A dynamic matrix potentiates mesenchymal stromal cell paracrine function via an effective mechanical dose

Abstract

Paracrine function of mesenchymal stromal cells (MSCs) contributes a lot to tissue development, which is regulated by several physical factors. Moreover, extracellular matrix (ECM) of MSCs is dynamic and its...

Modulation of Inherent Niches in 3D Multicellular MSC Spheroids Reconfigures Metabolism and Enhances Therapeutic Potential

“…In addition to providing physical support for cell adhesion, these matrix proteins are known to regulate multiple cellular behaviors, including the migration, proliferation and differentiation of MSCs, via outside-in signaling [1,26,27]. Furthermore, a close correlation between the secretory activity of MSCs and their surrounding matrixes has been extensively reported in recent studies [28][29][30][31], suggesting that the niche established by the ECM can be critical for the therapeutic function of MSCs. Moreover, HSPGs are known to act as depots to entrap various soluble factors and to protect their bioactivity [32].…”

Design and aligner-assisted fast fabrication of a microfluidic platform for quasi-3D cell studies on an elastic polymer

“…Cells respond to various cues from the surrounding microenvironment, such as nanoscaled cues [ 51 , 52 ], surface topology [ 53 , 54 ], material scaffolding [ [55] , [56] , [57] ], biodegradation [ [58] , [59] , [60] ], and chemical cues [ 61 , 62 ]. And in many cases, mechanical cues should be taken into consideration [ [63] , [64] , [65] ]. It is challenging to reveal responses of individual cells to mechanical cues due to the lack of sufficient innovative material platforms to control mechanical stimulations in single-cell scales.…”

Recent advances in 3D hydrogel culture systems for mesenchymal stem cell-based therapy and cell behavior regulation

“…In both 2D and 3D dimensions, the nuclear localization of transcription coactivators YAP and TAZ and transcription factor protein RUNX2 are normal indicators for studying the effect of stiffness. 196,197 Hydrogels with a suitable stiffness not only maintain the self-renewal of MSCs, but also provide a microenvironment to guide MSCs to differentiate into a specific cell lineage. The increase of matrix stiffness would lead to increased cytoskeletal tension.…”

“…In 2020, it was reported that alginate hydrogel was employed to build a dynamic matrix exploring the effect of matrix stiffness on the paracrine of MSCs. 196 The dynamic matrix was created by treating hydrogels with calcium chloride solution at a specific time point during culture. The dynamic stiffness must be the future of designing relevant culture systems.…”