A Fusarium graminearum strain-comparative proteomic approach identifies regulatory changes triggered by agmatine

Pasquali, Matias; Serchi, Tommaso; Cocco, Emmanuelle; Leclercq, Céline C.; Planchon, Sébastien; Guignard, Cédric; Renaut, Jenny; Hoffmann, Lucien

doi:10.1016/j.jprot.2015.11.010

Cited by 8 publications

(14 citation statements)

References 68 publications

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“…The effect of 2 different media containing agmatine or glutamic acid on toxin induction and growth in a total of nineteen strains belonging to the F. graminearum ( n = 7) , F. culmorum ( n = 8) and F. poae ( n = 4) species was evaluated. With our species-comparative study, we confirmed that on average the mechanism of triggering toxin production by agmatine is confirmed in the 3 species but that the mechanism is also significantly influenced by strain diversity ( Pasquali et al, 2015 ). The identification of isolates that do not respond to agmatine may help decipher the pathways leading to specific agmatine regulation in the fungus.…”

Section: Discussionsupporting

confidence: 65%

The effect of agmatine on trichothecene type B and zearalenone production inFusarium graminearum,F. culmorumandF. poae

Pasquali

Cocco

Guignard

et al. 2016

PeerJ

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Agmatine and other putrescines are known for being strong inducers of deoxynivalenol (DON) production in Fusarium graminearum. Other important species produce DON and/or other trichothecene type B toxins (3 acetylated DON, 15 acetylated DON, Fusarenon-X, Nivalenol), such as F. culmorum and F. poae. In order to verify whether the mechanism of the regulation of trichothecene type B induction by agmatine is shared by different species of Fusarium, we tested the hypothesis on 19 strains belonging to 3 Fusarium species (F. graminearum, F. culmorum, F. poae) with diverse genetic chemotypes (3ADON, 15ADON, NIV) by measuring trichothecene B toxins such as DON, NIV, Fusarenon-X, 3ADON and 15ADON. Moreover, we tested whether other toxins like zearalenone were also boosted by agmatine. The trichothecene type B boosting effect was observed in the majority of strains (13 out of 19) in all the three species. Representative strains from all three genetic chemotypes were able to boost toxin production after agmatine treatment. We identified the non-responding strains to the agmatine stimulus, which may contribute to deciphering the regulatory mechanisms that link toxin production to agmatine (and, more generally, polyamines).

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Section: Discussionsupporting

confidence: 65%

The effect of agmatine on trichothecene type B and zearalenone production inFusarium graminearum,F. culmorumandF. poae

Pasquali

Cocco

Guignard

et al. 2016

PeerJ

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“…Our study identifies peculiarities of each strain that can be exploited for generating complex consortia able to effectively limit the disease [ 58 , 59 ]. Hopefully, future integration of omics analyses of the biocontrol strains may lead to the identification of effective molecules able to block fungal growth and modulate toxin biosynthesis [ 60 ] as well as to the understanding of mechanisms that modulate growth by sensing the fungal neighbor. A promising approach to developing novel targeted strategies to limit the damage of toxin accumulation in food and feed can come from the integration of this knowledge [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR

Colombo

Kunova

Gardana

et al. 2020

Toxins

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Streptomyces spp. can be exploited as biocontrol agents (BCAs) against plant pathogens such as Fusarium graminearum, the main causal agent of Fusarium head blight (FHB) and against the contamination of grains with deoxynivalenol (DON). In the present research, four Streptomyces strains active against F. graminearum in dual plate assays were characterized for their ability to colonize detached wheat grains in the presence of F. graminearum and to limit DON production. The pathogen and BCA abundance were assessed by a quantitative real-time PCR, while DON production was assessed by HPLC quantification and compared to ergosterol to correlate the toxin production to the amount of fungal mycelium. Fungal growth and mycotoxin production were assessed with both co-inoculation and late inoculation of the BCAs in vitro (three days post-Fusarium inoculation) to test the interaction between the fungus and the bacteria. The level of inhibition of the pathogen and the toxin production were strain-specific. Overall, a higher level of DON inhibition (up to 99%) and a strong reduction in fungal biomass (up to 71%) were achieved when streptomycetes were co-inoculated with the fungus. This research enabled studying the antifungal efficacy of the four Streptomyces strains and monitoring their development in DON-inducing conditions.

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“…Up to now, a relatively large set of transcriptomic and proteomic studies on the FGSC have been carried out, but most have focused on the identification of pathogenicity-associated genes, such as virulence proteins and effector-coding genes [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. The effect of FGSC strains on trichothecene production diversity according to transcriptomic or proteomic approaches has seldomly been explored [ 44 , 45 , 46 ]. The reported transcriptomic and proteomic studies on trichothecene production have all focused on the conditions conducive to mycotoxin production [ 44 , 45 , 46 ], while the molecular mechanisms of trichothecene biosynthesis in FGSC strains under normal or non-inducing conditions are still unclear.…”

Section: Introductionmentioning

confidence: 99%

“…The effect of FGSC strains on trichothecene production diversity according to transcriptomic or proteomic approaches has seldomly been explored [ 44 , 45 , 46 ]. The reported transcriptomic and proteomic studies on trichothecene production have all focused on the conditions conducive to mycotoxin production [ 44 , 45 , 46 ], while the molecular mechanisms of trichothecene biosynthesis in FGSC strains under normal or non-inducing conditions are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Identification of Candidate Genes Associated with Trichothecene Biosynthesis in Fusarium graminearum Species Complex Combined with Transcriptomic and Proteomic Analysis

et al. 2022

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The Fusarium graminearum species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in Fusarium strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix Fusarium GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by FGSG_01403, was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of FGSG_01403 resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the Fusarium graminearum species complex.

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A Fusarium graminearum strain-comparative proteomic approach identifies regulatory changes triggered by agmatine

Cited by 8 publications

References 68 publications

The effect of agmatine on trichothecene type B and zearalenone production inFusarium graminearum,F. culmorumandF. poae

The effect of agmatine on trichothecene type B and zearalenone production inFusarium graminearum,F. culmorumandF. poae

Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR

Identification of Candidate Genes Associated with Trichothecene Biosynthesis in Fusarium graminearum Species Complex Combined with Transcriptomic and Proteomic Analysis

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