A facile and highly sensitive impedimetric DNA biosensor with ultralow background response based on in situ reduced graphene oxide

Wu, Anxin; Wang, Qingxiang; Zhu, Qionghua; Ni, Jiancong; Gao, Feng

doi:10.1039/c5ra16233e

Cited by 7 publications

(1 citation statement)

References 40 publications

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“…A calibration curve that quantitatively relates the change in charge-transfer resistance (ΔR ct ) in response to target DNA hybridization and its concentration was finally constructed. 11,18,39,49,50 Experimental Reagents and instrumentation.-Potassium hexacyanoferrate (III) (K 3 Fe(CN) 6 , >99.0%), potassium hexacyanoferrate(II) (K 2 Fe(CN) 6 , >98.5%), potassium chloride (>99.0%), 0.01 M phosphate-buffered saline (PBS; 0.138 M NaCl, 0.0027 M KCl, pH 7.4), tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl, >99.8%), anhydrous ethylenediaminetetraacetic acid (EDTA, >99.0%), and sodium chloride (NaCl, >99.5%) were purchased from Sigma-Aldrich and used as received. Ethanol (>99.9%), silver nitrate (AgNO 3 , >99.8%), and 50% glutaraldehyde were obtained from Daejung (Siheung, Korea).…”

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Tunable Electrochemical Grafting of Diazonium for Highly Sensitive Impedimetric DNA Sensor

Kang

Kim

Lee

et al. 2020

J. Electrochem. Soc.

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The development of a simple, rapid, and reliable method for detecting the virus at very low levels is therefore an important health objective. To that end, a gold surface was electrochemically modified by reducing 4-nitrobenzenediazonium tetrafluoroborate. The rate of radical formation and its polymerization were significantly affected by the reaction temperature, the concentration of the diazonium precursor solution, the number of cyclic voltammetry cycles, and the scan rate. The nitro functional group was subsequently electrochemically converted into the corresponding amine. In order to detect target DNA related to dengue virus, glutaraldehyde was used to link probe DNA to the amino functional group, after which the immobilized probe DNA was hybridized with various concentrations of target DNA. Ferri/ferrocyanide signals during each step in the overall transformation, including diazonium modification, reduction to the amine, probe DNA immobilization, and target DNA hybridization, were monitored by cyclic voltammetry and electrochemical impedance spectroscopy. We finally investigated the quantitative relationship between the change in charge-transfer resistance in response to target DNA hybridization and its concentration. This sensor responded linearly in the 100 pM to 1 nM concentration range, and its detection limit was determined to be 40.6 pM.

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confidence: 99%

Tunable Electrochemical Grafting of Diazonium for Highly Sensitive Impedimetric DNA Sensor

Kang

Kim

Lee

et al. 2020

J. Electrochem. Soc.

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Trends in the Development of Scanning Diagnostic Equipment for the Formation and the Development of Medical Visualization – Nucleic Acid Biosensors for Medical Diagnostics

Hasan

Gartia

2022

Encyclopedia of Materials: Plastics and Polymers

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Graphene oxide directed in-situ deposition of electroactive silver nanoparticles and its electrochemical sensing application for DNA analysis

Gao

et al. 2017

Analytica Chimica Acta

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A facile and highly sensitive impedimetric DNA biosensor with ultralow background response based on in situ reduced graphene oxide

Abstract: A facile and highly sensitive impedimetric DNA biosensor with ultralow background response based on in situ reduced graphene oxide.

Cited by 7 publications

References 40 publications

Tunable Electrochemical Grafting of Diazonium for Highly Sensitive Impedimetric DNA Sensor

Tunable Electrochemical Grafting of Diazonium for Highly Sensitive Impedimetric DNA Sensor

Trends in the Development of Scanning Diagnostic Equipment for the Formation and the Development of Medical Visualization – Nucleic Acid Biosensors for Medical Diagnostics

Graphene oxide directed in-situ deposition of electroactive silver nanoparticles and its electrochemical sensing application for DNA analysis

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