DNA microarray analysis using a smartphone to detect the BRCA-1 gene

Prasad, Alisha; Hasan, Syed Mohammad Abid; Grouchy, Steven; Gartia, Manas Ranjan

doi:10.1039/c8an01020j

Cited by 37 publications

(16 citation statements)

References 46 publications

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“…This reagent consists of an aromatic amine sulphanilamide which reacts with nitrite to form a diazonium salt, which then combines with N-(1-naphthyl)-ethylenediamine dihydrochloride (NEDD) to form a pink colored azo dye [27]. Citric acid assessment was based on acid-base titration [21], such that the reaction of citric acid with sodium hydroxide reaches an equivalence point, else it climbs above this point (Figure 3b). For ascorbic acid, the LOD was found to be 1.47 µM with a linear range from 1–20 µM (Figure 3a), for glucose LOD was 20 mM with a linear range from 10–40 mM (Figure 3d), and for nitrite LOD was 0.06 mM with a linear range from 0.30–1.40 mM (Figure 3c).…”

Section: Resultsmentioning

confidence: 99%

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Multiplexed Paper Microfluidics for Titration and Detection of Ingredients in Beverages

Prasad

Tran²,

Gartia

2019

Sensors

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Food safety and access to systematic approaches for ensuring detection of food hazards is an important issue in most developing countries. With the arrival of paper-based analytical devices (µPADs) as a promising, rapid, easy-to-use, and low-cost analytical tool, we demonstrated a simple microfluidic-based titration study for the analysis of packaged fruit juices. Similar, to the titration experiments using traditional glassware in chemistry laboratories, in this study the titration experiments were developed using paper microfluidics for the analysis of several analytes such as pH, vitamin C, sugars, and preservatives present in the packaged fruit juices. The allergen found commonly in dairy based mixtures and the non-pathogenic biochemical component responsible for food spoilage in cider based fruit juices were also determined. The results obtained using paper microfluidics were compared with those obtained using a conventional spectrophotometric technique. Finally, a paper microfluidics based multiplexed sensor was developed for the analysis of common nutritional ingredients, an allergen, and a non-pathogenic byproduct present in packaged fruit juices on a single platform. Overall, the results presented in this study reveal that the proposed paper microfluidic assisted colorimetric multiplexed sensor offers a quick and reliable tool for on-spot routine analysis for food safety applications.

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Section: Resultsmentioning

confidence: 99%

“…For demonstration of paper based titration study, several channels were designed using AutoCAD 2016, printed (Xerox ColorQube 8580/N Wax Printer, Xerox Corporation, Norwalk, CT, USA), and baked (for 2 min @ 120 °C) [21] (Quincy Lab 20GC Gravity Lab, Chicago, IL, USA) (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Multiplexed Paper Microfluidics for Titration and Detection of Ingredients in Beverages

Prasad

Tran²,

Gartia

2019

Sensors

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“…Summaries for the function of 4 significant genes. Biological information analysis has been widely applied to explore gene changes in the process of disease development, and this method may help scientists to identify new therapeutic targets [12][13][14] . A total of 371 DEGs, including 243 upregulated and 128 downregulated DEGs, were screened between NP cells cultured in hyperosmotic media and NP cells cultured in iso-osmotic media by analysing the biological data of the gene expression profile GSE1648.…”

Section: Sst Somatostatinmentioning

confidence: 99%

Identification of significant gene biomarkers of low back pain caused by changes in the osmotic pressure of nucleus pulposus cells

