2020
DOI: 10.1016/j.aquaculture.2019.734784
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A fast sex detection method for the Whiteleg shrimp Litopenaeus vannamei by post-PCR high resolution melting (HRM)

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Cited by 9 publications
(5 citation statements)
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“…At least three important genes were described in the process of sexual determination in L. vannamei: sexlethal (Sxl) [46], Pvfem-1 [47] and the 25749_180 loci [21]. The Sxl gene was reported during embryogenesis and gametogenesis and some isoforms were more abundant expressed in male adult testis (Sxl-5 and Sxl-6) while others in the female oocytes (Sxl-2 and Sxl-4).…”
Section: Discussionmentioning
confidence: 99%
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“…At least three important genes were described in the process of sexual determination in L. vannamei: sexlethal (Sxl) [46], Pvfem-1 [47] and the 25749_180 loci [21]. The Sxl gene was reported during embryogenesis and gametogenesis and some isoforms were more abundant expressed in male adult testis (Sxl-5 and Sxl-6) while others in the female oocytes (Sxl-2 and Sxl-4).…”
Section: Discussionmentioning
confidence: 99%
“…As in other aquaculture species, the Paci c white shrimp presents sexual dimorphism in which females may have higher growth rate in comparison to males [20]. The genetic mechanism of sex determination in L. vannamei and other penaeids was already studied and seems to present a ZZ/ZW model [19,21]. This model implies that males are homogametic (ZZ) and females heterogametic (ZW).…”
Section: Introductionmentioning
confidence: 99%
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“…The HRM assays detect PCR amplicon quantity differences resulting from one sex containing one copy of the target sequence template (heterozygous) and the other sex containing two copies of the target sequence template (homozygous). This technique can be used to detect sex‐linked sequences that are homozygous in one sex and heterozygous in the other, as well as SNPs associated with sex (England et al ., 2021; Matsunaga et al ., 2014; Perez‐Enriquez et al ., 2020). In addition, since HRM assays are useful in the detection of amplicon sequence differences, they can detect primer sequence homology and PCR fidelity (Aralar et al ., 2020).…”
Section: Methodsmentioning
confidence: 99%
“…Along with rapid advances of molecular genetics and genomic sequencing, numerous sex-specific or sex chromosome-linked DNA markers have been successfully screened and identified from a wide range of more than 100 aquatic species including fish, testudines, amphibia, echinodermata, decapod, and shellfish (Table 1) by different genetic and genomic methods, such as restriction fragment length polymorphism (RFLP) (Devlin et al, 1991), random amplified polymorphic DNA (RAPD) (Chen et al, 2009), suppression subtractive hybridization (SSH) (Chen et al, 2010), microsatellite or simple sequence repeats (SSR) (Sakamoto et al, 2000), amplified fragment length polymorphism (AFLP) (Dan et al, 2013;Ezaz et al, 2004;Griffiths et al, 2000;Ma et al, 2010;Olmstead et al, 2011;Pan et al, 2015;Wang et al, 2009a;Wang et al, 2009b), fine and linkage mapping of sex quantitative trait locus (QTL) or sex determination (SD) locus (Kamiya et al, 2012;Liu et al, 2020;Sun et al, 2014;Viñas et al, 2012;Yu et al, 2017;Zhang et al, 2019b), putative sex-linked and sexrelated gene sequence comparison or next-generation sequencing (NGS)-based and high-resolution melting (HRM) typing system (Gao et al, 2020;Ou et al, 2017;Perez-Enriquez et al, 2020;Yang et al, 2020), genome sequencing and re-sequencing (Han et al, 2020;Lin et al, 2017a;Liu et al, 2018;Xiao et al, 2020;Zhang et al, 2017a), comparative transcriptome and RNA sequencing (RNA-seq) (Lamatsch et al, 2015;Sun et al, 2018), restriction-site associated DNA sequencing (RAD-seq) (Fang et al, 2020;Lange et al, 2020;…”
Section: Identification and Application Of Sex-specific Or Sex Chromo...mentioning
confidence: 99%