A field evaluation of serological and cellular diagnostic tests for bovine tuberculosis

Wood, P.R.; Corner, L. A.; Rothel, J.S.; Ripper, Jan; Fifis, Theodora; McCormick, B. S.; Francis, B.R.; Melville, L.; Small, Katherine; Witte, Kai-Erik; Tolson, Jonathan; Ryan, T.J.; Lisle, Geoffrey W. de; Cox, John C.; Jones, Stephen L.

doi:10.1016/0378-1135(92)90142-g

Cited by 138 publications

(84 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this case, this common population was also small (40.9%). Therefore, and in agreement with other authors [13,28,32,39,45], the detection of the maximum number of infected animals is achieved by the combination of both tests. Both tests were complementary also in the way that the IDTB test performed better during the first part of the trial, while the IFN-γ test was the only method that detected the infected animals at the second part of the study (it detected the M. bovis culture positive cattle that failed to react to the IDTB test).…”

Section: Discussionsupporting

confidence: 91%

“…Although it is difficult to compare the results obtained in the published field trials because of the use of different methodology (sampling time, different PPD and ELISA kit), source of cattle, and criteria of interpretation, the sensitivity values obtained in this study for both the IDTB and the IFN-γ tests have been only moderate, and in general terms, they were lower than those found in other reports [13,18,28,31,32,45]. This low performance is more obvious regarding the IFN-γ assay since it is generally accepted to have a higher sensitivity.…”

Section: Discussioncontrasting

confidence: 82%

“…An animal was considered positive for bovine tuberculosis when the mean OD of the sample stimulated with bovine PPD minus the mean OD of nil antigen was greater that 0.050 [44,45]. Other criteria previously used by other authors have been applied only to allow the comparison of the results obtained in this field trial with other scientific publications.…”

Section: Ifn-γ Testmentioning

confidence: 99%

“…In most of them, the sensitivity of the IFN-γ test was higher compared to the tuberculin tests [13,18,24,28,32,44,45]. The specificity of the IFN-γ assay was 90.6 to 98.6% [3,20,44].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

et al. 2006

View full text Add to dashboard Cite

-The intradermal tuberculin (IDTB) test and the interferon-gamma (IFN-γ) assay are used worldwide for detection of bovine tuberculosis in cattle, but little is known about the effect of co-infecting agents on the performance of these diagnostic tests. This report describes a field trial conducted in a cattle herd with dual infection (bovine tuberculosis and paratuberculosis) during 3.5 years. It has been based on a strategic approach encompassing serial parallel testing (comparative IDTB test, the IFN-γ assay and serology of paratuberculosis) that was repeated 8 times over the period, and segregation of animals into two herds. The IDTB test detected 65.2% and the IFN-γ test detected 69.6% of the Mycobacterium bovis culture-positive cattle. However, the IDTB test performed better during the first part of the trial, while the IFN-γ test was the only method that detected infected animals during the following three samplings. The number of false positive reactors with the IDTB and/or the IFN-γ tests was remarkably high compared to other reports, and could be caused by cross-reactivity with M. avium subsp. paratuberculosis. Also, the M. bovis isolates from cattle and wildlife from the same property were characterised using molecular techniques to disclose an epidemiological link. The IDTB test may not be appropriate to eradicate bovine tuberculosis in herds with dual mycobacterial infections. This report highlights the need to use several diagnostic techniques for the accurate detection of M. bovis infected animals in these herds. tuberculosis / paratuberculosis / IDTB / IFN-γ / eradication

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 82%

Section: Ifn-γ Testmentioning

confidence: 99%

“…In most of them, the sensitivity of the IFN-γ test was higher compared to the tuberculin tests [13,18,24,28,32,44,45]. The specificity of the IFN-γ assay was 90.6 to 98.6% [3,20,44].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Two M. bovis antigens, MPB70 and MPB64, have previously been identified as both T-and B-cell targets in M. bovis-infected cattle [19][20][21]. The limited species distribution and the broad recognition of these antigens by tuberculous cattle suggests that they have potential for inclusion in an improved diagnostic test.…”

Section: Introductionmentioning

confidence: 99%

T‐Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis

Mackie

et al. 1998

Scand J Immunol

View full text Add to dashboard Cite

Lightbody KA, Girvin RM, Mackie DP, Neill SD, Pollock JM. T-Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis. Scand J Immunol 1998;48:44-51 Defined antigenic reagents and knowledge of T-cell responses are required for the design of improved diagnostic tests for bovine tuberculosis. The limited species distribution of Mycobacterium bovis antigens MPB70 and MPB64 has indicated their potential for inclusion in future tests. The strategy adopted in this study was to define bovine T-cell responses to these antigens at the epitope level, using cattle immunized with recombinant forms of the antigens, and to compare these responses with cattle which had been experimentally infected with M. bovis. Panels of synthetic peptides (20-mers with 10-residue overlaps) were used and five epitopes were identified and found to be powerful stimulators of T-cell responses in both types of animal (residues 81-100 and 174-190 for MPB70; and residues 1-20, 41-60 and 181-200 for MPB64). Further investigation in larger numbers of cattle (n ¼ 14) of mixed breeds from tuberculosisinfected herds confirmed that each peptide produced response in several of the cattle, but no single peptide was recognized by all animals. However, the limited numbers of animals in this study suggest that peptide reagents may identify as many positive animals as the intact antigenic protein and could form components of a future diagnostic test. The use of cattle immunized with the proteins of interest has proved to be an interesting model for studying the nature of bovine T-cell responses to defined mycobacterial proteins.

show abstract

Comparison of a Whole-Blood Interferon γ Assay With Tuberculin Skin Testing for Detecting Latent <EMPH TYPE="ITAL">Mycobacterium tuberculosis</EMPH> Infection

Mazurek

LoBue

Daley

et al. 2001

JAMA

306

190

View full text Add to dashboard Cite

show abstract

A field evaluation of serological and cellular diagnostic tests for bovine tuberculosis

Cited by 138 publications

References 13 publications

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected withMycobacterium bovisandM. aviumsubsp.paratuberculosis

T‐Cell Recognition of Mycobacterial Proteins MPB70 and MPB64 in Cattle Immunized with Antigen and Infected with Mycobacterium bovis

Comparison of a Whole-Blood Interferon γ Assay With Tuberculin Skin Testing for Detecting Latent <EMPH TYPE="ITAL">Mycobacterium tuberculosis</EMPH> Infection

Contact Info

Product

Resources

About