A fluorescent aptasensor for Pb2+ detection based on gold nanoflowers and RecJf exonuclease-induced signal amplification

Electrochemical aptasensor based on DNA-templated copper nanoparticles and RecJf exonuclease-assisted target recycling for lipopolysaccharide detection

“…RecJf exonuclease is also responsible for catalyzing the stepwise removal of the aptamer-target complex DNA strand beginning from the 5′-terminus. 32 Owing to its sequence independence, great efficiency, and high accessibility, RecJf is used in several nucleic acid-based sensors for regenerating analytes and thereby enhancing detection sensitivity. 33 For instance, Wang et al prepared an electrochemical aptasensor based on RecJf exonuclease-assisted signal amplification and the PEI-g-C 3 N 4 /Au@Ni-CoHNB nanocomposites for the detection of di(2-ethylhexyl) phthalate (DEHP).…”

An efficient electrochemical biosensor for the detection of heavy metal lead in food based on magnetic separation strategy and Y-DNA structure

“…2 Lead (Pb) is a common toxic substance that can persist in the environment for a long time, causing great harm to water resources, soil and organisms. 3 Toxicological studies have shown that lead ions (Pb 2+ ) can accumulate in the body through skin contact, inhalation and diet and cannot be metabolized and degraded naturally. 4 It causes organ damage, neurological and cardiovascular system disorders, and even cancer and deformities.…”

RecJf exonuclease-assisted signal amplification for the sensitive detection of di(2-ethylhexyl)phthalate by a Pd@Au NB labeled electrochemical aptasensor based on Au@Ni-CoHNB/PEI-g-C3N4

“…The RecJf exonuclease has been applied to the development of many ultrasensitive aptasensors in several studies. Liu et al 45 introduced a fluorescent aptasensor based on the RecJf exonuclease-aided target recycling to amplify fluorescence signals for Pb 2+ detection. Tan et al 46 reported the use of an immobilization-free electrochemical aptasensor with RecJf exonuclease catalyzed target recycling amplification for the ultra-sensitive detection of ochratoxins.…”