A Fine Structure Study of Lipid in Mouse Liver Regenerating After Partial Hepatectomy

Abstract

The fine structure of liver 3½ to 72 hours after partial hepatectomy has been compared with that of liver from sham-operated animals; all animals were 60- to 90-day old male mice of the C3H strain. Numerous small bodies with diameters ranging from 300 to 1,000 A have been observed distributed randomly throughout the cytoplasm of the hepatic parenchymal cells at early intervals after partial hepatectomy. In material fixed in osmium tetroxide and embedded in methacrylate, they appear as uniformly electron-opaque bodies, but in permanganate-fixed liver, they display only a peripheral rim of electron-opaque material surrounding a clear core. Each of these cytoplasmic bodies appears to be located within a vesicle. A few of the opaque bodies are also present in sinusoids and in the spaces of Disse; these bodies are not located within vesicular structures. Fat droplets of various sizes are easily distinguished in regenerating liver; with the increase in number of these fat droplets at later postoperative intervals, there occurs a concomitant decrease in the number of cytoplasmic bodies. It is suggested that the cytoplasmic bodies contain some lipid component. Possible explanations of the origin, nature, and fate of the cytoplasmic bodies are discussed.

Association of SND1 protein to low density lipid droplets in liver steatosis

“…During this process, the liver suffers a transient accumulation of large amounts of fat. Such fat is stored in cytosolic LD, which proliferate and grow after the partial hepatectomy [34].…”

To divide or not to divide: revisiting liver regeneration

“…It is well known that hepatocytes accumulate massive amounts of lipids and glycogen immediately after 70% PHx [49-51]. Therefore, the rapid increase in hepatocyte size is at least partly due to lipid and glycogen accumulation.…”

Some Observations on the Fine Structure of the Human Liver in Congenital Biliary Atresia

“…Even if it is postulated that the majority of the liver cells in biliary atresia would arise by regeneration, none of the studies of the ultrastructure of regenerating cells after partial hepatectomy, or of hepatoma has referred to the anomalous microvillous borders (ATERMAN, 1961;FISHER and FISHER, 1963;TROTTER, 1964). In the biliary atresia hepatocyte, frequent displacement of nucleus to the lateral cell surface and the structure which resembles the intracellular bile canaliculi are noticed, both of which have not been described previously in any type of hepatic lesions.…”