A dual-potential electrochemiluminescence ratiometric approach based on graphene quantum dots and luminol for highly sensitive detection of protein kinase activity

Abstract

A novel Au NP mediated dual-potential ECL ratiometric approach for highly sensitive protein kinase activity and inhibition assay has been developed based on the simultaneous decrease of cathodic ECL from GQDs and enhancement of anodic ECL from luminol in the same bioanalysis.

Carbon-based dots for electrochemiluminescence sensing

“…On the other hand, dual luminophores provided an opportunity to design the potential-resolved ECL ratiometric method by using gold nanoparticles as signal transduction units for regulating the cathodic ECL signal of GDs and the anodic ECL signal of luminol. 162 The GDs were first modified on the electrode surface and then conjugated with peptides. In the presence of adenosine 5 0 -[g-thio]triphosphate (ATP-s) and protein kinase, the peptides were phosphorylated to form thiophosphorylated peptides, and then specifically captured gold nanoparticles, which quenched the ECL of GDs owing to RET.…”

A multifunctional ratiometric electrochemical sensor for combined determination of indole-3-acetic acid and salicylic acid

“…The ratio of the two signals is used to replace the absolute value of signal of analyte as the output signal, thus the reproducibility and robustness of the sensor can be remarkably enhanced. Nowadays, ratiometric electrochemical sensors have been developed for a varieties of biomolecules including nucleic acid, 22,23 biological small molecule, 24,25 protein, 26,27 and metal ions, 28,29 etc. But most ratiometric electrochemical sensors only can detect one analyte.…”

Recent Advances in Protein Kinase Activity Analysis Based on Nanomaterials

“…AuNPs modified on the phosphorylated peptide through Au-SH bond acted as catalyst to accelerate the oxidation of luminol on the electrode surface. Moreover, the spectra overlap between the absorption band of AuNPs and the ECL emission band of GQDs facilitated the ECL resonance energy transfer between AuNPs and GODs [49]. Our group reported an ECL biosensor for PKA activity detection.…”