A fast, cost‐saving and sensitive method for determination of cefuroxime in plasma by HPLC with ultraviolet detection

Abstract

Cefuroxime (CFX) is a broad-spectrum second-generation cephalosporin and one of the best choices for antibiotic prophylaxis. However, when used in critically ill patients, it may present changes in its pharmacokinetic properties. Therefore, therapeutic drug monitoring of CFX is necessary for effective dosing strategies. A

Quantification and Determination of Stability of Tylvalosin in Pig Plasma by Ultra-High Liquid Chromatography with Ultraviolet Detection

“…To evaluate the linearity, calibration curves were established following a previously described method [ 23 ]. The linearity ranged from 0.1 µg/mL to 5 µg/mL and was described by the equation y = 0.9993x + 0.0021 (r 2 ≥ 0.9998).…”

“…Tylvalosin was analyzed according to the FDA recovery test [ 24 ]. A brief explanation can be found in [ 23 ]. The values ranged from 89.66 to 96.92% with a variability (CV) of <7.75%.…”

“…Several C18 columns available in the laboratory were also checked in [ 23 ]. The Brisa LC2 C18 column 250 mm × 4.6 mm i.d, 5 µm, was chosen as it possessed the best symmetrical peaks.…”

“…The liquid chromatography (LC) system, column and guard column were described in a previous paper [ 23 ].…”