A Drug‐Free, Hair Follicle Cycling Regulatable, Separable, Antibacterial Microneedle Patch for Hair Regeneration Therapy

Abstract

The development of painless hair loss therapy without side-effect is challenging. The dermal papilla is the signal center of hair follicles and plays a key role in the regulation of their cycling. Activation of dermal papilla cells (DPCs) would promote hair regeneration. In this study, a separable microneedle patch comprised of chitosan lactate (CL) and exosomes (EXO) from adipose-derived stem cells is fabricated. After insertion of the microneedle into the skin, the hyaluronic acid substrate dissolves fast and the swellable polyvinyl alcohol needles are retained. The EXO sustainedly released from needles can be endocytosed by DPCs and promote cell proliferation via the activation of the Wnt signaling pathway, while the L-lactate released by CL can promote cell growth by activating lactate dehydrogenase. CL and EXO synergetically facilitate hair regeneration through regulating hair follicle cycling. In animal tests, compared with topical administration of minoxidil, the drug-free microneedle patches can more significantly promote hair regeneration within 7 days with lower dosing frequency. Furthermore, the inherent antibacterial properties of CL make it possible to avoid potential infection. Such transdermally administrated drug-free microneedle patches provide a simple, safe, and efficient strategy for hair loss treatment and exhibit great potential in clinical application.

Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

“…Because MN systems allow for deeper drug delivery and the ability to design structures with multiple functions, several research teams have designed EV-loaded hydrogel MN patches for hair regeneration [ 237 , 238 ]. Yang et al.…”

“…Alternatively, Shi et al. [ 238 ] prepared detachable MN patches consisting of chitosan lactate (CL) and ADSC-Exos for hair regeneration. After insertion of the MN into the skin, the HA substrate was rapidly dissolved and the expandable polyvinyl alcohol tip was retained.…”

“… [ 139 ] CL microneedle ADSC Microneedle Such transdermally administrated microneedle patches provide a simple, safe and efficient strategy for hair regeneration. [ 238 ] Liver PEG MSC Intraperitoneal injection EV-loaded hydrogel based on click reaction has potential for systemic drug delivery. [ 148 ] Abbreviations: PF-127: Pluronic F-127; ADSC: Adipose derived mesenchymal stem cells; MMP: matrix metalloproteinase; PEG: Polyethylene glycol; HAMA: Hyaluronic acid Methacryloyl; BMSC: Bone marrow mesenchymal stem cells; MSC: mesenchymal stem cells; HA: Hyaluronic Acid; MC-CHO: Aldehyde methylcellulose; CS-g-PEG: chitosan-g-PEG; GelMA: Gelatin methacryloyl; LAP: Lithium Phenyl (2,4,6-trimethylbenzoyl) phosphinate; β-TCP: β-Tricalcium Phosphate; PLGA: poly (lactic- co -glycolic acid; PLLA: poly( l -lactic acid); nHP: nanohydroxyapatite/poly-ε-caprolactone; CGC: chitosan-gelatin-chondroitin sulfate; HG: nano-hydroxyapatite-gelatin; AD/CS/RSF: alginate-dopamine, chondroitin sulfate, and regenerated silk fibroin; UMSC: umbilical cord mesenchymal stem cells; ECM: extracellular matrix; iPS-CPCs: pluripotent stem cell-derived cardiac progenitors; PDNPs-PELA: Polydopamine nanoparticles incorporated poly (ethylene glycol)-poly(ε-cap-rolactone- co -lactide); OSA: oxidized sodium alginate; CL: chitosan lactate.…”

Application of lncRNA–miRNA–mRNA ceRNA network analysis in the treatment of androgenic alopecia

“…In addition, some new treatment methods have emerged, such as low-intensity laser irradiation, 4 autologous platelet-rich plasmas injections, 5 and microneedle therapy. 6 However, these methods have not been shown to be superior to the existing standard therapies for hair loss, and further clinical trials are needed to verify their efficacy.…”

“…Scientifically proven treatments for AGA include oral finasteride and topical minoxidil, both of which have been approved by the US Food and Drug Administration. In addition, some new treatment methods have emerged, such as low‐intensity laser irradiation, 4 autologous platelet‐rich plasmas injections, 5 and microneedle therapy 6 . However, these methods have not been shown to be superior to the existing standard therapies for hair loss, and further clinical trials are needed to verify their efficacy.…”