A faster Rubisco with potential to increase photosynthesis in crops

Lin, Myat T.; Occhialini, Alessandro; Andralojc, P. J.; Parry, M. A. J.; Hanson, Maureen R.

doi:10.1038/nature13776

Cited by 416 publications

(332 citation statements)

References 30 publications

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“…At the same time, efforts to engineer photosynthesis, in particular the rate-limiting CO 2 -fixing reactions, are gaining momentum (47). To effectively and efficiently harness the potential of synthetic biology, it is essential to carefully characterize the diversity of CO 2 -fixation modules that are found in the vast treasure troves of metagenomic studies (48).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the heterooligomeric red-type rubisco activase from red algae

Loganathan

Tsai

Mueller‐Cajar

2016

Proc. Natl. Acad. Sci. U.S.A.

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The photosynthetic CO 2 -fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco) is inhibited by nonproductive binding of its substrate ribulose-1,5-bisphosphate (RuBP) and other sugar phosphates. Reactivation requires ATP-hydrolysis-powered remodeling of the inhibited complexes by diverse molecular chaperones known as rubisco activases (Rcas). Eukaryotic phytoplankton of the red plastid lineage contain so-called red-type rubiscos, some of which have been shown to possess superior kinetic properties to green-type rubiscos found in higher plants. These organisms are known to encode multiple homologs of CbbX, the α-proteobacterial red-type activase. Here we show that the gene products of two cbbX genes encoded by the nuclear and plastid genomes of the red algae Cyanidioschyzon merolae are nonfunctional in isolation, but together form a thermostable heterooligomeric Rca that can use both α-proteobacterial and red algal-inhibited rubisco complexes as a substrate. The mechanism of rubisco activation appears conserved between the bacterial and the algal systems and involves threading of the rubisco large subunit C terminus. Whereas binding of the allosteric regulator RuBP induces oligomeric transitions to the bacterial activase, it merely enhances the kinetics of ATP hydrolysis in the algal enzyme. Mutational analysis of nuclear and plastid isoforms demonstrates strong coordination between the subunits and implicates the nuclearencoded subunit as being functionally dominant. The plastid-encoded subunit may be catalytically inert. Efforts to enhance crop photosynthesis by transplanting red algal rubiscos with enhanced kinetics will need to take into account the requirement for a compatible Rca.rubisco | activase | photosynthesis | AAA + proteins | red algae

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Section: Discussionmentioning

confidence: 99%

Characterization of the heterooligomeric red-type rubisco activase from red algae

Loganathan

Tsai

Mueller‐Cajar

2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Although plant chloroplasts clearly originated from a cyanobacterial progenitor through endosymbiosis, none contain these devices, making their introduction an attractive target for improving plant photosynthetic efficiency. An important advance toward this goal was the introduction of a carboxysome protein and functional cyanobacterial Rubisco into a land plant that led to the aggregation of Rubisco within chloroplasts as occurs during carboxysome biogenesis (31).…”

Section: Targets Of Opportunitymentioning

confidence: 99%

Redesigning photosynthesis to sustainably meet global food and bioenergy demand

Ort

Merchant

Alric

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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812

