A functional polymorphism of inhibin alpha subunit at miR-181b-1-3p-binding site regulates proliferation and apoptosis of chicken ovarian granular cells

Cui, Zhifu; Shen, Xiaoxu; Zhang, Xianxian; Li, Fugui; Amevor, Felix Kwame; Zhu, Qing; Wang, Yan; Li, Diyan; Shu, Gang; Tian, Yaofu; Zhao, Xiaoling

doi:10.1007/s00441-020-03356-w

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…The upstream and downstream regions of genes are often rich in elements that influence transcription and post-transcription regulation, such as transcription factor binding sites, miRNA binding sites, and upstream open reading frames (uORFs) [28][29][30]. Prior research has linked polymorphisms in these regions to variations in gene expression and growth performance [31][32][33][34][35]. In our study, we screened the VNN1 flanking regions and identified 39 SNPs, with 23 linked to carcass traits and 8 reported for the first time.…”

Section: Discussionmentioning

confidence: 99%

VNN1 Gene Expression and Polymorphisms Associated with Chicken Carcass Traits

Zhang,

Fang,

et al. 2024

Animals

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This study aimed to investigate the association between hepatic VNN1 expression and carcass traits in Mahuang chickens as well as to identify polymorphisms in the upstream and downstream regions of VNN1 that could potentially be associated with these carcass traits. The study revealed that VNN1 expression levels in liver correlated with various carcass traits such as dressed weight, eviscerated weight, and abdominal fat weight. A total of 39 polymorphic sites were identified, among which 23 were found to be associated with 15 different carcass traits. These polymorphic sites were organized into three distinct haplotype blocks, with BLOCK2 and BLOCK3 being associated with various eviscerated weight percentages, thigh weight, breast muscle weight, wing weight, and other traits. The study underscores the significant role of VNN1 in influencing the carcass traits of Mahuang chickens and sheds light on the genetic foundations of these traits. The findings provide valuable insights that could inform breeding strategies aimed at optimizing traits relevant to market demands and slaughtering efficiency.

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Section: Discussionmentioning

confidence: 99%

VNN1 Gene Expression and Polymorphisms Associated with Chicken Carcass Traits

Zhang,

Fang,

et al. 2024

Animals

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“…Radiation therapy stimulates latent immune cells and is a well-known treatment for cancer, with more than 50% of newly diagnosed cancer patients with solid tumors receiving radiation as treatment. Its mechanism involves causing damage to the DNA backbone or the generation of ROS [16]. In addition, it has been reported that radiation influences the expression of cytokines in the body, such as TNF-alpha and interleukin-1 (IL-1) [33].…”

Section: Discussionmentioning

confidence: 99%

“…INHA, the gene encoding the inhibin alpha subunit, is involved in mammalian folliculogenesis. The downregulation of INHA caused an increase in apoptosis and induced ROS production via a mitochondrial pathway in the cell [16]. On the other hand, it is shown that normal cells continuously generate ROS as a result of electron leakage from mitochondria through the electron transport system.…”

Section: Introductionmentioning

confidence: 99%

Combined Therapy with microRNA-Expressing Salmonella and Irradiation in Melanoma

et al. 2021

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Anticancer treatment strategies using bacteria as a vector are currently expanding with the development of anticancer drugs. Here, we present a research strategy to develop anticancer drugs using bacteria that contain miRNAs. We also present a strategy for the development of novel bacterial anticancer drugs in combination with radiation. Salmonella strains expressing miRNA were produced by modifying the miRNA expression vector encoding INHA, a radiation-resistant gene developed previously. The anticancer effect of INHA was confirmed using skin cancer cell lines. We also tested a combination strategy comprising bacteria and radiation for its anticancer efficacy against radiation-resistant mouse melanoma to increase the efficacy of radiation therapy as a novel strategy. The recombinant strain was confirmed to promote effective cell death even when combined with radiation therapy, which exerts its cytotoxicity by enhancing reactive oxygen species production. Moreover, a combination of bacterial and radiation therapy enhanced radiotherapy efficacy. When combined with radiation therapy, bacterial therapy exhibited effective anti-cancer properties even when administered to animals harboring radiation-resistant tumors. This strategy may promote the secretion of cytokines in cells and more effectively reduce the number of bacteria remaining in the animal. Thus, this study may lead to the development of a strategy to improve the effectiveness of radiation therapy using Salmonella expressing cancer-specific miRNA for intractable cancers such as those resistant to radiation.

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“…Hepatocyte apoptosis was detected using Flow Cytometry (CytoFLEX, Beckman Coulter, CA, USA) and Kaluza 2.1 software. Hepatocytes were washed with PBS and the concentration was adjusted to 1 × 10 6 cells/mL, and determination was performed as previously described ( Cui et al, 2021 ). DCFH-DA (2, 7-Dichlorofluorescin; Sigma) was added to hepatocytes at a final concentration of 10 μM for 20 min at room temperature, and then the hepatocyte Reactive Oxygen Species ( ROS ) were analyzed using a Shimadzu RF-3501 fluorescence spectrophotometer at an excitation wavelength of 488 nm and an emission wavelength of 525 nm.…”

Section: Methodsmentioning

confidence: 99%