No abstract

DNA microarrays are used to examine changes in gene expression of a large number of genes simultaneously by fluorescent labeling of complementary DNAs (cDNAs).

Since 3D measurement technologies have been widely used in manufacturing industries edge detection in a depth image plays an important role in computer vision applications. In this paper, we have proposed an edge detection process in a depth image based on the image based smoothing and morphological operations. In this method we have used the principle of Median filtering, which has a renowned feature for edge preservation properties. The edge detection was done based on Canny Edge detection principle and was improvised with morphological operations, which are represented as combinations of erosion and dilation. Later, we compared our results with some existing methods and exhibited that this method produced better results. However, this method works in multiframe applications with effective framerates. Thus this technique will aid to detect edges robustly from depth images and contribute to promote applications in depth images such as object detection, object segmentation, etc. Highlights A method is proposed that can detect edges from depth images more profoundly. We modified the Canny edge detection method using morphological operations. The proposed method works in multi-frames.

Stem cell‐based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective treatment requires precise non‐destructive evaluation of the purity of stem cells with high sensitivity (<0.001% of the number of cells). Here, a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping‐based machine learning analysis is reported to distinguish differentiating human adipose‐derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enables identifying differentiated cells at single‐cell resolution. The label‐free HSI method is compared with the standard techniques such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. HSI is successfully used to assess the abundance of adipocytes derived from transplanted cells in a transgenic mice model. Further, Raman microscopy and multiphoton‐based metabolic imaging is performed to provide complementary information for the functional imaging of the hASCs. Finally, the HSI method is validated using matrix‐assisted laser desorption/ionization‐mass spectrometry imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label‐free identification of stem cell differentiation with high spatial and chemical resolution.