Managing The Effect of Hematocrit on Dbs Analysis in A Regulated Environment

Fan, Leimin; Lee, Jacob A

doi:10.4155/bio.11.337

Cited by 78 publications

(52 citation statements)

References 6 publications

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“…This is in keeping with the findings of other studies, such as neonatal screening programs, where all spots are considered equal, therefore giving equivalent analyte recovery results when using the same-sized punch (11,28,29).…”

Section: Discussionsupporting

confidence: 71%

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Duncombe

Commander

Erdenliğ

et al. 2013

Clin Vaccine Immunol

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c Brucella abortus, a smooth strain of the genus Brucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples from Brucella-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-Brucella smooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-␥) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-␥ ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-␥. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-␥ recovery over 10 days when stored at room temperature.

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Section: Discussionsupporting

confidence: 71%

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Duncombe

Commander

Erdenliğ

et al. 2013

Clin Vaccine Immunol

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“…Youhnovski et al published a similar concept named pre-cut dried blood spot (PCDBS) and reported good analytical performance and haematocrit independency (85) . Likewise Fan and Lee reported a similar commercially available approach named dried matrix in paper disc (DMPD) (86) .…”

Section: Haematocritmentioning

confidence: 99%

Direct Analysis of Dried Blood Spot Samples

Abu-Rabie¹

2014

Dried Blood Spots

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“…), hematocrit impact on quantitative analysis of DBS samples are minimal. Furthermore, strategies aimed at consistently collecting and analyzing a precise volume of blood to mitigate this impact have been evolving and appear to be quite promising (29)(30)(31).…”

Section: Practical Bioanalytical Considerations For Implementing Dbsmentioning

confidence: 99%

Implementing Dried Blood Spot Sampling for Clinical Pharmacokinetic Determinations: Considerations from the IQ Consortium Microsampling Working Group

Evans

Arnold

Bryan

et al. 2014

AAPS J

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Managing The Effect of Hematocrit on Dbs Analysis in A Regulated Environment

Cited by 78 publications

References 6 publications

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

Direct Analysis of Dried Blood Spot Samples

Implementing Dried Blood Spot Sampling for Clinical Pharmacokinetic Determinations: Considerations from the IQ Consortium Microsampling Working Group

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