A dried blood spots technique based LC–MS/MS method for the analysis of posaconazole in human whole blood samples

Managing The Effect of Hematocrit on Dbs Analysis in A Regulated Environment

“…Assessing the effect of hematocrit over a range of normal hematocrit values for the matrix of interest [2] during method development and validation is common practice [4]. The typical acceptance criterion is a bias of <15% for quality control samples within the tested hematocrit range.…”

A simple dried blood spot method for therapeutic drug monitoring of the tricyclic antidepressants amitriptyline, nortriptyline, imipramine, clomipramine, and their active metabolites using LC-MS/MS

“…Over the last decade, a rising number of DBS assays for different classes of drugs have been reported. So far, antidiabetics, immunosuppressants, analgesics, anti-HIV drugs, antihypertensive drugs, antimicrobial agents, anti-epileptics, and the iron chelator deferasirox have been included [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Among the antidepressants, a DBS assay for analysis of IMP has previously been described as well as for the SSRIs fluoxetine, reboxetine, and paroxetine, and for the atypical antidepressant venlafaxine [26][27][28].…”

Procedures and Practices for The Validation of Bioanalytical Methods Using Dried Blood Spots: A Review

“…The majority of these reports describe no effect of spot volume on assay performance. The few articles that reported an effect of spot volume; a low spot volume resulted in a lower analyzed concentration, attributed this effect to the fact that the obtained DBS was too small for the punching device that was used [18,59,86,87].…”

“…Other reports describe the use of a smaller punch from the DBS sample. The actual concentration of these samples is calculated using the area of the punches [50,[59][60]. One of these reports takes the decreased amount of matrix into account by using the 'doughnut punch method'.…”