A fluorescence-based biosensor for the detection of organophosphate pesticides and chemical warfare agents

Viveros, Leamon; Paliwal, Sheetal; McCrae, David A.; Wild, James R.; Simonian, Aleksandr

doi:10.1016/j.snb.2005.08.032

Cited by 121 publications

(62 citation statements)

References 29 publications

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“…The recognition element (OPH) and reporter (CNF) molecules were designed to entertain OP samples with concentrations of neurotoxin as low as 0.05 μM. Quantitative detection could be determined from 1 to 800 μM for paraoxon and from 2 to 400 μM for DFP (Viveros et al 2006). Gold nanoparticle based surface enhanced fluorescence (NSEF) spectroscopy for rapid and sensitive screening of organophosphorus agents (OPA) was reported.…”

Section: Fluorescence Based Detection Of Pesticides and Organo-phosphmentioning

confidence: 99%

Organophosphorous Compounds-Toxicity and Detection Approach

Upadhyay¹,

Sharma²,

Rao³

et al. 2011

Pesticides - Strategies for Pesticides Analysis

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Section: Fluorescence Based Detection Of Pesticides and Organo-phosphmentioning

confidence: 99%

Organophosphorous Compounds-Toxicity and Detection Approach

Upadhyay¹,

Sharma²,

Rao³

et al. 2011

Pesticides - Strategies for Pesticides Analysis

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“…The OPH/SNAFL/PEG sensors investigated allowed the detection of paraoxon in aqueous solution, in concentrations as low as 10 À7 m, with an estimated detection limit (defined as a signal to noise ratio of three) of 10 À8 m. Sensory systems that rely on OPH/fluorescent dye systems have the potential to be used in portable electronic devices. Viveros et al [19] reported an OPH biosensor in which the fluorescent dye carboxynaphthofluorescein (CNF, Scheme 2c) was covalently bound to the enzyme. The complex was reported to operate well in combination with a fiber-optic biosensor assay.…”

Section: Enzyme-based Sensorsmentioning

confidence: 99%

Fluorescent Sensors for the Detection of Chemical Warfare Agents

Burnworth

Rowan

Weder

2007

Chemistry A European J

254

130

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Along with biological and nuclear threats, chemical warfare agents are some of the most feared weapons of mass destruction. Compared to nuclear weapons they are relatively easy to access and deploy, which makes them in some aspects a greater threat to national and global security. A particularly hazardous class of chemical warfare agents are the nerve agents. Their rapid and severe effects on human health originate in their ability to block the function of acetylcholinesterase, an enzyme that is vital to the central nervous system. This article outlines recent activities regarding the development of molecular sensors that can visualize the presence of nerve agents (and related pesticides) through changes of their fluorescence properties. Three different sensing principles are discussed: enzyme-based sensors, chemically reactive sensors, and supramolecular sensors. Typical examples are presented for each class and different fluorescent sensors for the detection of chemical warfare agents are summarized and compared.

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“…5 A number of methods have been developed in the last few years for the detection of organophosphorus pesticides. The most widely used methods are gas chromatography (GC), [6][7][8] high-performance liquid chromatography (HPLC), 9,10 gas chromatography-mass spectrometry (GC-MS), 11 immune assay and fluorescence, 12,13 chemiluminescence (CL). [14][15][16] Different methods like HPLC, GC and GC-MS have been reported but despite the precision and accuracy of these methods, analyses are restricted to laboratory facilities, are time-consuming and expensive due to its analytical cost, limiting the operation of these instruments to highly qualified laboratory persons.…”

Section: Introductionmentioning

confidence: 99%

A catalytic kinetic spectrophotometric determination of organophosphorus pesticides in vegetable samples

Tiwari¹,

Asthana²

2012

J. Braz. Chem. Soc.

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Um método cinético catalítico espectrofotométrico, simples, seletivo e sensível para a determinação de quantidade traço de pesticidas organofosforados foi proposto. O método é baseado no efeito catalítico de pesticidas organofosforados (malationa, dimetoato e forato) na oxidação de LCV (leuco cristal violeta) por iodato de potássio em meio de ácido clorídrico, produzindo um corante violeta. O corante apresenta absorção máxima em 592 nm. O método de tempo fixo foi usado por 15 min. O sistema obedece a lei de Beer no intervalo de 0,02-0,2, 0,032-0,32 e 0,03-0,3 µg mL -1 , para malationa, dimetoato e forato, respectivamente. Parâmetros analíticos importantes como tempo, temperatura, concentração do reagente, acidez, etc, foram otimizados para reação completa. A sensibilidade de Sandell e absortividade molar para o sistema foram 0,0002, 0,0004, 0,0004 µg cm -2 e 1,2 × 10 6 , 5,21 × 10 5 , 6,3 × 10 5 L mol -1 cm -1 , respectivamente. O método proposto foi aplicado satisfatoriamente à determinação de pesticidas organofosforados, em micro concentrações, em amostras vegetais.A simple selective and sensitive catalytic kinetic spectrophotometric method for the determination of trace amount of organophosphorus pesticides has been proposed. The method is based on the catalytic effect of organophosphorus pesticides (malathion, dimethoate and phorate) on the oxidation of LCV (leuco crystal violet) by potassium iodate in hydrochloric acid medium to give a violet colored dye. The dye shows maximum absorption at 592 nm. The fixed-time method was used for 15 min. The system obeys Beer's law in the range of 0.02-0.2, 0.032-0.32 and 0.03-0.3 µg mL -1 , for malathion, dimethoate, and phorate, respectively. Important analytical parameters such as time, temperature, reagent concentration, acidity etc, have been optimized for complete color reaction. Sandell's sensitivity and molar absorptivity for the system were found to be 0.0002, 0.0004, 0.0004 µg cm -2 and 1.2 × 10 6 , 5.21 × 10 5 , 6.3 × 10 5 L mol -1 cm -1 , respectively. The proposed method was satisfactorily applied to micro-level determination of organophosphorus pesticides in vegetable samples.

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A fluorescence-based biosensor for the detection of organophosphate pesticides and chemical warfare agents

Cited by 121 publications

References 29 publications

Organophosphorous Compounds-Toxicity and Detection Approach

Organophosphorous Compounds-Toxicity and Detection Approach

Fluorescent Sensors for the Detection of Chemical Warfare Agents

A catalytic kinetic spectrophotometric determination of organophosphorus pesticides in vegetable samples

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