A Ferrocene‐Tagged Amyloid‐β Fragment for Rapid Screening of Aggregation Inhibitors from Natural Compounds by HPLC‐Electrochemical Detection

Zhou, Lin; Kai, Tianhan; Sun, Zhifang; Hao, Yuanqiang; Tu, Qiuyun; Zhou, Feimeng

doi:10.1002/elan.201300025

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…The findings were validated using fluorescence imaging using ThT dye as an imaging agent for β-A peptides (Veloso and Kerman 2012). Electrochemical detection of β-A in combination with HPLC was explored by Zhang et al via monitoring oxidation of Fc, attached to N-terminus to KLVFFAE, inhibiting the aggregation of β-A 1–42 (Zhang et al 2013a). In this work, authors used curcumin as an electroactive component to inhibit β-A aggregation.…”

Section: Advancements Of Electrochemical β-A Bio-sensingmentioning

confidence: 99%

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Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Kaushik

Jayant

Tiwari

et al. 2016

Biosensors and Bioelectronics

154

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Beta-amyloid (β-A) peptides are potential biomarkers to monitor Alzheimer’s diseases (AD) for diagnostic purposes. Increased β-A level is neurotoxic and induces oxidative stress in brain resulting in neurodegeneration and causes dementia. As of now, no sensitive and inexpensive method is available for β-A detection under physiological and pathological conditions. Although, available methods such as neuroimaging, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) detect β-A, but they are not yet extended at point-of-care (POC) due to sophisticated equipments, need of high expertise, complicated operations, and challenge of low detection limit. Recently, β-A antibody based electrochemical immuno-sensing approach has been explored to detect β-A at pM levels within 30–40 minutes compared to 6–8 hours of ELISA test. The introduction of nano-enabling electrochemical sensing technology could enable rapid detection of β-A at POC and may facilitate fast personalized health care delivery. This review explores recent advancements in nano-enabling electrochemical β-A sensing technologies towards POC application to AD management. These analytical tools can serve as an analytical tool for AD management program to obtain bio-informatics needed to optimize therapeutics for neurodegenerative diseases diagnosis management

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Section: Advancements Of Electrochemical β-A Bio-sensingmentioning

confidence: 99%

“…In this work, authors used curcumin as an electroactive component to inhibit β-A aggregation. HPLC-electrochemical enabled separation of soluble Fc-KLVFFAE molecules from curcumin to control aggregation in real time manner (Zhang et al 2013a). …”

Section: Advancements Of Electrochemical β-A Bio-sensingmentioning

confidence: 99%

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Kaushik

Jayant

Tiwari

et al. 2016

Biosensors and Bioelectronics

154

View full text Add to dashboard Cite

show abstract

“…Amyloidosis is caused by certain proteins creating insoluble fibrous aggregates referred to as amyloid fibrils. Amyloidosis includes more than 20 kinds of diseases such as Alzheimer's disease and prion diseases, and there are many studies on amyloid fibrils using both experiments (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and simulations (for reviews, see, e.g., (50,51)).…”

Section: Introductionmentioning

confidence: 99%

Structural Analysis of a Trimer of β2-Microgloblin Fragment by Molecular Dynamics Simulations

Nishikawa

Sakae

Gouda

et al. 2019

Biophysical Journal

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A peptide b 2 -m 21À31 , which is a fragment from residue 21 to residue 31 of b 2 -microgloblin, is experimentally known to self-assemble and form amyloid fibrils. In order to understand the mechanism of amyloid fibril formations, we applied the replica-exchange molecular dynamics method to the system consisting of three fragments of b 2 -m 21À31 . From the analyses on the temperature dependence, we found that there is a clear phase transition temperature in which the peptides aggregate with each other. Moreover, we found by the free energy analyses that there are two major stable states: One of them is like amyloid fibrils and the other is amorphous aggregates.

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Electroanalytical Sensors and Methods for Assays and Studies of Neurological Biomarkers

et al. 2014

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Diverse methods have been developed to reveal biochemical changes in neurological disorders and to detect biomarkers present in clinical samples. Electrochemical biosensors are attractive because of their low costs, high sensitivity, and easiness for miniaturization. They have the potential for both point‐of‐care and in vivo monitoring. With clever surface modification and effective immobilization of capture ligands, selective detection of neurological biomarkers can be achieved. This review covers select applications of electroanalytical methods/sensors for proteins, peptides, small molecules, metal ions, and neurotransmitters. Designs of various sensors and devices are reviewed and issues to be addressed for clinically viable sensors are discussed.

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A Ferrocene‐Tagged Amyloid‐β Fragment for Rapid Screening of Aggregation Inhibitors from Natural Compounds by HPLC‐Electrochemical Detection

Cited by 5 publications

References 52 publications

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Nano-biosensors to detect beta-amyloid for Alzheimer's disease management

Structural Analysis of a Trimer of β2-Microgloblin Fragment by Molecular Dynamics Simulations

Electroanalytical Sensors and Methods for Assays and Studies of Neurological Biomarkers

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