A drug-loaded composite coating to improve osteogenic and antibacterial properties of Zn–1Mg porous scaffolds as biodegradable bone implants

Zhang, Zhenbao; Liu, Aobo; Fan, Jing; Wang, Menglin; Dai, Jiabao; Jin, Xiang; Deng, Huanze; Wang, Xuan; Liang, Yijie; Li, Haixia; Zhao, Yantao; Wen, Peng; Li, Yanfeng

doi:10.1016/j.bioactmat.2023.04.017

Cited by 13 publications

(15 citation statements)

References 83 publications

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“…3 Reportedly, the ion concentration of less than 100 μg/mL for Mg 2+ has no cytotoxic effect on the MC3T3-E1 cells for Zn-1Mg alloy. 4 The researcher observed different tolerance levels of the ion concentration (Cu 2+ and Mg 2+ ) for different cells. 49 Therefore, the presence of low concentrations of Cu 2+ and Mg 2+ in the solution confirms that the cytocompatibility of the present alloys is mainly affected by the Zn 2+ ion.…”

Section: Cytotoxicity Studymentioning

confidence: 99%

“…After 5 days of culture, ZHN2 illustrates higher cell viability of ∼117%, which may be related to the nanoscale roughness, higher hydrophilicity, and lower degradation rate of the samples, promoting higher protein adsorption and ultimately increasing cell growth. 4,50 Prabhu et al have also reported that the addition of neem in bioactive glass increases the in vitro biocompatibility of the human gastric adenocarcinoma cell line (AGS). 51 Moreover, It is also important to mention that the addition of brushite/HAp coating over the Zn−Cu alloy substrate significantly improves the osteoconductivity due to the regulation of the concentration-dependent phenomena of Zn 2+ ions, and the presence of Ca and P, which have a significant role in bone mineralization phenomena.…”

Section: Cytotoxicity Studymentioning

confidence: 99%

“…In addition, the presence of Ca 2+ ions in the coating provides the nucleation sites for apatite formation, which further encourages osteoblastic proliferation, growth, and mineralization. 4 In vivo biosafety response of the implanted scaffolds is evaluated through the H/E staining of the important organs (heart, kidney, liver, and spleen), as shown in Figure 14. There are no observable abnormalities, accumulation of the degradation products, or any histopathological changes exhibiting favorable in vivo biosafety.…”

Section: Cytotoxicity Studymentioning

confidence: 99%

“…3 Although traditional biomedical metals like titanium (Ti) and stainless steel provide stable mechanical support for long-term clinical applications, their existence as biodegradable implants is limited due to their improper degradation rate and long-term stress shielding effect. 4 Another point of concern is the facilitation of bacterial colonization and infection after the implantation of the implants, which limits the application of permanent implants. The chances of infection can persist up to 6 months after implant removal.…”

Section: Introductionmentioning

confidence: 99%

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Feasibility Insights of the Green-Assisted Calcium-Phosphate Coating on Biodegradable Zinc Alloys for Biomedical Application: In Vitro and In Vivo Studies

Palai,

De,

Prasad

et al. 2024

ACS Appl. Mater. Interfaces

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In the field of bone tissue engineering, recently developed Zn alloy scaffolds are considered potential candidates for biodegradable implants for bone regeneration and defect reconstruction. However, the clinical success of these alloys is limited due to their insufficient surface bioactivities. Further, the higher concentration of Zn 2+ produced during degradation promotes antibacterial activity, but deteriorates osteogenic properties. This study fabricated an Azadirachta indica (neem)-assisted brushite-hydroxyapatite (HAp) coating on the recently developed Zn-2Cu-0.5Mg alloy to tackle the above dilemma. The microstructure, degradation behavior, antibacterial activity, and hemocompatibility, along with in vitro and in vivo cytocompatibility of the coated alloys, are systematically investigated. Microstructural analysis reveals flower-like morphology with uniformly grown flakes for neem-assisted deposition. The neem-assisted deposition significantly improves the adhesion strength from 12.7 to 18.8 MPa, enhancing the mechanical integrity. The potentiodynamic polarization study shows that the neem-assisted deposition decreases the degradation rate, with the lowest degradation rate of 0.027 mm/yr for the ZHN2 sample. In addition, the biomineralization process shows the apatite formation on the deposited coating after 21 days of immersion. In vitro cytotoxicity assay exhibits the maximum cell viability of 117% for neem-assisted coated alloy in 30% extract after 5d and the improved cytocompatibility which is due to the controlled release of Zn 2+ ions. Meanwhile, neem-assisted coated alloy increases the ZOI by 32 and 24% for Gram-positive and Gram-negative bacteria, respectively. Acceptable hemolysis (<5%) and anticoagulation parameters demonstrate a promising hemocompatibility of the coated alloy. In vivo implantation illustrates a slight inflammatory response and vascularization after 2 weeks of subcutaneous implantation, and neo-bone formation in the defect areas of the rat femur. Micro-CT and histology studies demonstrate better osseointegration with satisfactory biosafety response for the neem-assisted coated alloy as compared to that without neem-assisted deposition. Hence, this neem-assisted brushite-Hap coating strategy elucidates a new perspective on the surface modification of biodegradable implants for the treatment of bone defects.

