6 months) in the liver and spleen. 50 It is well established that excessive iron, such as iron coming from metabolized NPs, is gradually deposited in various organs, 51,52 especially in the spleen and liver, 32,53,54 corroborating the findings of the present study in both elderly and young animals. However, some differences related to age were found.…”

The pequi pulp oil (Caryocar brasiliense Camb.) provides protection against aging-related anemia, inflammation and oxidative stress in Swiss mice, especially in females

“…After euthanasia, bone marrow cells were surgically removed to carry out the micronucleus (MN) test according to a standard method ( Schmid, 1975 ) with modifications ( Estevanato et al , 2011 ). Briefly, the bone marrow cells were collected with 1 mL of fetal bovine serum (FBS) and centrifuged at 160 x g for 5 min.…”

Síntese de modelos esferoidais de células melanômicas em diferentes graus de progressão associados a sistemas dermo-equivalentes para tratamento via terapia fotodinâmica

“…Diversas estratégias de encapsulação vêm sendo desenvolvidas e utilizadas nos últimos anos para aumentar a solubilidade dos ativos, assim como a biodisponibilidade de compostos lipofílicos. O desenvolvimento de sistemas de "drug delivery", ou sistemas veiculadores de fármacos, com ênfase especial no design e fabricação de sistemas para tratamento de câncer são elaborados com o intuito de minimizar a degradação do fármaco após a administração, prevenir efeitos colaterais indesejáveis e sustentar e/ou aumentar a biodisponibilidade do medicamento na área alvo (Estevanato et al, 2011;Siqueira-Moura et al, 2013). Também mencionados como carreadores, esses sistemas são projetados para melhorar a solubilidade em água e a estabilidade química dos agentes ativos carreados, assim como aumentar a atividade farmacológica e reduzir os efeitos colaterais.…”