A Film Tension Theory of Phagocytosis

Chen, Hongming; Langer, Róbert; Edwards, David A.

doi:10.1006/jcis.1997.4865

Cited by 55 publications

(25 citation statements)

References 7 publications

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“…Moreover, the interfacial free energy was noted to be positively related to the surface potential, underlining the increase of phagocytosis with the increase of negative zeta potential. On the other hand, the phagocytosis was facilitated when nanoparticles were more hydrophobic than the cell [33].…”

Section: The Hsa Nonspecific Binding Of Msns and Pegylated Msns By Pementioning

confidence: 92%

The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

et al. 2010

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Section: The Hsa Nonspecific Binding Of Msns and Pegylated Msns By Pementioning

confidence: 92%

The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

et al. 2010

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“…Several studies have been conducted to define the role of size in phagocytosis (22)(23)(24)(25)(26)(27). Tabata and Ikada studied phagocytosis of polystyrene microspheres (0.5-4.6 μm) by mouse peritoneal macrophages and reported that maximal phagocytosis was observed for an intermediate particle size (1.7 μm) (23).…”

Section: Nih Public Accessmentioning

confidence: 99%

“…Simon studied the uptake of 0.5-8 μm polystyrene microspheres by human blood neutrophils and leukocytes and reported that phagocytosis decreased with increasing particle size (24). Theoretical work has also been performed to understand the dependence of phagocytosis on particle size (27). These studies predicted that phagocytosis should increase with particle size for hydrophobic particles and decrease for hydrophilic particles.…”

Section: Nih Public Accessmentioning

confidence: 99%

Role of Particle Size in Phagocytosis of Polymeric Microspheres

2008

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Purpose-Polymeric microspheres are extensively researched for applications in drug and vaccine delivery. However, upon administration into the body, microspheres are primarily cleared via phagocytosis by macrophages. Although numerous studies have reported on the biochemical pathways of phagocytosis, relatively little is known about the dependence of phagocytosis on particle size. Here, we investigate the previously unexplained dependence of phagocytosis on particle size.Methods-Rat alveolar macrophages and IgG-opsonized and non-opsonized polystyrene microspheres were used as model macrophages and drug delivery particles. Phagocytosis, attachment and internalization were measured by flow cytometry and time-lapse video microscopy.Results-Particles possessing diameters of 2-3 μm exhibited maximal phagocytosis and attachment. Rate of internalization, however, was not affected significantly by particle size. Maximal attachment of 2-3 μm microspheres is hypothesized to originate from the characteristic features of membrane ruffles in macrophages. Elimination of ruffles via osmotic swelling nearly eliminated the peculiar size-dependence of phagocytosis. A simple mathematical model is presented to describe the dependence of phagocytosis on particle size.Conclusions-The dependence of phagocytosis on particle size originated primarily from the attachment step. These results reveal the importance of controlling drug delivery particle size distribution and selecting the size appropriate for avoiding or encouraging phagocytosis.

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“…The phagocytotic capacity (% volume of cell occupied by particles) will depend on the various size-shape descriptors as mentioned before like ECD, AR and R 12,23,24 . Previous investigators have studied the role of size in phagocytosis, both biological and theoretical 25 . Simon et al, studied the phagocytosis of polystyrene microspheres (0.5-8.0 µm) by human blood neutrophils and found that phagocytosis decreased with increasing particle size 20 .…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis of Simulated Nano-wear Debris by Osteoblasts

Musib¹

2012

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In this study I have used well characterized simulated wear-nano-particles of ultra-high molecular weight polyethylene (UHMWPE) of known size and shape to study particulate phagocytosis by MG63 osteoblast-like cells. The particles were treated to decrease their propensity to form aggregates in aqueous suspension and scanning electron microscopy (SEM) was performed to image them as individual particles on 0.01 µm pore size polycarbonate filter membranes. These images were further subjected to morphometric analysis of the particles using ASTM F1877 descriptors [equivalent circle diameter (ECD in μm), aspect ratio (AR), elongation (E), roundness (R), and formfactor (FF)]. The mean (±SD) ECD of the particles were 0.056±0.03 µm. They were subsequently introduced to confluent MG63 cells at particle:cell ratio of 100:1 and incubated for 24 h. Transmission electron micrography (TEM) showed the nanoparticles inside the cytosol. No time dependent response was observed beyond 24 h. The nanoparticles seemed to re-agglomerate once inside the cell. The damage to the cells was evident from the compromised cell membrane. This study will help further our knowledge of the wear-mediated osteolytic process.

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A Film Tension Theory of Phagocytosis

Cited by 55 publications

References 7 publications

The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

The effect of PEGylation of mesoporous silica nanoparticles on nonspecific binding of serum proteins and cellular responses

Role of Particle Size in Phagocytosis of Polymeric Microspheres

Phagocytosis of Simulated Nano-wear Debris by Osteoblasts

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