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Section: Cytotoxicity Studymentioning

confidence: 99%

Section: Cytotoxicity Studymentioning

confidence: 99%

Section: Cytotoxicity Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Feasibility Insights of the Green-Assisted Calcium-Phosphate Coating on Biodegradable Zinc Alloys for Biomedical Application: In Vitro and In Vivo Studies

Palai,

De,

Prasad

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…In addition to design according to the bacterial microenvironment, controlled release of functional ions is commonly used to maintain a balance between antibacterial and osteogenic abilities. , The release of ions from implants plays a crucial role in regulating the behavior of bacteria and cells, as these ions can significantly impact their survival and metabolism . However, achieving effective bacterial clearance with small doses of functional ions is challenging.…”

Section: Balance the Antibacterial And Osteogenic Capabilitiesmentioning

confidence: 99%

External Stimuli-Responsive Strategies for Surface Modification of Orthopedic Implants: Killing Bacteria and Enhancing Osteogenesis

Liu,

Feng,

Ran

et al. 2024

ACS Appl. Mater. Interfaces

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Bacterial infection and insufficient osteogenic activity are the main causes of orthopedic implant failure. Conventional surface modification methods are difficult to meet the requirements for longterm implant placement. In order to better regulate the function of implant surfaces, especially to improve both the antibacterial and osteogenic activity, external stimuli-responsive (ESR) strategies have been employed for the surface modification of orthopedic implants. External stimuli act as "smart switches" to regulate the surface interactions with bacteria and cells. The balance between antibacterial and osteogenic capabilities of implant surfaces can be achieved through these specific ESR manifestations, including temperature changes, reactive oxygen species production, controlled release of bioactive molecules, controlled release of functional ions, etc. This Review summarizes the recent progress on different ESR strategies (based on light, ultrasound, electric, and magnetic fields) that can effectively balance antibacterial performance and osteogenic capability of orthopedic implants. Furthermore, the current limitations and challenges of ESR strategies for surface modification of orthopedic implants as well as future development direction are also discussed.

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Mechanics‐Resilient HA/SIS‐Based Composite Scaffolds with ROS‐Scavenging and Bacteria‐Resistant Capacity to Address Infected Bone Regeneration

Song,

Yu,

Hou

et al. 2024

Adv Funct Materials

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To address and regenerate infected bone defects complicated by issues such as inflammation and bone resorption, and to promote bone regeneration, this study focuses on the development of a composite scaffold with reactive oxygen species (ROS)‐scavenging and bacteria‐resistant properties. The composite scaffold integrates a self‐assembled small intestinal submucosa (SIS) hydrogel with pre‐adsorbed hydroxyapatite (HA) particles and tannic acid (TA), demonstrating distinctive mechanical resilience and porous structures, suitable for filling irregular cavities and facilitating cell infiltration, while exhibiting a broad‐spectrum of antibacterial efficacy and robust ROS‐scavenging capacity for tissue regeneration. RNA‐sequencing analysis indicates the underlying mechanism revealing the disrupting of arginine and alanine amino acid biosynthesis. Furthermore, the composite scaffold demonstrates excellent cytocompatibility, with cell viability exceeding 70%. Remarkably, it demonstrates exceptional anti‐inflammatory performances (≈5‐fold to the control). In an infected bone defect model, the composite scaffold facilitates superior bone regeneration, being ≈5‐fold greater than the control, while maintaining a conducive environment for cell adhesion and infiltration without scaffold collapse. This multifunctional composite scaffold emerges as a promising candidate for combating infections in bone regeneration, showcasing its potential in addressing complex bone‐related challenges.

show abstract

A drug-loaded composite coating to improve osteogenic and antibacterial properties of Zn–1Mg porous scaffolds as biodegradable bone implants

Cited by 13 publications

References 83 publications

Feasibility Insights of the Green-Assisted Calcium-Phosphate Coating on Biodegradable Zinc Alloys for Biomedical Application: In Vitro and In Vivo Studies

Feasibility Insights of the Green-Assisted Calcium-Phosphate Coating on Biodegradable Zinc Alloys for Biomedical Application: In Vitro and In Vivo Studies

External Stimuli-Responsive Strategies for Surface Modification of Orthopedic Implants: Killing Bacteria and Enhancing Osteogenesis

Mechanics‐Resilient HA/SIS‐Based Composite Scaffolds with ROS‐Scavenging and Bacteria‐Resistant Capacity to Address Infected Bone Regeneration

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