A fluorogenic BODIPY molecular rotor as an apoptosis marker

Ashokkumar, Pichandi; Ashoka, Anila Hoskere; Collot, Mayeul; Das, Amitava; Klymchenko, Andrey S.

doi:10.1039/c9cc03242h

Cited by 55 publications

(46 citation statements)

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“…Tracking of individual viral proteins during viral disassembly, uncoating, or nuclear events should allow precise determination of the viral core remodeling mechanism. Likewise, additional information can be provided by using functional fluorescent probes capable of detecting environmental properties such as viscosity, polarity, membrane tension or pH [ 109 , 110 , 111 , 112 , 113 ]. Imaging of these probes conjugated to viral proteins will provide new original data on the physico-chemical characteristics of the intraviral environment and will highlight the modifications of the viral complexes linked to their remodeling during the early steps of infection.…”

Section: Discussionmentioning

confidence: 99%

Imaging Viral Infection by Fluorescence Microscopy: Focus on HIV-1 Early Stage

Mukherjee

Boutant²,

Réal³

et al. 2021

Viruses

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During the last two decades, progresses in bioimaging and the development of various strategies to fluorescently label the viral components opened a wide range of possibilities to visualize the early phase of Human Immunodeficiency Virus 1 (HIV-1) life cycle directly in infected cells. After fusion of the viral envelope with the cell membrane, the viral core is released into the cytoplasm and the viral RNA (vRNA) is retro-transcribed into DNA by the reverse transcriptase. During this process, the RNA-based viral complex transforms into a pre-integration complex (PIC), composed of the viral genomic DNA (vDNA) coated with viral and host cellular proteins. The protective capsid shell disassembles during a process called uncoating. The viral genome is transported into the cell nucleus and integrates into the host cell chromatin. Unlike biochemical approaches that provide global data about the whole population of viral particles, imaging techniques enable following individual viruses on a single particle level. In this context, quantitative microscopy has brought original data shedding light on the dynamics of the viral entry into the host cell, the cytoplasmic transport, the nuclear import, and the selection of the integration site. In parallel, multi-color imaging studies have elucidated the mechanism of action of host cell factors implicated in HIV-1 viral cycle progression. In this review, we describe the labeling strategies used for HIV-1 fluorescence imaging and report on the main advancements that imaging studies have brought in the understanding of the infection mechanisms from the viral entry into the host cell until the provirus integration step.

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Section: Discussionmentioning

confidence: 99%

Imaging Viral Infection by Fluorescence Microscopy: Focus on HIV-1 Early Stage

Mukherjee

Boutant²,

Réal³

et al. 2021

Viruses

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“…On the other hand, optical imaging, in particular fluorescence-guided surgery [34][35][36][37], allows the surgeons to visualize the normal and diseased tissues in real-time. As biological cells and tissues are poorly fluorescent, this technique requires specially designed fluorescent probes [38][39][40][41][42][43][44]. Fluorescent probes that operate in the near-infrared (NIR) region between 650-900 nm gained increasing attention, because biological tissues have low autofluorescence and negligible absorption coefficients in the NIR region [37,[45][46][47][48].…”

Section: Introductionmentioning

confidence: 99%

Near-infrared fluorescent coatings of medical devices for image-guided surgery

et al. 2020

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Rapidly expanding field of image-guided surgery needs new materials for near-infrared imaging with deep tissue penetration. Here, we introduce near-infrared coating of equipment (NICE) for image-guided surgery based on a series of lipophilic cyanine-7.5 dyes with bulky hydrophobic counterions and a biocompatible polymer, poly(methyl methacrylate). The NICE material exhibits superior brightness (15-20-fold higher) and photostability compared to fluorescent coatings based on commonly used indocyanine green (ICG). It can be deposited on different surfaces and devices, such as steel and gold fiducials, silicone and PVC catheters, polymeric surgical sutures and gauzes. Such coated medical devices show excellent stability in air and buffer for 150 days. Accelerated ageing revealed their shelf-life of 3 years. They are also stable in serum-containing media, whereas ICG-based coating shows rapid dye leakage. NICE is compatible with standard sterilization protocols based on ethylene oxide and vapor. Moreover, our coating material is biocompatible, where cultured cells spread effectively without signs of cytotoxicity. Ex vivo studies suggest that NICE on fiducials can be visualized as deep as 0.5 cm, and NICE on catheters enables their visualization inside ureters and esophagus. Finally, NICE on different medical devices has been validated for image-guided surgery in porcine and human cadaver models. Thus, the developed NIR coating material emerges as a powerful tool for a variety of medical applications.

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“…23,24 Second, molecular rotors, 25 which are poorly uorescent in aqueous media, but light up upon binding to the targets with highly viscous environment. 26,27 One should also mention aggregation-induced emission approach, where the nonemissive dyes light up because the target triggers dye aggregation. 28 Of particular interest are probes operating by aggregation-caused quenching (ACQ) mechanism.…”

Section: Introductionmentioning

confidence: 99%