A fluorescent probe, toluidinyl-naphthalene-sulfonate, specific for the β structure of poly-l-lysine

Binding interaction of differently charged fluorescent probes with egg yolk phosphatidylcholine and the effect of β-cyclodextrin on the lipid–probe complexes: A fluorometric investigation

“…The anionic fluorophore, 8-anilino-1-naphthalene sulfonate (ANS) is a fluorescent probe, which shows a strong dependence of its fluorescence quantum yield (and also the wavelength of the emission maximum) on the microenvironment around the molecule. Thus, it serves as an excellent probe for sensing the polarity of the biomimicking and biological environments [19][20][21][22][23][24][25][26][27]. ANS is poorly fluorescent in water, but it fluoresces strongly when trapped in the less polar region of confined environments [23,24,27].…”

“…Thus, it serves as an excellent probe for sensing the polarity of the biomimicking and biological environments [19][20][21][22][23][24][25][26][27]. ANS is poorly fluorescent in water, but it fluoresces strongly when trapped in the less polar region of confined environments [23,24,27]. Nile red (NR), the neutral dye from the phenoxazone family, has been comprehensively used as a staining agent in tissues [28].…”

Induction of “Pore” Formation in Plant Cell Membranes by Toluene

“…Aliquots from each vial, as well as the root tissue itself at the conclusion of the experiment, were Plasmalemma leakage may also be made apparent under the fluorescent microscope by following the entry into the cell of a compound such as ANS or TNS which does not permeate through undamaged membranes and which fluoresces when dissolved in the hydrophobic regions of certain proteins (9,13 Methods"). It will be seen that the half-times were not greatly altered by the toluene treatment: the most marked effect of the latter was on the relative volumes.…”

Fluorescent Protein Conjugates

“…Franke (248) has made equilibrium measurements of the binding of polycyclic aromatic hydrocarbons to human serum albumin. Lynn and Fasman (140) showed that binding of TNS occurs to the beta form of poly-L-Iysine, but not to the alpha form or to the random-coil form. Stryer (186) has given a brief review of the properties of the so-called fluorescent probes such as dansamide, ANS, and TNS and the use of these dyes in obtaining information about proteins concerning polarity, interatomic distances, rotational motion, and flexibility.…”

“…The correlation between fluorescence enhancement and the Kosower Z values mentioned above(131) would seem to supply a more preferable variable than the dielectric constant with which to discuss enhancement effects. A correlation with helical structure of polypeptides has been studied by Lynn and Fasman, (140) who found that only the beta helix but not the alpha-helical form or the random coil form of PolY-L-lysine produced a fluorescent enhancement of toluidinylnaphthalenesulfonates. From this result it was inferred that only the beta helix binds this dye.…”