A functionalized surface modification with vanadium nanoparticles of various valences against implant-associated bloodstream infection

Abstract

Bloodstream infection, especially with implants involved, is an often life-threatening condition with high mortality rates, imposing a heavy burden on patients and medical systems. Herein, we firstly deposited homogeneous vanadium metal, V 2 O 3 , VO 2 , and V 2 O 5 nanofilms on quartz glass by magnetron sputtering. Using these platforms, we further investigated the potential antimicrobial efficiency of these nano-VO x films and the interactions of human erythrocytes and bacteria (methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa ) with our samples in a novel cell–bacteria coculture model. It was demonstrated that these nano-VO x precipitated favorable antibacterial activity on both bacteria, especially on S. aureus , and this effect increased with higher vanadium valence. A possible mechanism accountable for these results might be elevated levels of vanadium-induced intracellular reactive oxygen species. More importantly, based on hemolysis assays, our nano-VO x films were found to be able to kill prokaryotic cells but were not toxic to mammalian cells, holding the potential for the prevention of implant-related hematogenous infections. As far as we know, this is the first report wherein such nano-VO x films have assisted human erythrocytes to combat bacteria in a valence-dependent manner. Additionally, vanadium ions were released from these nano-VO x films in a sustained manner, and low-valence films possessed better biocompatibility with human fibroblasts. This work may provide new insights for biomedical applications of inorganic vanadium compounds and attract growing attention in this field. From the perspective of surface modification and functionalization, this study holds promise to avail the prophylaxis of bloodstream infections involving implantable biomedical devices.

Antimicrobial Nanostructured Coatings: A Gas Phase Deposition and Magnetron Sputtering Perspective

“…Many different routes have been explored to synthesize NMs and NPs, from deposition of polymeric films [19,21,45,[49][50][51] with incorporated bactericidal agents [21,[52][53][54][55], or metallic or oxide films [2,21,56,57], to metal decoration of nanostructures [58][59][60]. The strategies are based on wet synthesis (spin-coating [61], sol-gel [57,61,62]), photochemical [52], biological [63][64][65], biotechnological [24] and physical methods (laser ablation [66], magnetron sputtering (MS) [2,44,[67][68][69], gas phase beams [70,71]). Surprisingly, the literature available on nanostructured coatings obtained by physical methods is scarce.…”

Aplicações Clínicas Dos Glicopeptídeos Nas Unidades De Terapia Intensivas

“…Nesta perspectiva é interessante a busca por novas estratégias, a necessidade de desenvolver alternativas microbicidas aumentaram para resolver as limitações dos medicamentos convencionais. Logo, os materiais baseados em nanopartículas surgiram como uma nova classe de agentes para a terapia antimicrobiana (WANG et al, 2017). A nanotecnologia tem muitas aplicações interessantes como, na área da agricultura, tecnológica, ambiental e saúde.…”

Application of Nanoparticle Technologies in the Combat against Anti-Microbial Resistance

“…Since the Gram-negative bacterial cell surface is mostly negatively charged [ 74 ] this will dictate how nanoparticles will interact. Many metal nanoparticles are cationic thus, due to such opposite charges the zeta potential of nanoparticles will be vital to the degree to which nanoparticles will be bound to the bacterial cell wall [ 75 ]. Halder and colleagues measured the zeta potentials of both S. aureus and E. coli and found the zeta potential values for their surface charge to be −35.6 mV and −44.2mV, respectively [ 69 ].…”