The parameters of reservoir impact all the sectors of oilfield developing after flood development, so methods of separate stratum injection allocation are researched for forecasting the petroleum reservoir performance accurately. The methods of separate stratum injection allocation are significative for remaining oil distribution, injection allocation of interval and the level of exploitation and administration in high water cut stage. First, we should derive injection-withdrawal ratio (IWR), gradient of pressure and water cut by material balance equation.The injection allocation of single well,injection wells and property of interval are determined by the research of split coefficient.We find the reservoir small error, lower water cut, high degree of reservoir recovery by anaysising the results of separate stratum injection allocation.The results show that separate stratum injection allocation is scientific and reasonable, simple and applied for the oil field in high water cut stage.