A dual role of protein kinase C in insulin signal transduction via adenylyl cyclase signaling system in muscle tissues of vertebrates and invertebrates11Abbreviations: PKC, protein kinase C (α, λ, ε, ϕ, ζ, its isoforms); AC, adenylyl cyclase (EC 4.6.1.1); AC I, II, etc. (isoforms of AC); ACSM, adenylyl cyclase signaling mechanism; DAG, diacylglycerol; PE, phorbol esters; PMA, phorbol 12-myristate 13-acetate; PI3-K, phosphatidylinositol 3-kinase; Gs-protein, stimulatory GTP-binding protein; Gi-protein, inhi

Plesneva, S. A.; Ao, Shpakov; Kuznetsova, L. A.; Pertseva, M. N.

doi:10.1016/s0006-2952(01)00592-5

Cited by 38 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adenylyl cyclase (EC 4.6.1.1) activity was measured as described earlier [52]. The reaction mixture (final volume 50 μ L) contained 50 mM Tris-HCl (pH 7.4), 5 mM MgCl 2 , 1 mM ATP, 0.5–1 μ Ci [ α - 32 P]-ATP, 0.1 mM cAMP, 20 mM creatine phosphate, 0.2 mg/mL creatine phosphokinase, and 15–45 μ g of membrane protein.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

Derkach

Бондарева

Chistyakova

et al. 2015

International Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

In the last years the treatment of type 2 diabetes mellitus (DM2) was carried out using regulators of the brain signaling systems. In DM2 the level of the brain serotonin is reduced. So far, the effect of the increase of the brain serotonin level on DM2-induced metabolic and hormonal abnormalities has been studied scarcely. The present work was undertaken with the aim of filling this gap. DM2 was induced in male rats by 150-day high-fat diet and the treatment with low dose of streptozotocin (25 mg/kg) on the 70th day of experiment. From the 90th day, diabetic rats received for two months intranasal serotonin (IS) at a daily dose of 20 μg/rat. The IS treatment of diabetic rats decreased the body weight, and improved glucose tolerance, insulin-induced glucose utilization, and lipid metabolism. Besides, it restored hormonal regulation of adenylyl cyclase (AC) activity in the hypothalamus and normalized AC stimulation by β-adrenergic agonists in the myocardium. In nondiabetic rats the same treatment induced metabolic and hormonal alterations, some of which were similar to those in DM2 but expressed to a lesser extent. In conclusion, the elevation of the brain serotonin level may be regarded as an effective approach to treat DM2 and its complications.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

Derkach

Бондарева

Chistyakova

et al. 2015

International Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Peptide 1039–1061 induces a dose-dependent stimulation of GTP-binding and GTPase activities of G s proteins, while peptide 1325–1345 containing a site for tyrosine phosphorylation located in the C-terminal domain of IR activates the G i/o proteins [117], and peptide 1135–1156 containing tyrosine residues critical for IR tyrosine kinase activity stimulates GTP-binding of unusual 67 kDa G protein involved in the insulin signal transduction [118]. The G i -activating effect of C-terminal peptide 1325–1345 is supported by the data obtained later on direct interaction between insulin-activated IR with G i2 protein and the blocking of this interaction by PT [116, 119]. …”

Section: The Receptors With Tyrosine Kinase Activitymentioning

confidence: 99%

Signal Protein-Derived Peptides as Functional Probes and Regulators of Intracellular Signaling

Шпаков

2011

Journal of Amino Acids

Self Cite

View full text Add to dashboard Cite

The functionally important regions of signal proteins participating in their specific interaction and responsible for transduction of hormonal signal into cell are rather short in length, having, as a rule, 8 to 20 amino acid residues. Synthetic peptides corresponding to these regions are able to mimic the activated form of full-size signal protein and to trigger signaling cascades in the absence of hormonal stimulus. They modulate protein-protein interaction and influence the activity of signal proteins followed by changes in their regulatory and catalytic sites. The present review is devoted to the achievements and perspectives of the study of signal protein-derived peptides and to their application as selective and effective regulators of hormonal signaling systems in vitro and in vivo. Attention is focused on the structure, biological activity, and molecular mechanisms of action of peptides, derivatives of the receptors, G protein α subunits, and the enzymes generating second messengers.

show abstract

Cyclic AMP signaling in bivalve molluscs: an overview

Fabbri¹,

Capuzzo²

2010

J. Exp. Zool.

View full text Add to dashboard Cite

The cyclic AMP (cAMP)-dependent signaling accounts for the control of cellular cascades involved in many physiological functions, and a wealth of information is available on the cAMP system that operates in mammalian cells. Nevertheless, cAMP has a central role also in nonmammalian vertebrates and invertebrates. The present review aims at examining the information available on bivalve molluscs, from the first studies carried out in the early 1980s to the last progresses made in the present days. The major focus is on the structural and operational characteristics of the main actors of the signaling pathway, i.e., adenylyl cyclase, G proteins, and protein kinase A, and on the role played by the cyclic nucleotide on smooth muscle, heart, gills, gonads, and metabolism regulation. Moreover, recent evidence regarding the cAMP system as a target of environmental stress factors are discussed. It will become clear that cAMP does play a wide and important role in bivalve physiology. Several issues have been sufficiently clarified, although investigated only in a few model species. However, further fundamental aspects remain unknown, mainly regarding molecular features and interactions with other signaling pathways, thus requiring further elucidation. J. Exp. The discovery of cyclic AMP (cAMP) about 50 years ago was the crucial event leading to our current paradigm of second-messenger signaling, through which many hormones, neurotransmitters, odorants, and autocrine/paracrine regulators modulate physiological functions. Other second messengers were discovered from then on; however, cAMP has never left its center stage. Biological and genetic evidence point to roles for cAMP in a vast array of biological systems, including oogenesis and embryogenesis, development, hormone secretion, olfaction, cardiac contraction, smooth muscle relaxation, metabolism, etc. Therefore, understanding the cAMP-dependent pathway in all its parts became a scientific priority that prompted to a remarkable amount of studies and relevant achievements (Diel et al., 2008;Sadana and Dessauer, 2009). Admittedly, the pathway once considered simple and straightforward became very complex, thus requiring more and more sophisticated investigations mainly focused on mammalian cells. If this is easily understandable also for the implications of cAMP signaling in human health and possible therapeutic applications, the counterpart is a rather scarce knowledge of the system in nonmammalian vertebrates and invertebrates.The present review focuses on the cAMP-dependent pathway in bivalve molluscs, aimed at collecting and ordering the research outputs produced in this regard. Bivalve molluscs are the model animals currently studied in our laboratories, and we did experience such fragmented information. Moreover, growing interest is addressed to these animals as widely cultivated aquaculture species and sentinel organisms used for evaluating the environmental quality. Undoubtedly cAMP plays a role in these contexts and an organized set of information would be of be...

show abstract

Cited by 38 publications

References 61 publications

The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

The Effect of Long-Term Intranasal Serotonin Treatment on Metabolic Parameters and Hormonal Signaling in Rats with High-Fat Diet/Low-Dose Streptozotocin-Induced Type 2 Diabetes

Signal Protein-Derived Peptides as Functional Probes and Regulators of Intracellular Signaling

Cyclic AMP signaling in bivalve molluscs: an overview

Contact Info

Product

Resources

About