A fibronectin mimetic motif improves integrin mediated cell biding to recombinant spider silk matrices

Widhe, Mona; Shalaly, Nancy Dekki; Hedhammar, My

doi:10.1016/j.biomaterials.2015.10.013

Cited by 77 publications

(130 citation statements)

References 67 publications

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“…Interestingly, Widhe et al reported that changing the widely used cell attachment RGD ( i.e. , arginine-glycine-asparagine) motive using genetic engineering to a constellation more similar to that of fibronectin improves attachment, spreading, stress fiber formation and focal adhesions [29], which are crucial factors for cell migration. Scott et al demonstrated that inhibition of fibronectin assembly strongly reduces the contractile force generation of human embryonic fibroblasts [30].…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Coating Materials on Viability and Migration of Schwann Cells

Klein

Prantl

Vykoukal³

et al. 2016

Materials

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Synthetic nerve conduits have emerged as an alternative to guide axonal regeneration in peripheral nerve gap injuries. Migration of Schwann cells (SC) from nerve stumps has been demonstrated as one essential factor for nerve regeneration in nerve defects. In this experiment, SC viability and migration were investigated for various materials to determine the optimal conditions for nerve regeneration. Cell viability and SC migration assays were conducted for collagen I, laminin, fibronectin, lysine and ornithine. The highest values for cell viability were detected for collagen I, whereas fibronectin was most stimulatory for SC migration. At this time, clinically approved conduits are based on single-material structures. In contrast, the results of this experiment suggest that material compounds such as collagen I in conjunction with fibronectin should be considered for optimal nerve healing.

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Section: Discussionmentioning

confidence: 99%

Differential Effects of Coating Materials on Viability and Migration of Schwann Cells

Klein

Prantl

Vykoukal³

et al. 2016

Materials

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“…Its material properties have likewise been manipulated through additions such as carbon nanotubes for hardening20 and poly(lactic-co-glycolic acid) to modify biological degradability21. Efforts have also been made to create silk-based chimeric proteins with sequences sourced from elastin22, collagen232425, and Fn, particularly the RGD (Arg-Gly-Asp) cell binding domain262728, all with the intent to improve native silk bioactivity for biological applications. However, recombinant protein production comes with a substantial methodological barrier while only recapitulating a small degree of function from their parent proteins, such as the adhesive and viability differences of the RGD peptide versus full-length Fn2930.…”

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confidence: 99%

Silk-fibronectin protein alloy fibres support cell adhesion and viability as a high strength, matrix fibre analogue

Jacobsen

Rim

et al. 2017

Sci Rep

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Silk is a natural polymer with broad utility in biomedical applications because it exhibits general biocompatibility and high tensile material properties. While mechanical integrity is important for most biomaterial applications, proper function and integration also requires biomaterial incorporation into complex surrounding tissues for many physiologically relevant processes such as wound healing. In this study, we spin silk fibroin into a protein alloy fibre with whole fibronectin using wet spinning approaches in order to synergize their respective strength and cell interaction capabilities. Results demonstrate that silk fibroin alone is a poor adhesive surface for fibroblasts, endothelial cells, and vascular smooth muscle cells in the absence of serum. However, significantly improved cell attachment is observed to silk-fibronectin alloy fibres without serum present while not compromising the fibres’ mechanical integrity. Additionally, cell viability is improved up to six fold on alloy fibres when serum is present while migration and spreading generally increase as well. These findings demonstrate the utility of composite protein alloys as inexpensive and effective means to create durable, biologically active biomaterials.

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“…Silk tube self-assembly Silk encapsulation of the scaffold was achieved using recombinantly produced miniaturized spider silk fusion proteins containing either a domain capable of binding IgG (Z) [8] or an integrin-binding motif from fibronectin (FN) [9] (Z-4RepCT and FN-4RepCT, Spiber Technologies, Sweden). The encapsulation process is shown in detail in the "Silk self-assembly" part of Figure 1.…”

Section: Figure 2: A) Photograph Of the Micromachined Scaffold; B) Brmentioning

confidence: 99%

Formation of a thin-walled spider silk tube on a micromachined scaffold

Guo

Gustafsson

Jansson

et al. 2018

2018 IEEE Micro Electro Mechanical Systems (MEMS)

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A fibronectin mimetic motif improves integrin mediated cell biding to recombinant spider silk matrices

Cited by 77 publications

References 67 publications

Differential Effects of Coating Materials on Viability and Migration of Schwann Cells

Differential Effects of Coating Materials on Viability and Migration of Schwann Cells

Silk-fibronectin protein alloy fibres support cell adhesion and viability as a high strength, matrix fibre analogue

Formation of a thin-walled spider silk tube on a micromachined scaffold

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