Zhao

Quan

et al. 2020

Sci Rep

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The incidence of intervertebral disc (IVD) degeneration disease, caused by changes in the osmotic pressure of nucleus pulposus (NP) cells, increases with age. In general, low back pain is associated with IVD degeneration. However, the mechanism and molecular target of low back pain have not been elucidated, and there are no data suggesting specific biomarkers of low back pain. Therefore, the research aims to identify and verify the significant gene biomarkers of low back pain. The differentially expressed genes (DEGs) were screened in the Gene Expression Omnibus (GEO) database, and the identification and analysis of significant gene biomarkers were also performed with various bioinformatics programs. A total of 120 patients with low back pain were recruited. Before surgery, the degree of pain was measured by the numeric rating scale (NRS), which enables comparison of the pain scores from individuals. After surgery, IVD tissues were obtained, and NP cells were isolated. The NP cells were cultured in two various osmotic media, including iso-osmotic media (293 mOsm/kg H 2 O) to account for the morbid environment of NP cells in IVD degeneration disease and hyper-osmotic media (450 mOsm/kg H 2 O) to account for the normal condition of NP cells in healthy individuals. The relative mRNA expression levels of CCL5, OPRL1, CXCL13, and SST were measured by quantitative real-time PCR in the in vitro analysis of the osmotic pressure experiments. Finally, correlation analysis and a neural network module were employed to explore the linkage between significant gene biomarkers and pain. A total of 371 DEGs were identified, including 128 downregulated genes and 243 upregulated genes. Furthermore, the four genes (CCL5, OPRL1, SST, and CXCL13) were identified as significant gene biomarkers of low back pain (P < 0.001) based on univariate linear regression, and CCL5 (odds ratio, 34.667; P = 0.003) and OPRL1 (odds ratio, 19.875; P < 0.001) were significantly related to low back pain through multivariate logistic regression. The expression of CCL5 and OPRL1 might be correlated with low back pain in patients with IVD degeneration disease caused by changes in the osmotic pressure of NP cells.IVD is fibro-cartilage tissue located between vertebrae and is composed of the nucleus pulposus, annulus fibrosus and endplate 1 . NP survives in a special ecological niche containing no distribution of blood vessels and nerves in which the osmotic pressure is significantly higher than that of plasma, enabling proteoglycans to be present in high concentrations 2,3 . Increasing with age, the osmotic pressure changes, and the interverbal disc degenerates 4,5 , which may be related to low back pain 6,7 . Previous studies have shown that the osmotic environment can influence the gene expression of NP cells 7 ; thus, we hypothesized that there are pain-related genes among these affected genes, which might provide new therapeutic strategies and drug targets for low back pain.In addition, the NRS is ubiquitous in the clinic as a means of evaluating low bac...

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“…Regarding the pathological diagnosis, a recent study showed that easy, fast, and high-quality image capturing and transfer are possible from cytology slides using smartphones, with high intraobserver Kappa agreement (84.3%) between the microscopic cytopathological diagnoses and remote smartphone image diagnoses [30]. Smartphone-based molecular analyses are also being developed [60,61,62].…”

Section: Potential Impacts Of E-health In Oncologymentioning

confidence: 99%

Outpatient Cancer Care Delivery in the Context of E-Oncology: A French Perspective on “Cancer outside the Hospital Walls”

Bertucci

Corroller

Monneur-Miramon

et al. 2019

Cancers

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In oncology, the treatment of patients outside of hospitals has become imperative due to an increasing number of patients who are older and live longer, along with issues such as medical desertification, oncologist hyperspecialization, and difficulties in financing mounting health expenditures. Treatments have become less “invasive”, with greater precision and efficiency. Patients can therefore receive most of their care outside of hospitals. The development of e-health can address these new imperatives. In this letter, we describe the different e-health tools and their potential clinical impacts in oncology, as already reported at every level of care, including education, prevention, diagnosis, treatment, and monitoring. A few randomized studies have yet demonstrated the clinical benefit. We also comment on issues and limits of “cancer outside the hospital walls” from the point of view of patients, health care professionals, health facilities, and public authorities. Care providers in hospitals and communities will have to adapt to these changes within well-coordinated networks in order to better meet patient expectations regarding increasing education and personalizing management. Ultimately, controlled studies should aim to definitively demonstrate areas of interest, benefits, and incentives, for not only patients, but also caregivers (formal and informal) and health care providers, health care facilities, and the nation.

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DNA microarray analysis using a smartphone to detect the BRCA-1 gene

Abstract: DNA microarrays are used to examine changes in gene expression of a large number of genes simultaneously by fluorescent labeling of complementary DNAs (cDNAs).

Cited by 37 publications

References 46 publications

Multiplexed Paper Microfluidics for Titration and Detection of Ingredients in Beverages

Multiplexed Paper Microfluidics for Titration and Detection of Ingredients in Beverages

Identification of significant gene biomarkers of low back pain caused by changes in the osmotic pressure of nucleus pulposus cells

Outpatient Cancer Care Delivery in the Context of E-Oncology: A French Perspective on “Cancer outside the Hospital Walls”

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