A fluorescent probe for investigating metabolic stability of active transplatin analogues

Kolanowski, Jacek L.; Dawson, Lucy J.; Mitchell, Linda; Lim, Zelong; Graziotto, Marcus E.; Filipek, Wojciech K.; Hambley, Trevor W.; New, Elizabeth J.

doi:10.1016/j.snb.2017.09.084

Cited by 18 publications

(12 citation statements)

References 25 publications

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“…[28] Because of their structural similarity to fluoresceins,f unctionalisation of rhodamines usually proceeds in as imilar manner.F urthermore,r hodamines can be functionalised at the C2 position via addition reactions. [70] Both rhodamines and fluoresceins exist in equilibrium between their non-fluorescent lactone "closed" form, and their fluorescent quinoid "open" form ( Figure 5), ap rocess commonly exploited in probe design. [71,72] Thecationic nature of the open rhodamine form can lead to mitochondrial localisation, and this has been previously exploited in the design of mitochondrially-targeted sensors.…”

Section: Dual-functionalised Xanthene-based Probesmentioning

confidence: 99%

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Dual‐Functionalisation of Fluorophores for the Preparation of Targeted and Selective Probes

2020

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A key current challenge in biological research is the elucidation of the that roles chemicals and chemical reactions play in cellular function and dysfunction. Of the available cellular imaging techniques, fluorescence imaging offers a balance between sensitivity and resolution, enabling the cost‐effective and rapid visualisation of model biological systems. Importantly, the use of responsive fluorescent probes in conjunction with ever‐advancing microscopy and flow cytometry techniques enables the visualisation, with high spatiotemporal resolution, of both specific chemical species and chemical reactions in living cells. Ideal responsive fluorescent probes are those that contain a fluorophore tethered to both a sensing unit, to ensure selectivity of response, and a targeting group, to control the sub‐cellular localisation of the probe. To date, probes that are both targeted and selective are relatively rare and most localised probes are discovered serendipitously rather than by design. A challenge in this field is therefore the identification of suitable fluorophore scaffolds that can be readily attached to both sensing and targeting groups. Here we review current strategies for dual‐functionalisation of fluorophores, highlighting key examples of targeted, responsive probes.

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Section: Dual-functionalised Xanthene-based Probesmentioning

confidence: 99%

“…Because of their structural similarity to fluoresceins, functionalisation of rhodamines usually proceeds in a similar manner. Furthermore, rhodamines can be functionalised at the C2 position via addition reactions [70] …”

Section: Dual‐functionalised Xanthene‐based Probesmentioning

confidence: 99%

Dual‐Functionalisation of Fluorophores for the Preparation of Targeted and Selective Probes

2020

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“…Importantly, these techniques lack the ability to differentiate between metallic silver, precipitated silver salts and complexes and free Ag(I). In general, for metalsensing applications, optical detection approaches using selective sensors have been shown to be simple and costeffective, with sensitivity to oxidation state and coordination environment [24,25]. Fluorescent probes should therefore provide an avenue for efficient monitoring of biologically relevant Ag(I) species at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…Each tube was filled with 50 ml of deionized water (pH 7 + 0.5) or PBS saline solution (0.01 M, pH 7.4) and placed in a circulating water bath at 208C or 378C. At various time points(1,6,24,48, 72, 96…”

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confidence: 99%

A reversible fluorescent probe for monitoring Ag(I) ions

Lim

Smith

Kolanowski

et al. 2018

J. R. Soc. Interface.

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Silver-containing nanomaterials are of interest for their antibiotic properties, for a wide range of applications from medicine to consumer products. However, much remains to be learnt about the degradation of such materials and their effects on human health. While most analyses involve measurement of total silver levels, it is important also to be able to measure concentrations of active free Ag(I) ions. We report here the preparation of a coumarin-based probe, thiocoumarin silver sensor 1 (), that responds reversibly to the addition of silver ions through the appearance of a new fluorescence emission peak at 565 nm. Importantly, this peak is not observed in the presence of Hg(II), a common interferent in Ag(I) sensing. To establish the utility of this sensor, we prepared silver-doped phosphate glasses with demonstrated bactericidal properties, and observed the Ag(I) release from these glasses in solutions of different ionic strength. is therefore a useful tool for the study of the environmental and medical effects of silver-containing materials.

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“…20 Our research group has previously reported a fluorescein-based sensor for monofunctional platinum complexes 24 and a rhodamine-based sensor for aromatic transplatin analogues. 25 These have primarily been developed with the aim of improving future drug design, by providing an understanding of the cellular activity of platinum drugs, and are particularly useful for imaging studies of cell culture models. An important limitation to consider with selective sensors is the narrow range of analytes they are able to detect, and with the growing number of platinum complexes being designed and tested, achieving selectivity for each complex is an ongoing challenge.…”

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A Versatile Fluorescent Sensor Array for Platinum Anticancer Drug Detection in Biological Fluids

et al. 2021

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Platinum complexes remain frontline anticancer therapies, even after 50 years of usage in clinical applications. However, there is still a lack of methodology to robustly detect and quantify these complexes in biological fluids. We report here a fluorescent sensor array comprising six sensors that demonstrates progress toward the detection of platinum levels in chemotherapy patients. Linear discriminant analysis was performed to examine each multidimensional data set, and the array was able to discriminate platinum from other biologically relevant metals and heavy metals and separately able to differentiate and identify platinum complexes with different coordination environments with 100% accuracy. Finally, the array showed sensitivity to various cisplatin and oxaliplatin concentrations in human plasma and was able to discriminate between a cohort of 27 cancer patients at different stages of platinum treatment. We envisage that our array system could lead to a better understanding of blood platinum concentrations of chemotherapy patients and could inform the modification of dosage regimes to minimize dose-limiting side effects.

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A fluorescent probe for investigating metabolic stability of active transplatin analogues

Cited by 18 publications

References 25 publications

Dual‐Functionalisation of Fluorophores for the Preparation of Targeted and Selective Probes

Dual‐Functionalisation of Fluorophores for the Preparation of Targeted and Selective Probes

A reversible fluorescent probe for monitoring Ag(I) ions

A Versatile Fluorescent Sensor Array for Platinum Anticancer Drug Detection in Biological Fluids

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