A Flow-Injection Biosensor System for the Amperometric Determination of Creatinine: Simultaneous Compensation of Endogenous Interferents

Rui, Chang Sheng; Sonomoto, Kenji; Ogawa, Hiroaki; Kato, Yasuhiko

doi:10.1006/abio.1993.1168

Cited by 17 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A few papers have reported matrixes such as soup or dehydrated broths and a few by using flow injection methodology (Gutie ´rrez et al 1989;Del Campo et al 1998;Rui et al 1993;Campins-Falco et al 2001). In these papers the sample was treated by the official method.…”

Section: Introductionmentioning

confidence: 99%

Automated Method for the Total Creatinine Determination in Dehydrated Broths

et al. 2006

View full text Add to dashboard Cite

In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe ´reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7 h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h 21 , and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.

show abstract

Section: Introductionmentioning

confidence: 99%

Automated Method for the Total Creatinine Determination in Dehydrated Broths

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Substantial improvements in creatinine biosensor sensitivity and fabrication have been accomplished by employing ammonium ion-selective membrane electrodes 10 or amperometric ammonia sensors as signal-transducing elements. Nevertheless, the use of such a detection scheme still has inherent problems in accuracy, reliability, and construction due to the interfering effect of endogenous ammonia present in natural specimens. 10a,11b, Particular consideration also has been given to the enzyme catalytic sequence proposed by Tsuchida and Yoda, which is based on the three-enzyme system consisting of creatinine amidohydrolase (creatininase, CA), creatine amidinohydrolase (creatinase, CI), and sarcosine oxidase (SO): Most creatinine biosensors incorporating such biocatalytic reactions have been coupled with the detection of the hydrogen peroxide ultimately generated. − However, the assay of creatinine still remains one of the most challenging analytical problems, owing to the possible interferences and the presence of creatine in serum at nearly the same concentrations as those of creatinine.…”

mentioning

confidence: 99%

A Planar Amperometric Creatinine Biosensor Employing an Insoluble Oxidizing Agent for Removing Redox-Active Interferences

et al. 2001

View full text Add to dashboard Cite

A planar microchip-based creatinine biosensor employing an oxidizing layer (e.g., a PbO2 film), where interfering redox-active substances are broken (i.e., oxidized) to redox-inactive products, was developed to facilitate the microfabrication of the sensor and to provide improved, reliable determination of creatinine in physiological samples. The feasibility of using hydrophilic polyurethanes in permselective barrier membranes for creatinine biosensors and the effect of adding a silanizing agent (adhesion promoter) on the sensor performance (e.g., sensitivity, stability, and lifetime) are described. The proposed creatinine microsensor with a three-layer configuration, i.e., enzyme, protecting, and oxidizing layers, exhibits good electrochemical performance in terms of response time (t95% = 98 s at 100-->200 microM creatinine change), linearity (1-1000 microM, r = 0.9997), detection limit (0.8 microM), and lifetime (approximately 35 days). The creatinine biosensor devised in a differential sensing arrangement that compensates the erroneous results from creatine is considered to be suitable for assay of serum specimens.

show abstract