A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH) 2 D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS

Fabregat-Cabello, Neus; Farré-Segura, Jordi; Huyghebaert, Loreen; Peeters, Stéphanie; Goff, Caroline Le; Souberbielle, Jean‐Claude; Cavalier, Étienne

doi:10.1016/j.cca.2017.08.024

Cited by 40 publications

(14 citation statements)

References 24 publications

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“…Further, since conventional LC-MS analysis may not be able to distinguish molecules of the same mass as 25(OH)D 3 and may not be able to distinguish among molecules with similar fragmentation patterns, the concentration of 25(OH)D is often overestimated [41]. In contrast, radioimmunoassay, which is a better analytical method than LC-MS, exhibits good inter-rater reliability and can determine the concentrations of 25(OH)D 2 and 25(OH)D 3 simultaneously [42]. It appears to be the most economical technology for standardization in future research [43].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D deficiency as a risk factor for dementia and Alzheimer’s disease: an updated meta-analysis

et al. 2019

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Background: We aimed to comprehensively explore the associations between serum 25(OH)D deficiency and risk of dementia and Alzheimer's disease(AD). Methods: We systematically searched Pubmed, the Cochrane Library, Embase and the reference lists of pertinent review articles for relevant articles published from database inception up until January 2019. Pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated with random effects models using the Stata 12.0 statistical software package. Results: Twelve prospective cohort studies and four cross-sectional studies were included in this meta-analysis. The pooled HRs of dementia and AD, respectively, were 1.32 (95%CI: 1.16, 1.52) and 1.34 (95%CI: 1.13, 1.60) for vitamin D deficiency (< 20 ng/ml). In the subgroup analyses, the pooled HRs of dementia and AD, respectively, were 1.48 (95%CI: 1.19, 1.85) and 1.51 (95%CI: 1.04, 2.18) for moderate vitamin D deficiency (10-20 ng/ml) and 1.20 (95%CI: 0.99, 1.44) and 1.36 (95%CI: 1.01, 1.84) for severe vitamin D deficiency (< 10 ng/ml). Conclusion: There are significant associations between vitamin D deficiency and both dementia and AD. There are stronger associations between severe vitamin D deficiency (< 10 ng/ml) and both dementia and AD compared to moderate vitamin D deficiency (10-20 ng/ml).

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Section: Discussionmentioning

confidence: 99%

Vitamin D deficiency as a risk factor for dementia and Alzheimer’s disease: an updated meta-analysis

et al. 2019

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“…Many LC-MS/MS assays, on the other hand, experience difficulties distinguishing the two epimers due to their equal mass and their similar affinity for most LC columns, resulting in an overestimation, and potential misclassification, of total 25(OH)D [ 61 , 69 , 71 , 72 ]. Several LC-MS/MS assays now use non-C18 columns enabling them to chromatographically separate the epimer and measure it independently of 25(OH)D, despite the identical masses [ 45 , 68 , 73 , 74 , 75 , 76 ].…”

Section: How: Importance Of the Choice Of Methodsmentioning

confidence: 99%

“…This increases sensitivity considerably, yet unfortunately PTAD thwarts the separate quantification of epi-25(OH)D 3 . Without derivatisation, higher LOQs are reported, additional efforts in other areas have to be undertaken to acquire similar LOQs, or higher sample volumes are required [ 44 , 68 , 73 , 74 , 86 , 94 , 95 ].…”

Section: How: Importance Of the Choice Of Methodsmentioning

confidence: 99%

The When, What & How of Measuring Vitamin D Metabolism in Clinical Medicine

et al. 2018

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We now have the ability to measure a number of different vitamin D metabolites with very accurate methods. The most abundant vitamin D metabolite, 25-hydroxyvitamin D, is currently the best marker for overall vitamin D status and is therefore most commonly measured in clinical medicine. The added value of measuring metabolites beyond 25-hydroxyvitamin D, like 1,25-, and 24,25-dihydroxyvitamin D is not broadly appreciated. Yet, in some more complicated cases, these metabolites may provide just the information needed for a legitimate diagnosis. The problem at present, is knowing when to measure, what to measure and how to measure. For 25-hydroxyvitamin D, the most frequently used automated immunoassays do not meet the requirements of today’s standards for certain patient groups and liquid chromatography-tandem mass spectrometry is the desired method of choice in these individuals. The less frequently measured 1,25-dihydroxyvitamin D metabolite enables us to identify a number of conditions, including 1α-hydroxylase deficiency, hereditary vitamin D-resistant rickets and a number of granulomatous diseases or lymphoproliferative diseases accompanied by hypercalcaemia. Furthermore, it discriminates between the FGF23-mediated and non-FGF23-mediated hypophosphatemic syndromes. The 24,25-dihydroxyvitamin D metabolite has proven its value in the diagnosis of idiopathic infantile hypercalcaemia and has the potential of having value in identifying other diseases. For both metabolites, the understanding of the origin of differences between assays is limited and requires further attention. Nonetheless, in every way, appropriate measurement of vitamin D metabolism in the clinical laboratory hinges eminently on the comprehension of the value of the different metabolites, and the importance of the choice of method.

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“…There are several HPLC-MS/MS methods for analysis of vitamin D in human serum, plasma, dry blood spots and milk [16,19,[39][40][41][42][43][44][45][46][47][48][49][50][51][52]. Moreover, application of supercritical fluid chromatography (SFC) with MS/MS detection was reported [13,53].…”

Section: Hplc-ms/msmentioning

confidence: 99%

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

et al. 2020

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Vitamin D plays a role not only in the proper functioning of the skeletal system and the calcium-phosphate equilibrium, but also in the immune system, the cardiovascular system and the growth and division of cells. Although numerous studies have reported on the analysis of vitamin D status in various groups of patients, the clinical significance of measurements of vitamin D forms and metabolites remains ambiguous. This article reviews the reports analyzing the status of vitamin D in various chronic states. Particular attention is given to factors affecting measurement of vitamin D forms and metabolites. Relevant papers published during recent years were identified by an extensive PubMed search using appropriate keywords. Measurement of vitamin D status proved to be a useful tool in diagnosis and progression of metabolic syndrome, neurological disorders and cancer. High performance liquid chromatography coupled with tandem mass spectrometry has become the preferred method for analyzing the various forms and metabolites of vitamin D in biological fluids. Factors influencing vitamin D concentration, including socio-demographic and biochemical factors as well as the genetic polymorphism of the vitamin D receptor, along with vitamin D transporters and enzymes participating in vitamin D metabolism should be considered as potential confounders of the interpretation of plasma total 25(OH)D concentrations.

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A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH) 2 D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS

Cited by 40 publications

References 24 publications

Vitamin D deficiency as a risk factor for dementia and Alzheimer’s disease: an updated meta-analysis

Vitamin D deficiency as a risk factor for dementia and Alzheimer’s disease: an updated meta-analysis

The When, What & How of Measuring Vitamin D Metabolism in Clinical Medicine

Clinical Significance of Analysis of Vitamin D Status in Various Diseases

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