A dual-potential ratiometric electrochemiluminescence biosensor based on Au@CDs nanoflowers, Au@luminol nanoparticles and an enzyme-free DNA nanomachine for ultrasensitive p53 DNA detection

Wang, Li; Liu, Pengfei; Liu, Zhijun; Cao, Hai-Xia; Shuying, Ye; Zhao, Kai-Ren; Liang, Guoxi; Zhu, Jun‐Jie

doi:10.1016/j.snb.2020.128890

Cited by 35 publications

(14 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…59–61 Depending on the structure of the composites formed, there are mainly three forms of metal presented in the metal–CD composites, including metal ions/atoms, metal nanoparticles (MNPs) and metal oxides (MOs). 62–65…”

Section: Synthesis Of CD Compositesmentioning

confidence: 99%

“…For instance, in Yan and co-workers’ study, glyoxylic acid-modified CDs (GA-CDs) were used as a FRET ratiometric fluorescent probe for the detection of Cu 2+ in aqueous solution. 388 Similarly, Chen et al also applied cyclam-functionalized CDs as a FRET nanoprobe for the detection of Cu 2+ and S. 2–389 In addition to the above examples, the detection of other important metal ions such as Ag + , Cu 2+ , Cr 6+ , and Pb 2+ has also been successfully realized with CD composite-based sensing platforms. 390–401…”

Section: Composites For Biomedical Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Carbon dot composites for bioapplications: a review

Chen

Jia

et al. 2022

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

Section: Synthesis Of CD Compositesmentioning

confidence: 99%

Section: Composites For Biomedical Applicationsmentioning

confidence: 99%

Carbon dot composites for bioapplications: a review

Chen

Jia

et al. 2022

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…As they are a type of enzyme, polymerases can catalyze DNA and RNA synthesis. They can replicate DNA and form long, linear, tandem, or repetitive chains of DNA with the assistance of a polymerase enzyme from the DNA template, primers, and deoxy-ribonucleoside triphosphate (dNTP) [ 44 ]. Polymerase chain reaction (PCR) remains the traditional and the “gold standard” enzyme-assisted DNA amplification strategy in bioanalysis due to its high sensitivity and low cost [ 45 ].…”

Section: Dna-assisted Amplification Strategiesmentioning

confidence: 99%

“…Based on the intensity ratio of the two ECL peaks at different potentials, many ratiometric biosensors have been established for the detection of prostate-specific antigen (PSA), aflatoxin B1 (AFB1), dopamine, miRNA, metal cations, and cancer cells, which not only improve the sensitivity, but also avoid false-positive errors in complex matrices [ 24 , 82 ]. However, this kind of ratiometric biosensor also requires two coreactants in addition to additional luminophores [ 44 , 83 , 84 ], which increase the complexity of the whole experimental process. Some efforts have been made to develop single-luminophore-based ratiometric sensors [ 24 ].…”

Section: Ratiometric Strategiesmentioning

confidence: 99%

Strategies for Enhancing the Sensitivity of Electrochemiluminescence Biosensors

et al. 2022

View full text Add to dashboard Cite

Electrochemiluminescence (ECL) has received considerable attention as a powerful analytical technique for the sensitive and accurate detection of biological analytes owing to its high sensitivity and selectivity and wide dynamic range. To satisfy the growing demand for ultrasensitive analysis techniques with high efficiency and accuracy in complex real sample matrices, considerable efforts have been dedicated to developing ECL strategies to improve the sensitivity of bioanalysis. As one of the most effective approaches, diverse signal amplification strategies have been integrated with ECL biosensors to achieve desirable analytical performance. This review summarizes the recent advances in ECL biosensing based on various signal amplification strategies, including DNA-assisted amplification strategies, efficient ECL luminophores, surface-enhanced electrochemiluminescence, and ratiometric strategies. Sensitivity-enhancing strategies and bio-related applications are discussed in detail. Moreover, the future trends and challenges of ECL biosensors are discussed.

show abstract

“…Gold nanoparticles were shown to significantly increase the sensitivity of electrochemical biosensors because of the increased surface area for probe attachment and the conductivity of the electrode [30]. Our previous work has shown that electrodeposition of AuNPs as an underlying layer to chitosan-gold nano complex on interdigitated gold microelectrodes significantly increased the electrode's conductivity and therefore allowed for sensitive DNA detection [31].…”

Section: Introductionmentioning

confidence: 99%

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

O’Riordan

Wasiewska

Diaz

et al. 2022

Preprint

View full text Add to dashboard Cite

Shiga toxin-producing E. coli (STEC) is a food-borne pathogen of significant public health concern, due to the severity of the illness it can cause and a low infection dose. The key targets in molecular-based assays to detect STEC are stx1 or stx2 genes, coding for the ability to produce Shiga T Toxin 1 and 2. The most commonly used molecular detection techniques, such as PCR and real-time PCR, are considerably time-consuming and there is an urgent need for a more rapid screening assay which could be used in agri-food settings. In this work, an amplification-free multiplex electrochemical sensor for the simultaneous detection of stx1 and stx2 genes was developed using a silicon-based chip comprising of six interdigitated gold microelectrodes(IDE) sensors. Two probes complementary to stx1 and stx2 genes were immobilised on a single chip allowing for multiplex detection. The selectivity of the multiplex sensor was confirmed using fluorescence and electrochemistry. The developed assay conditions allowed for an improved limit of detection by three orders of magnitude compared to the previous reports achieving a amplification-free limit of detection of 100 zM (10-19 M) for both, stx1 and stx2 genes. The developed sensor was validated using chromosomal DNA extracted from a bacterial culture containing no virulence genes, stx1 gene only, and both stx1 and stx2. Such multiplex sensor, if combined with on-chip DNA extraction, could revolutionise the point-of-use detection of STEC as well as other pathogens for instance on-farm or in the food industry.

show abstract

A dual-potential ratiometric electrochemiluminescence biosensor based on Au@CDs nanoflowers, Au@luminol nanoparticles and an enzyme-free DNA nanomachine for ultrasensitive p53 DNA detection

Cited by 35 publications

References 47 publications

Carbon dot composites for bioapplications: a review

Carbon dot composites for bioapplications: a review

Strategies for Enhancing the Sensitivity of Electrochemiluminescence Biosensors

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC)

Contact Info

Product

Resources

About