A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Lee, Bor-Shiunn; Lee, Chien-Chen; Lin, Hung-Pin; Shih, Wei-An; Hsieh, Wan-Ling; Lai, Chung-Sheng; Takeuchi, Yasuo; Chen, Yiwen

doi:10.1016/j.carbpol.2016.06.026

Cited by 45 publications

(30 citation statements)

References 59 publications

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“…21 In Groups 1 (PLGA-lovastatin) and 2 (MTA), it was noted that dystrophic calcification occurred at a similar or higher ratio in the observation period of 4 weeks compared to that in the observation period of 2 weeks. However, the results contradicted those of the control group.…”

mentioning

confidence: 92%

“…17,18,21 The concentrations of 1 and 2 μg/mL of free lovastatin were selected in this study because our previous study revealed that the ALP activity in cell cultures greatly decreased with an increase in the concentration of free lovastatin (2, 4, 8, and 16 μg/mL). 17 In this study, PLGAlovastatin nanoparticles with a median diameter of 174.8 nm and a 1:1 molar ratio of lactide to glycolide polymer were used as the carrier.…”

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confidence: 99%

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Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

Lin¹,

Tu²,

Hsieh³

et al. 2017

IJN

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Statin at appropriate concentrations has been shown to induce odontoblastic differentiation, dentinogenesis, and angiogenesis. However, using a carrier to control statin release might reduce toxicity and enhance its therapeutic effects. The aim of this study was to prepare poly( d , l -lactide- co -glycolide acid) (PLGA) nanoparticles that contain lovastatin for application in direct pulp capping. The PLGA–lovastatin particle size was determined using dynamic light scattering measurements and transmission electron microscopy. In addition, the release of lovastatin was quantified using a UV–Vis spectrophotometer. The cytotoxicity and alkaline phosphatase (ALP) activity of PLGA–lovastatin nanoparticles on human dental pulp cells were investigated. Moreover, a real-time polymerase chain reaction (PCR) assay, Western blot analysis, and an enzyme-linked immunosorbent assay (ELISA) were used to examine the osteogenesis gene and protein expression of dentin sialophosphoprotein (DSPP), dentin matrix acidic phosphoprotein 1 (DMP1), and osteocalcin (OCN). Finally, PLGA–lovastatin nanoparticles and mineral trioxide aggregate (MTA) were compared as direct pulp capping materials in Wistar rat teeth. The results showed that the median diameter of PLGA–lovastatin nanoparticles was 174.8 nm and the cumulative lovastatin release was 92% at the 44th day. PLGA–lovastatin nanoparticles demonstrated considerably a lower cytotoxicity than free lovastatin at 5, 9, and 13 days of culture. For ALP activity, the ALP amount of PLGA–lovastatin (100 μg/mL) was significantly higher than that of the other groups for 9 and 13 days of culture. The real-time PCR assay, Western blot analysis, and ELISA assay showed that PLGA–lovastatin (100 μg/mL) induced the highest mRNA and protein expression of DSPP, DMP1, and OCN in pulp cells. Histological evaluation of the animal studies revealed that MTA was superior to the PLGA–lovastatin in stimulating the formation of tubular dentin in an observation period of 2 weeks. However, in an observation period of 4 weeks, it was evident that the PLGA–lovastatin and MTA were competitive in the formation of tubular reparative dentin and a complete dentinal bridge.

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mentioning

confidence: 92%

mentioning

confidence: 99%

Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

Lin¹,

Tu²,

Hsieh³

et al. 2017

IJN

Self Cite

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“…Similarly, the biocompatibility of the adhesive should be taken seriously. Beatriz M et al evaluated the biological and mechanical performance of adhesive added with different concentration of EGCG and found that 0.5 and 1.0 wt% EGCG-doped adhesive system have less cytotoxicity (Lee, Lee, Lin, et al, 2016).…”

Section: Egcg In Dental Adhesivesmentioning

confidence: 99%

“…Due to the excellent bacteriostat, anti-fibrosis (Nakamuta et al, 2005) and osteogenesis effects (Glowacki & Mizuno, 2008;Miyata, Taira, & Noishiki, 1992) (Lee, Lee, Lin, et al, 2016).…”

Section: Egcg In Barrier Membranementioning

confidence: 99%

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

Liao

Tang

Chu

et al. 2020

J Biomedical Materials Res

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Because of excellent biocompatibility, antioxidant activity, and anti-caries ability, epigallocatechin-3-gallate (EGCG) has been widely studied in the treatment of oral diseases, such as periodontal disease, oral cancer, and dental caries. To reach the site of the lesion or achieve sustained release, play the role of anti-caries, anti-inflammatory, or to maintain or improve the physical properties of the modified material， EGCG need to be cross-linked or embedded with dental adhesives, barrier membranes, bone replacement materials, tissue regeneration materials, and antimicrobial anti-caries materials to better prevent or treat oral diseases. This article reviews the applications of EGCG in oral materials, involving various areas of the oral cavity, reveals their excellent potential, and sees shortcomings in these research to promote the better development of EGCG applications in oral materials such as oral repair materials, bone tissue engineering materials and antibacterial and anti-caries materials.

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“…In the past twenty years, resorbable membranes have been investigated and introduced to GTR or GBR techniques. In recent years, resorbable membranes using materials such as chitosan 11 , collagen 12 and gelatin/alginate 13 have gained a lot of attention in biomedical applications. However, these membranes possess some drawbacks due to their minimalmechanical strength and lake of physical stability.…”

Section: Introductionmentioning

confidence: 99%

Influence of Niobium Pentoxide Particulates on the Properties of Brushite/Gelatin/Alginate Membranes

Beherei

Shaltout

Mabrouk

et al. 2018

Journal of Pharmaceutical Sciences

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Novel nonporous membranes were prepared by impregnating brushite and niobium pentoxide (NbO) into a gelatin/alginate matrix. The physicochemical properties, morphology, and mechanical properties of the prepared membranes were characterized using X-ray diffractometer, FTIR spectroscopy, scanning electron microscopy, transmission electron microscopy, and universal testing machine, respectively. Swelling ability of the prepared membranes was determined in distilled water. The surfaces of the membranes were characterized by means of FTIR spectroscopy and scanning electron microscopy coupled with energy dispersive x-ray spectroscopy after submersion in simulated body fluid up to 15 days. Moreover, the calcium and phosphorus ion concentrations in the simulated body fluid were measured using an UV spectrophotometer. The in vitro drug release and the release mechanism of a model antibiotic, namely, ciprofloxacin (CFX), were tested in phosphate-buffered saline for 15 days. The antibacterial activities of the CFX-loaded membranes were tested against known microorganisms. The physicochemical properties, morphology, mechanical properties, and swelling ability of the prepared membranes were found to be dependent on the presence of NbO allowing control of their properties. For example, the NbO-loaded membranes exhibited a higher in vitro bioactivity and slower drug release compared to those of NbO-free membranes. The CFX-loaded membranes also exhibited an excellent inhibition zones against the selected microorganisms. Overall, the prepared membranes have been found to be very promising for use in bone substitute applications.

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A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Cited by 45 publications

References 59 publications

Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

Controlled release of lovastatin from poly(lactic-<em>co</em>-glycolic acid) nanoparticles for direct pulp capping in rat teeth

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

Influence of Niobium Pentoxide Particulates on the Properties of Brushite/Gelatin/Alginate Membranes

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