A dual function for Pex3p in peroxisome formation and inheritance

Munck, Joanne M.; Motley, Alison M.; Nuttall, James; Hettema, Ewald H.

doi:10.1083/jcb.200906161

Cited by 63 publications

(68 citation statements)

References 35 publications

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“…Basically, de novo formation of peroxisomes from the endoplasmic reticulum seems to be associated with the Pex3 protein, which plays also a role in peroxisome inheritance [86]. This peroxin is suggested to be essential for the formation of initial vesicular compartments termed pre-peroxisomes, which grow by recruiting newly synthesized peroxisomal membrane proteins and matrix proteins to form a mature peroxisome that multiplies by Wssion.…”

Section: Peroxisome Biogenesis and Abundancementioning

confidence: 99%

Role of peroxisomes in the biosynthesis and secretion of β-lactams and other secondary metabolites

Martı́n

Ullán

Garcı́a-Estrada

2012

Journal of Industrial Microbiology and Biotechnology

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Peroxisomes are eukaryotic organelles surrounded by a single bilayer membrane, containing a variety of proteins depending on the organism; they mainly perform degradation reactions of toxic metabolites (detoxification), catabolism of linear and branched-chain fatty acids, and removal of H(2)O(2) (formed in some oxidative processes) by catalase. Proteins named peroxins are involved in recruiting, transporting, and introducing the peroxisomal matrix proteins into the peroxisomes. The matrix proteins contain the peroxisomal targeting signals PTS1 and/or PTS2 that are recognized by the peroxins Pex5 and Pex7, respectively. Initial evidence indicated that the penicillin biosynthetic enzyme isopenicillin N acyltransferase (IAT) of Penicillium chrysogenum is located inside peroxisomes. There is now solid evidence (based on electron microscopy and/or biochemical data) confirming that IAT and the phenylacetic acid- and fatty acid-activating enzymes are also located in peroxisomes. Similarly, the Acremonium chrysogenum CefD1 and CefD2 proteins that perform the central reactions (activation and epimerization of isopenicillin N) of the cephalosporin pathway are targeted to peroxisomes. Growing evidence supports the conclusion that some enzymes involved in the biosynthesis of mycotoxins (e.g., AK-toxin), and the biosynthesis of signaling molecules in plants (e.g., jasmonic acid or auxins) occur in peroxisomes. The high concentration of substrates (in many cases toxic to the cytoplasm) and enzymes inside the peroxisomes allows efficient synthesis of metabolites with interesting biological or pharmacological activities. This compartmentalization poses additional challenges to the cell due to the need to import the substrates into the peroxisomes and to export the final products; the transporters involved in these processes are still very poorly known. This article focuses on new aspects of the metabolic processes occurring in peroxisomes, namely the degradation and detoxification processes that lead to the biosynthesis and secretion of secondary metabolites.

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Section: Peroxisome Biogenesis and Abundancementioning

confidence: 99%

Role of peroxisomes in the biosynthesis and secretion of β-lactams and other secondary metabolites

Martı́n

Ullán

Garcı́a-Estrada

2012

Journal of Industrial Microbiology and Biotechnology

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“…Pex3 regulates peroxisome biogenesis by acting as a docking factor for Pex19, the protein that recognizes the membrane PTS on PMPs and facilitates their post-transla-tional insertion into the peroxisomal membrane (20 -23); as a tether for the peroxisome inheritance factor Inp1 (24,25); as the receptor for Myo2 for peroxisome segregation in Yarrowia lipolytica (26); and as an anchor at the peroxisomal membrane for P. pastoris Atg30 and S. cerevisiae Atg36 (10,16). Additionally, Pex3 has been implicated in pexophagy in other species, such as the methylotrophic yeast Hansenula polymorpha, in which removal of ubiquitinated Pex3 from the peroxisomal membrane induces pexophagy, and in mammals, in which Pex3 overexpression induces pexophagy through a ubiquitin-dependent mechanism (27,28).…”

mentioning

confidence: 99%

Peroxisomal Pex3 Activates Selective Autophagy of Peroxisomes via Interaction with the Pexophagy Receptor Atg30

Burnett

Farré

Nazarko

et al. 2015

Journal of Biological Chemistry

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“…Thus, Inp1p was concluded to be required for attaching peroxisomes to a then uncharacterized cortical anchor (Fagarasanu et al, 2005). The identification of mutants of Pex3p that are unable to recruit Inp1p to the peroxisomal membrane and exhibit the same phenotype as that of inp1D cells (Munck et al, 2009) indicated that there is a direct interaction between Pex3p and Inp1p. Pex3p is essential for peroxisome biogenesis and is integral to both the ER and the peroxisomal membranes (Hoepfner et al, 2005;Thoms et al, 2012).…”

Section: Commentarymentioning

confidence: 99%

Sharing the cell's bounty – organelle inheritance in yeast

Knoblach

Rachubinski

2015

Journal of Cell Science

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Eukaryotic cells replicate and partition their organelles between the mother cell and the daughter cell at cytokinesis. Polarized cells, notably the budding yeast Saccharomyces cerevisiae, are well suited for the study of organelle inheritance, as they facilitate an experimental dissection of organelle transport and retention processes. Much progress has been made in defining the molecular players involved in organelle partitioning in yeast. Each organelle uses a distinct set of factors -motor, anchor and adaptor proteins -that ensures its inheritance by future generations of cells. We propose that all organelles, regardless of origin or copy number, are partitioned by the same fundamental mechanism involving division and segregation. Thus, the mother cell keeps, and the daughter cell receives, their fair and equitable share of organelles. This mechanism of partitioning moreover facilitates the segregation of organelle fragments that are not functionally equivalent. In this Commentary, we describe how this principle of organelle population control affects peroxisomes and other organelles, and outline its implications for yeast life span and rejuvenation.

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A dual function for Pex3p in peroxisome formation and inheritance

Abstract: Pex3p interacts with peroxisome retention factor Inp1p at the peroxisomal membrane and functions in the organelle’s segregation in addition to its biogenesis.

Cited by 63 publications

References 35 publications

Role of peroxisomes in the biosynthesis and secretion of β-lactams and other secondary metabolites

Role of peroxisomes in the biosynthesis and secretion of β-lactams and other secondary metabolites

Peroxisomal Pex3 Activates Selective Autophagy of Peroxisomes via Interaction with the Pexophagy Receptor Atg30

Sharing the cell's bounty – organelle inheritance in yeast

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