628

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The world's crop productivity is stagnating whereas population growth, rising affluence, and mandates for biofuels put increasing demands on agriculture. Meanwhile, demand for increasing cropland competes with equally crucial global sustainability and environmental protection needs. Addressing this looming agricultural crisis will be one of our greatest scientific challenges in the coming decades, and success will require substantial improvements at many levels. We assert that increasing the efficiency and productivity of photosynthesis in crop plants will be essential if this grand challenge is to be met. Here, we explore an array of prospective redesigns of plant systems at various scales, all aimed at increasing crop yields through improved photosynthetic efficiency and performance. Prospects range from straightforward alterations, already supported by preliminary evidence of feasibility, to substantial redesigns that are currently only conceptual, but that may be enabled by new developments in synthetic biology. Although some proposed redesigns are certain to face obstacles that will require alternate routes, the efforts should lead to new discoveries and technical advances with important impacts on the global problem of crop productivity and bioenergy production.light capture/conversion | carbon capture/conversion | smart canopy | enabling plant biotechnology tools | sustainable crop production Increasing demands for global food production over the next several decades portend a huge burden on the world's shrinking farmlands. Increasing global affluence, population growth, and demands for a bioeconomy (including livestock feed, bioenergy, chemical feedstocks, and biopharmaceuticals) will all require increased agricultural productivity, perhaps by as much as 60-120% over 2005 levels (e.g., refs. 1 and 2), putting increased productivity on a collision course with environmental and sustainability goals (3). The 45 y from 1960 to 2005 saw global food production grow ∼160%, mostly (135%) by improved production on

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“…The transgenic tobacco was able to grow at high CO2 concentrations. This work was the first step to implement the carbon concentrationg mechanisms from cyanobacterial to tobacco, with the potential of increasing its photosynthetic efficiency (Lin et al, 2014). Genkov et al replaced the small subunit of Chlamydomonas RuBisCO with that of plants (e.g., spinach, Arabidopsis, sunflower).…”

Section: Engineering the Co2-fixation Pathway By Enhancing Co2-fixingmentioning

confidence: 99%

“…However, construction of such a cycle was still restricted by uncertainties in the expression, activity, stability, and regulation of all enzymes in this pathway. Recently, relocation of natural CO2-fixation pathways has (Kumar et al, 2009) Replaced the tobacco RuBisCO with cyanobacteria RuBisCO and observed significantly increased growth rate of tobacco under high concentration of CO2 2014 (Lin et al, 2014) Constructed a hybrid RuBisCO from different RuBisCO large and small subunits and studied its enzymatic properties - (Genkov et al, 2010;Ishikawa et al, 2011) Reported that over-expressing the sedoheptulose-1-7 bisphosphatase improves photosynthetic carbon gain and yield 2011 (Rosenthal et al, 2011) received much attention, as engineering natural CO2-fixing autotrophic microbes is usually difficult. In 2013, Mattozzi et al divided the 16 steps of the 3-hydroxypropionate cycle from Chloroflexus aurantiacus into four sub-pathways and expressed each sub-pathway in Escherichia coli (Mattozzi et al, 2013).…”

Section: Design and Relocation Of Co2-fixation Pathwaymentioning

confidence: 99%

Synthetic biology for CO2 fixation

Gong

Zhang

2016

Sci. China Life Sci.

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Recycling of carbon dioxide (CO2) into fuels and chemicals is a potential approach to reduce CO2 emission and fossil-fuel consumption. Autotrophic microbes can utilize energy from light, hydrogen, or sulfur to assimilate atmospheric CO2 into organic compounds at ambient temperature and pressure. This provides a feasible way for biological production of fuels and chemicals from CO2 under normal conditions. Recently great progress has been made in this research area, and dozens of CO2-derived fuels and chemicals have been reported to be synthesized by autotrophic microbes. This is accompanied by investigations into natural CO2-fixation pathways and the rapid development of new technologies in synthetic biology. This review first summarizes the six natural CO2-fixation pathways reported to date, followed by an overview of recent progress in the design and engineering of CO2-fixation pathways as well as energy supply patterns using the concept and tools of synthetic biology. Finally, we will discuss future prospects in biological fixation of CO2.carbon dioxide fixation, synthetic biology, CO2-fixation pathway, energy supply Citation:

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A faster Rubisco with potential to increase photosynthesis in crops

Cited by 416 publications

References 30 publications

Characterization of the heterooligomeric red-type rubisco activase from red algae

Characterization of the heterooligomeric red-type rubisco activase from red algae

Redesigning photosynthesis to sustainably meet global food and bioenergy demand

Synthetic biology for CO2 fixation

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