A far‐red fluorescent contrast agent to image epidermal growth factor receptor expression

Hsu, Elizabeth R.; Anslyn, Eric V.; Dharmawardhane, Su; Alizadeh-Naderi, Reza; Aaron, Jesse; Sokolov, Konstantin V.; El‐Naggar, Adel K.; Gillenwater, Ann M.; Richards‐Kortum, Rebecca

doi:10.1111/j.1751-1097.2004.tb00395.x

Cited by 15 publications

(15 citation statements)

References 44 publications

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“…Considerations regarding the immunogenic effects of the antibody and in vivo use of the biotin-(strept)avidin system have previously been discussed in greater depth, 5 but will be briefly reviewed here. Immunogenic effects of the antibody in the contrast agent can be minimized through the use of low antibody concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…The contrast agent is described in detail in Hsu et al, 5 but is briefly reviewed here. The antibody used in the contrast agent was a biotinylated mouse monoclonal anti-human EGFR (clone 111.6, 200 lg/ml, LabVision NeoMarkers, Fremont, CA) and was used at a 1:10 dilution.…”

Section: Contrast Agentmentioning

confidence: 99%

“…Performance of contrast agent at physiological temperature MDA-MB-468 (an adenocarcinoma of the mammary gland known to overexpress EGFR at 1 3 10 6 receptors/cell, American Type Culture Collection, Manassas, VA) and SqCC/Y1 cells were grown in monolayer, as described in Hsu et al 5 Labeling was also performed as described in Hsu et al…”

Section: Recovery After Exposure To Alexa Flourmentioning

confidence: 99%

“…35 Previously, we demonstrated the efficacy of a far-red fluorescent contrast agent targeted against EGFR in squamous carcinoma cells of the buccal mucosa in monolayer culture and in 500-lm thick multilayer tissue constructs. 5 However, these efforts did not address the efficacy of this contrast agent in intact, living tissue from human patients. In our study, we evaluate the efficacy of this contrast agent for detection of early neoplasia using organ cultures of normal and neoplastic human oral tissue.…”

mentioning

confidence: 99%

“…We have developed a molecular-specific fluorescent contrast agent, consisting of a farred fluorescent dye coupled to a monoclonal antibody targeted against epidermal growth factor receptor (EGFR) to aid in noninvasive early detection of oral neoplasia in vivo. 5 EGFR is overexpressed in a high number of epithelial malignancies, including oral squamous carcinoma. The contrast agent is designed for topical application in vivo, followed by real time optical detection.…”

mentioning

confidence: 99%

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Real‐time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular‐specific contrast agent

Hsu

Gillenwater²,

Hasan

et al. 2006

Intl Journal of Cancer

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Early diagnosis of individuals with high risk of developing head and neck squamous carcinoma should lead to decreased morbidity and increased survival. To aid in noninvasive early detection of oral neoplasia in vivo, we have developed a molecular-specific fluorescent contrast agent, consisting of a far-red fluorescent dye coupled to a monoclonal antibody targeted against the epidermal growth factor receptor. In our study, we used organ cultures of normal and neoplastic human oral tissue to evaluate the capabilities of using this contrast agent to enhance clinical diagnosis. Fresh tissue sections were prepared from 34 biopsies of clinically normal and abnormal oral mucosa from 17 consenting patients. Samples were exposed to contrast agent, rinsed and the presence of bound agent was detected using fluorescence confocal microscopy. Simple assays to assess cytotoxicity of the dye used in the agent and to determine labeling efficacy at physiologic temperatures were also performed. Results indicate that the mean fluorescence intensity (MFI) of samples with dysplasia and cancer are higher than that of the normal sample from the same patient, and that this increase in fluorescence could potentially be used in the early detection and delineation of premalignant lesions. Normal tissue could be distinguished from cancer or moderate dysplasia, using either the ratio of the MFI of abnormal to normal tissue or the MFI obtained from the epithelial surface. No detrimental effects from the dye were observed over a 4-day period. These results indicate that the use of this optical contrast agent could yield important clinical advantages for noninvasive early detection and molecular characterization of oral mucosa. ' 2006 Wiley-Liss, Inc.Key words: fluorescent contrast agent; epidermal growth factor receptor; molecular-specific contrast agentThe 5-year survival rate for oral cancer has not improved significantly in the last 3 decades, despite numerous advances in treatment modalities.

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Section: Discussionmentioning

confidence: 99%

Section: Contrast Agentmentioning

confidence: 99%

Section: Recovery After Exposure To Alexa Flourmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Real‐time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular‐specific contrast agent

Hsu

Gillenwater²,

Hasan

et al. 2006

Intl Journal of Cancer

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Epithelial Pre‐cancers and Cancers, Optical Technologies for Detection and Diagnosis of

Carlson

Richards‐Kortum

2006

Wiley Encyclopedia of Biomedical Engineering

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In this article, the authors focus on optical technologies that have the potential to dramatically improve the detection and classification of epithelial precancers and cancers. Epithelial precancers are associated with a variety of morphologic and molecular changes, which currently can only be assessed through invasive, painful biopsy and subsequent histologic analysis. In addition to visual inspection and histology, a growing number of optical techniques are being applied to the detection of precancerous changes in vivo without the need for biopsy, and may serve as diagnostic tools in the future as diagnostic algorithms are further developed. Optical technologies are ideally suited to detect cancer‐related changes because they can noninvasively detect biochemical and morphologic changes with subcellular resolution throughout the entire epithelial thickness. With optical technologies, diagnosis can be achieved in real time using automated techniques. This article reviews recent developments in reflectance and fluorescence spectroscopy, reflectance and fluorescence confocal microscopy, optical coherence tomography, and optical coherence microscopy. For each emerging technology, the basic principles and the current and potential applications in early cancer detection and diagnosis are reviewed. The potential of optical molecular imaging using exogenous contrast agents to enhance molecular features within tissue that are important for precancer and cancer diagnosis are also reviewed. Finally, future improvements needed to advance optical technologies into widespread clinical use for precancer detection and diagnostic classification are outlined.

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Alexa Fluor 660 carboxylic acid succinimidyl ester

Sabnis¹

2015

Handbook of Fluorescent Dyes and Probes

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A far‐red fluorescent contrast agent to image epidermal growth factor receptor expression

Cited by 15 publications

References 44 publications

Real‐time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular‐specific contrast agent

Real‐time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular‐specific contrast agent

Epithelial Pre‐cancers and Cancers, Optical Technologies for Detection and Diagnosis of

Alexa Fluor 660 carboxylic acid succinimidyl ester

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