A flexible culture process for production of the green microalga Scenedesmus dimorphus rich in protein, carbohydrate or lipid

Wang, Liang; Li, Yunguang; Sommerfeld, Milton R.; Hu, Qiang

doi:10.1016/j.biortech.2012.10.062

Cited by 86 publications

(38 citation statements)

References 22 publications

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“…However, lipid production with dual N sources (BG11+SABCr) is similar to photoautotrophic conditions (BG11), suggesting that mixotrophic resources do not appreciably enhance lipid production through a ''heterotrophic boost'' in this alga when nitrate is available, which contrasts to other proposed algal systems (Park et al, 2012;Zhao et al, 2012). It is also possible that cells might be delayed in lipid production when excessive nitrogen inputs (BG11+SABCr) are provided, as lipid biosynthesis is dependent on the cellular growth phase (Wang et al, 2013).…”

Section: Effects Of Fermentation Residues On Cellular Compositionmentioning

confidence: 96%

“…Cells were grown in parallel in 40 ml of media in 125 ml Erlenmeyer flasks using four different nitrogen regimes (nitrate, nitrate depleted, nitrate + yeast extracts, and yeast extracts without nitrate) for 14 days, and their final dry weights determined at the end of this growth period (Table 1). It should be noted that although cells grown in nitrate depleted media were used as a negative control, noticeable biomass increases were still observed among these cultures, indicating that these cells likely have intracellular nitrogen reserves to support biomass accumulation and limited cell divisions after resuspension in nitrogen depleted media (Wang et al, 2013).…”

Section: Preliminary Strain Selection With Complex N Sourcesmentioning

confidence: 99%

“…These efforts were designed to mimic a real-world growth and extraction regime to test whether nitrogen in the extracted residues could support the growth of a new inoculum. S. acutus cells were first grown under high carbohydrate accumulating conditions and harvested for processing (Laurens et al, 2014;Wang et al, 2013). Mild acid hydrolysis (2% H 2 SO 4 in water; 150°C) was used to lyse cells and hydrolyze biomass.…”

Section: Algal Fermentation Residuesmentioning

confidence: 99%

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Nitrogen recycling from fuel-extracted algal biomass: Residuals as the sole nitrogen source for culturing Scenedesmus acutus

Nagle

Pienkos

et al. 2015

Bioresource Technology

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In this study, the reuse of nitrogen from fuel-extracted algal residues was investigated. The alga Scenedesmus acutus was found to be able to assimilate nitrogen contained in amino acids, yeast extracts, and proteinaceous alga residuals. Moreover, these alternative nitrogen resources could replace nitrate in culturing media. The ability of S. acutus to utilize the nitrogen remaining in processed algal biomass was unique among the promising biofuel strains tested. This alga was leveraged in a recycling approach where nitrogen is recovered from algal biomass residuals that remain after lipids are extracted and carbohydrates are fermented to ethanol. The protein-rich residuals not only provided an effective nitrogen resource, but also contributed to a carbon "heterotrophic boost" in subsequent culturing, improving overall biomass and lipid yields relative to the control medium with only nitrate. Prior treatment of the algal residues with Diaion HP20 resin was required to remove compounds inhibitory to algal growth.

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Section: Effects Of Fermentation Residues On Cellular Compositionmentioning

confidence: 96%

Section: Preliminary Strain Selection With Complex N Sourcesmentioning

confidence: 99%

Section: Algal Fermentation Residuesmentioning

confidence: 99%

See 1 more Smart Citation

Nitrogen recycling from fuel-extracted algal biomass: Residuals as the sole nitrogen source for culturing Scenedesmus acutus

Nagle

Pienkos

et al. 2015

Bioresource Technology

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“…For instance, Baky et al (2012) found a dried weight of 1.651 g L -1 for S. obliquus cultivated at 25 °C in N-9 culture medium at 200 µE m -2 s -1 and 9% CO 2 in the gas line. Wang et al (2013) …”

Section: Resultsmentioning

confidence: 99%

“…Several works relying on its cultivation have investigated a variety of aspects, including types of culture medium and substrate, which have been tested in bench or continuous systems, also employing different intensities of light. However, studies on the efficiency of biomass recycling coupled to the photobioreactor either through simulation or experimental data are still required (Vigeolas et al, 2012;Yin-Hu et al, 2012;Baky et al, 2013;Wang et al, 2013;Wu et al, 2013;Lee et al, 2013 - θ is an operating limit, below which the biomass cannot be maintained in the system because the wash-out rate is higher than the growth rate. From the fact that 1 …”

Section: Introductionmentioning

confidence: 99%

Simulation of Microalgal Growth in a Continuous Photobioreactor With Sedimentation and Partial Biomass Recycling

Silva

Gris

Bertucco

2016

Braz. J. Chem. Eng.

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-Microalgae are considered as promising feedstocks for the third generation of biofuels. They are autotrophic organisms with high growth rate and can stock an enormous quantity of lipids (about 20 -40% of their dried cellular weight). This work was aimed at studying the cultivation of Scenedesmus obliquus in a two-stage system composed of a photobioreactor and a settler to concentrate and partially recycle the biomass as a way to enhance the microalgae cellular productivity. It was attempted to specify by simulation and experimental data a relationship between the recycling rate, kinetic parameters of microalgal growth and photobioreactor operating conditions. Scenedesmus obliquus cells were cultivated in a lab-scale flat-plate reactor, homogenized by aeration, and running in continuous flow with a residence time of 1.66 day. Experimental data for the microalgal growth were used in a semi-empirical simulation model. The best results were obtained for 0.2, when R = 1 and k d = 0 and 0.05 day -1 , with the biomass production in the reactor varying between 8 g L -1 and 14 g L -1 , respectively. The mathematical model fitted to the microalgal growth experimental data was appropriate for predicting the efficiency of the reactor in producing Scenedesmus obliquus cells, establishing a relation between cellular productivity and the minimum recycling rate that must be used in the system.

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Biofuels from Microalgae: Bioethanol

Bastos

2018

Green Energy and Technology

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A flexible culture process for production of the green microalga Scenedesmus dimorphus rich in protein, carbohydrate or lipid

Cited by 86 publications

References 22 publications

Nitrogen recycling from fuel-extracted algal biomass: Residuals as the sole nitrogen source for culturing Scenedesmus acutus

Nitrogen recycling from fuel-extracted algal biomass: Residuals as the sole nitrogen source for culturing Scenedesmus acutus

Simulation of Microalgal Growth in a Continuous Photobioreactor With Sedimentation and Partial Biomass Recycling

Biofuels from Microalgae: Bioethanol

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