A Full-Length cDNA Encoding 1-Aminocyclopropane-1-Carboxylate Synthase from Apple

Lay-Yee, Michael; Knighton, Michelle L.

doi:10.1104/pp.107.3.1017

Cited by 25 publications

(9 citation statements)

References 4 publications

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“…A small gene family of ACSs encoding different isoforms of the enzyme has been identified in Arabidopsis thaliana and other species (Van Der Straeten et al, 1990Liang et al, 1992;Lay-Yee and Knighton, 1995;Arteca and Arteca, 1999;The Arabidopsis Genome Initiative, 2000). The expression of the genes depends on both internal and external factors (Oetiker et al, 1997;Peck and Kende, 1998;Barry et al, 2000) and is considered tissue-specific (Liang et al, 1995;Tsuchisaka and Theologis, 2004).…”

Section: Introductionmentioning

confidence: 97%

Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition

Frankowski

Kęsy

Wojciechowski

et al. 2009

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 97%

Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition

Frankowski

Kęsy

Wojciechowski

et al. 2009

Journal of Plant Physiology

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“…In the first step, 1-aminocyclopropane-1-carboxylate (ACC) synthase converts SAM to ACC; then, ACC oxidase catalyzes the oxidative fragmentation of ACC to form ethylene (Yang and Hoffman 1984). Genes encoding both of these two enzymes have recently been cloned from a number of plant species (Sato and Theologis 1989;Van Der Straeten et al 1990;Nakajima et al 1990;Lay-Yee and Knighton 1995). It is clear that both genes are encoded by multigene families and are under developmental regulation (Kende 1993).…”

Section: Introductionmentioning

confidence: 98%

Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers

et al. 2003

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In Japanese pear (Pyrus pyrifolia Nakai), fruit storage potential is closely related to the amount of ethylene produced. We have developed a rapid and accurate method for analyzing genes involved in high ethylene production during fruit ripening in Japanese pear. This involves cleaved-amplified polymorphic sequences (CAPS) of two 1-aminocyclopropane-1-carboxylate (ACC) synthase genes (PPACS1 and PPACS2). Two CAPS markers (A for PPACS1 and B for PPACS2), associated with the amount of ethylene produced, were identified. Marker A was associated with high ethylene producers and marker B with moderate ethylene producers. The absence of these two markers enabled the identification of low ethylene producers. Using these markers, we have identified ethylene genotypes for 40 Japanese pear cultivars and two Chinese pear (P. bretschneideri) cultivars that are commercially important and used in breeding programs. Furthermore, we performed linkage analysis of these two genes in the F(2) population, which revealed that the recombination frequency between the two markers was 20.8 +/- 3.6%. This information is critical to the selection of parents and in breeding strategies to improve storage ability of Japanese pears.

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“…In recent years signi®cant progress has been made in our understanding of the biosynthetic pathway and the molecular regulation of ethylene production (Adams and Yang 1979;Kende 1993). Cloning and characterization of genes encoding the two key enzymes involved, ACC synthase and ACC oxidase, has been carried out in many species (Sato and Theologis 1989;Van Der Straeten et al 1990;Nakajima et al 1991;Lay-Yee and Knighton 1995). These reports have shown that ACC synthase and ACC oxidase are encoded by a multigene family whose various members are dierentially expressed in response to many factors (Olson et al 1991;Rottmann et al 1991;Lincoln et al 1993;Lasserre et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Identification of 1-aminocyclopropane-1-carboxylic acid synthase genes controlling the ethylene level of ripening fruit in Japanese pear (Pyrus pyrifolia Nakai)

Itai¹,

Kawata²,

Tanabe³

et al. 1999

Mol Gen Genet

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The shelf life of Japanese pear fruit is determined by its level of ethylene production. Relatively high levels of ethylene reduce storage potential and fruit quality. We have identified RFLP markers tightly linked to the locus that determines the rate of ethylene evolution in ripening fruit of the Japanese pear. The study was carried out using sequences of two types of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase genes (PPACS1 and pPPACS2) and a ACC oxidase gene (PPAOX1) as probes on 35 Japanese pear cultivars expressing different levels of ethylene (0.0 to approximately 300 microl/kg fresh weight/h) in ripening fruit. When total DNA was digested with HindIII and probed with pPPACS1, we identified a band of 2.8 kb which was specific to cultivars having very high ethylene levels (> or = 10 microl/kg f.w./h) during fruit ripening. The probe pPPACS2 identified a band of 0.8 kb specific to cultivars with moderate ethylene levels (0.5 microl/kg f.w./h-10 microl/kg f.w./h) during fruit ripening. The cultivars that produce high levels of ethylene possess at least one additional copy of pPPACS1 and those producing moderate levels of ethylene have at least one additional copy of pPPACS2. These results suggest that RFLP analysis with different ACC synthase genes could be useful for predicting the maximum ethylene level during fruit ripening in Japanese pear.

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A Full-Length cDNA Encoding 1-Aminocyclopropane-1-Carboxylate Synthase from Apple

Cited by 25 publications

References 4 publications

Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition

Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition

Rapid identification of 1-aminocyclopropane-1-carboxylate (ACC) synthase genotypes in cultivars of Japanese pear (Pyrus pyrifolia Nakai) using CAPS markers

Identification of 1-aminocyclopropane-1-carboxylic acid synthase genes controlling the ethylene level of ripening fruit in Japanese pear (Pyrus pyrifolia Nakai)

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