A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)

Radotić, Ksenija; Melø, Thor Bernt; Lindgren, Mikael

doi:10.1016/j.saa.2023.123269

Cited by 2 publications

(4 citation statements)

References 23 publications

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“…For instance, Tradescantia spathacea and Tradescantia pallida display significant differences in pigment concentration, spanning chlorophylls, carotenoids, anthocyanins, and flavonoids (Figures 1-12). Although pigment concentrations disparities between these species are not as pronounced as those in other genera, such as Euphorbia, Croton, Phyllanthus, Acalypha, where pigment concentrations in green or purple leaves differ twentyfold, our results still confirm considerable variation [28,87]. Both species manifested similar scattering traits in the mid-infrared region but exhibited greater variations in the SWIR domain, concordant with other plants where leaf structural features influence light scattering, notwithstanding structural and ultrastructural alterations in plant cells.…”

Section: Adaptations To Cell and Tissue Structures From Spectral Datasupporting

confidence: 78%

“…The spectral signatures principally mirror pigment composition (VIS-NIR) and structural adjustments at both the cellular and ultrastructural levels. Chlorophyll fluorescence primarily indicates the photosynthetic efficiency of leaf surface cells, thereby emphasising the functional implications of morphological variation [ 2 , 28 , 30 , 66 , 67 ]. By measuring the fluorescence emitted by a flash or detection between two sensors that penetrated the mesophyll tissue profile, we ascertained that the internal leaf signals diminished upon the sensor return [ 1 , 11 , 14 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the integration of correlation by hyperspectral, chlorophyll a fluorescence and microscopic techniques demonstrated that pigment distribution and leaf architecture synergistically affected the light management strategies of Tradescantia species [ 8 , 10 , 11 ]. By examining spectral indices and cellular details, different mechanisms by which structural modifications at the cellular level enhance light-energy management in plants can be identified, for example, deconvolution or hyperspectral vegetation index [ 24 , 28 ]. Specifically, chlorophyll fluorescence kinetics provide insights into the dynamic changes in the light-absorbing and energy-dissipating functions of chlorophyll a under variable light conditions.…”

Section: Introductionmentioning

confidence: 99%

“…This method facilitates an in-depth analysis of the influence of cellular composition and arrangement on the optical properties of leaves, and improves understanding of the modulation of light across both standard and nonstandard leaves, as well as analysing the spectral and fluorescence curves on both adaxial and abaxial surfaces [ 9 , 11 , 14 , 29 ]. By integrating detailed microscopic analyses of cellular structures with advanced spectral techniques, we aim to enhance our understanding of the complex interplay between plant cell morphology and function [ 11 , 28 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Hyperspectral and Chlorophyll Fluorescence Analyses of Comparative Leaf Surfaces Reveal Cellular Influences on Leaf Optical Properties in Tradescantia Plants

Falcioni,

Antunes,

Berti de Oliveira

et al. 2024

Cells

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The differential effects of cellular and ultrastructural characteristics on the optical properties of adaxial and abaxial leaf surfaces in the genus Tradescantia highlight the intricate relationships between cellular arrangement and pigment distribution in the plant cells. We examined hyperspectral and chlorophyll a fluorescence (ChlF) kinetics using spectroradiometers and optical and electron microscopy techniques. The leaves were analysed for their spectral properties and cellular makeup. The biochemical compounds were measured and correlated with the biophysical and ultrastructural features. The main findings showed that the top and bottom leaf surfaces had different amounts and patterns of pigments, especially anthocyanins, flavonoids, total phenolics, chlorophyll-carotenoids, and cell and organelle structures, as revealed by the hyperspectral vegetation index (HVI). These differences were further elucidated by the correlation coefficients, which influence the optical signatures of the leaves. Additionally, ChlF kinetics varied between leaf surfaces, correlating with VIS-NIR-SWIR bands through distinct cellular structures and pigment concentrations in the hypodermis cells. We confirmed that the unique optical properties of each leaf surface arise not only from pigmentation but also from complex cellular arrangements and structural adaptations. Some of the factors that affect how leaves reflect light are the arrangement of chloroplasts, thylakoid membranes, vacuoles, and the relative size of the cells themselves. These findings improve our knowledge of the biophysical and biochemical reasons for leaf optical diversity, and indicate possible implications for photosynthetic efficiency and stress adaptation under different environmental conditions in the mesophyll cells of Tradescantia plants.

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Section: Adaptations To Cell and Tissue Structures From Spectral Datasupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hyperspectral and Chlorophyll Fluorescence Analyses of Comparative Leaf Surfaces Reveal Cellular Influences on Leaf Optical Properties in Tradescantia Plants

Falcioni,

Antunes,

Berti de Oliveira

et al. 2024

Cells

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Doctor Blade Casting of Thin Films Containing Different Concentrated Endemic Plant Extracts: Determination of Structure and Optical Properties

Fırat,

Emsen,

Sarsılmaz

et al. 2024

Karamanoğlu Mehmetbey Üniversitesi Mühendislik Ve Doğa Bilimleri Dergisi

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This study provides a thorough properties of the optical analysis of the thin films which produced from Astragalus tokatensis Fisch., Helichrysum noeanum Boiss. and Stachys huber-morathii R. Bhattacharjee extracts. Methanol extracts of plants were obtained via Soxhlet extractor. The highest extract yield (10.10%) was determined in H. noeanum. Doctor blade coating method is used to make thin film layer on glass substrate. The optical behavior of the deposited films is tested by means of he UV-vis-near IR absorbance and transmittance characterization. It is found that the maximum transmittance spectra reaches nearly to a value of 90 % for A. tokatensis sample. Significantly, all the samples display same optical absorbace spectra behavior. Energy band gaps of the films are presented based on Tauc relation and were found to be in the range between 3.68-3.81 eV. Besides, the analysis of functional groups available in the materials is broadly studied by Fourier transform infrared (FT-IR) spectroscopy. FT-IR measurement also confirms that all produced films have carbohydrate pattern. These findings demonstrate a cost-efficient approach for the production of thin films with plant extraction, and open a new perspective on the potential applications of optoelectronic devices.

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A fluorescence spectroscopic study of light transmission and adaxial-abaxial distribution of emitting compounds in leaves of Christmas star (Euphorbia pulcherrima)

Cited by 2 publications

References 23 publications

Hyperspectral and Chlorophyll Fluorescence Analyses of Comparative Leaf Surfaces Reveal Cellular Influences on Leaf Optical Properties in Tradescantia Plants

Hyperspectral and Chlorophyll Fluorescence Analyses of Comparative Leaf Surfaces Reveal Cellular Influences on Leaf Optical Properties in Tradescantia Plants

Doctor Blade Casting of Thin Films Containing Different Concentrated Endemic Plant Extracts: Determination of Structure and Optical Properties

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