A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China

Abstract

A novel analytical protocol was developed for general quality screening of chicken meat based on IEF and protein extraction. To demonstrate the developed protocol, 24 chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water, and that in Group 3 were infected by new castle disease virus. Proteins were extracted from the meat samples by using pure water as an extractant, separated by IEF, verified by western blot, and quantified via imaging analysis. The relevant experiments demonstrated that two myoglobin (Mb) bands were detected at pI 6.8 and 7.04 for all samples of Groups 1, 2, and 3, but there were additional hemoglobin (Hb) bands at pI 7.09 and 7.13 (P < 0.05) for the samples of Groups 2 and 3. The results implied that Hb bands might be a potential biomarker for the screening of chicken meat quality. The RSD values of two Mb bands (pI 6.8 and 7.04) in Group 1 were respectively 4.08 and 3.63%, the ones of two Hb bands (pI 7.09 and 7.13) in Group 2 were 3.66 and 2.10%, and those in Group 3 were 2.17% and 2.77%, respectively. All the RSD values indicated high stability and reliability of the developed protocol. Additionally, the protocol had a direct readout of protein bands in IEF without staining. However, it was time‐consuming and had high cost. Even so, the relevant general method and finding have potential for screening of chicken meat quality.

Evaluation of Chinese Prickly Ash and Cinnamon to Mitigate Heterocyclic Aromatic Amines in Superheated Steam-Light Wave Roasted Lamb Meat Patties Using QuEChERS Method Coupled with UPLC-MS/MS

“…The samples were then analyzed using UPLC-MS/MS (Agilent model 1290). Mass spectrometric analyses were performed on an AB Sciex API 4000™ triple quadrupole mass spectrometer equipped with an electrospray ionization source to look for heterocyclic aromatic amines [15]. Separation was achieved on a Shim-pack GIST C18 (2.1 × 100 mm, 3 µm, 100 Å) at 37 • C. For further dilution, standard stock solutions containing 10 mg in 5 mL methanol were prepared.…”

Liquid-Phase Ion Trap for Ion Trapping, Transfer, and Sequential Ejection in Solutions

“…However, the ruling principles of ion motion in solutions are totally different from those in a vacuum environment. , Due to strong viscous damping effects in solutions, ions usually no longer possess characteristic motional frequencies within a rf quadrupole electric field. Instead, the electrophoretic force (or dielectrophoretic force for particles) would be dominant in solutions. , Isotachophoresis (ITP), − isoelectric focusing (IEF), − and electric field gradient focusing (EFGF) methods − have been developed and used primarily as separation techniques. In these methods, nonuniform solvents are required, so that either electric field gradients or electrolyte gradients could be generated inside liquid channels for ion separation.…”