A feature point identification method for positron emission particle tracking with multiple tracers

Wiggins, Cody; Santos, Roque; Ruggles, Arthur E.

doi:10.1016/j.nima.2016.10.057

Cited by 31 publications

(21 citation statements)

References 26 publications

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“…If the nearby cells are of very similar activity, it is possible for the cells to be confused and switched during the tracking process. A brief discussion of this effect is found in [ 11 ]. If a sample cannot be prepared with well-separated cells, an application must be chosen such that occasional confusion of tracked cells will not detrimentally affect results.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, the observation of trajectories stretching across many frames indicates that measured trajectories are not likely due to noise. Past work has shown that no false positive trajectories were observed when accepted trajectories were limited to those at least 20 time steps in length [ 11 ]. In this work, the shortest observed trajectory is 27 frames in length.…”

Section: Discussionmentioning

confidence: 99%

“…Another adaptation of the line density method was developed by Wiggins et al . [ 11 ] and is used herein. A brief description follows.…”

Section: Methodsmentioning

confidence: 99%

“…Measurement biases are currently being explored as part of a separate work. Further discussion of this method, including a comparison of the FPI and G-means methods for PEPT, is found in [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…Another adaptation of the method of Bickell et al . is introduced in [ 11 ] and is described and used herein.…”

Section: Introductionmentioning

confidence: 99%

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Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Langford¹,

Wiggins²,

Santos³

et al. 2017

PLoS ONE

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A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18 F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Another adaptation of the line density method was developed by Wiggins et al . [ 11 ] and is used herein. A brief description follows.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Another adaptation of the method of Bickell et al . is introduced in [ 11 ] and is described and used herein.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Langford¹,

Wiggins²,

Santos³

et al. 2017

PLoS ONE

Self Cite

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show abstract

Positron emission particle tracking in pulsatile flow

2017

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Integrated positron emission particle tracking (PEPT) and X-ray computed tomography (CT) imaging of flow phenomena in twisted tape swirl flow

Wiggins,

Cabral,

Mafi

et al. 2024

Exp Fluids

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A combined positron emission particle tracking (PEPT) and X-ray computed tomography (CT) technique is presented, and its utility is demonstrated through investigation of flow in a pipe with twisted tape swirl insert with varying flow conditions (diameter-based Reynolds numbers 16,300–63,300). A description of this technique is given, as well as data handling practices used to relate geometric information captured by CT to fluid flow data gathered via PEPT. It is found that the CT component is readily capable of capturing the stainless steel insert geometry in this present system, but the use of combined plastic and metal materials leads to artifacts in imaging of the plastic surface. Nonetheless, CT data are related to PEPT flow measurements, and average velocity fields are calculated via a pseudo-framing and interpolation scheme and used to visualize and interrogate key flow phenomena within the system. Radial velocity profiles of the mean flow characteristics are seen to collapse to a nearly common form across all flow conditions considered. Helical vortices are seen propagating through the flow field, generated by bypass flow around the gap between the insert and pipe wall, with additional coherent secondary flow structures seen in the higher Reynolds number cases. These findings enhance the understanding of the mixing mechanisms in these swirl flows and encourage the continued development of PEPT-CT methodologies for 3D flow measurements in optically inaccessible systems.

show abstract

A feature point identification method for positron emission particle tracking with multiple tracers

Cited by 31 publications

References 26 publications

Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Positron emission particle tracking in pulsatile flow

Integrated positron emission particle tracking (PEPT) and X-ray computed tomography (CT) imaging of flow phenomena in twisted tape swirl flow

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