Investigating Useful Properties of Four Streptomyces Strains Active against Fusarium graminearum Growth and Deoxynivalenol Production on Wheat Grains by qPCR

Abstract

Streptomyces spp. can be exploited as biocontrol agents (BCAs) against plant pathogens such as Fusarium graminearum, the main causal agent of Fusarium head blight (FHB) and against the contamination of grains with deoxynivalenol (DON). In the present research, four Streptomyces strains active against F. graminearum in dual plate assays were characterized for their ability to colonize detached wheat grains in the presence of F. graminearum and to limit DON production. The pathogen and BCA abundance were assessed by a quantitative real-time PCR, while DON production was assessed by HPLC quantification and compared to ergosterol to correlate the toxin production to the amount of fungal mycelium. Fungal growth and mycotoxin production were assessed with both co-inoculation and late inoculation of the BCAs in vitro (three days post-Fusarium inoculation) to test the interaction between the fungus and the bacteria. The level of inhibition of the pathogen and the toxin production were strain-specific. Overall, a higher level of DON inhibition (up to 99%) and a strong reduction in fungal biomass (up to 71%) were achieved when streptomycetes were co-inoculated with the fungus. This research enabled studying the antifungal efficacy of the four Streptomyces strains and monitoring their development in DON-inducing conditions.

Symptoms and pathogens diversity of Corn Fusarium sheath rot in Sichuan Province, China

“…In addition, the frequency of F. graminearum isolated from ears ranged from 30 to 71% with an average of 57%, and from stalks ranged from 43 to 81%, with the average of 65% 39 . Although the isolation frequency of F. graminearum from CFSR was only 3.65% in our present study, the potential risk of mycotoxins produced by F. graminearum to human health cannot be ignored 40 .…”

Fusarium musae from Diseased Bananas and Human Patients: Susceptibility to Fungicides Used in Clinical and Agricultural Settings

“…DNA was extracted from lyophilised mycelia following the protocol (200 microL) of the DNeasy Mericon food Kit (Qiagen, Germantown, MD, USA) [ 24 ]. Lyophilised mycelia (200 mg) were weighted and placed in a 2 mL microcentrifuge tube in presence of 1 mL food lysis buffer (provided by the kit) and 2.5 μL Proteinase K solution (provided by the kit).…”

Streptomyces Strains Promote Plant Growth and Induce Resistance Against Fusarium verticillioides via Transient Regulation of Auxin Signaling and Archetypal Defense Pathways in Maize Plants

“…Several studies have shown an effective biocontrol potential of Streptomyces spp. in suppression of the growth of F. graminearum , Aspergillus flavus , and their mycotoxin production ( Boukaew et al, 2020 ; Colombo et al, 2020 ).…”