A gas chromatography–mass spectrometry based study on serum metabolomics in rats chronically poisoned with hydrogen sulfide

Deng, Mingjie; Zhang, Meiling; Huang, Xueli; Ma, Jianshe; Hu, Lufeng; Lin, Gen‐Min; Wang, Xianqin

doi:10.1016/j.jflm.2015.02.014

Cited by 21 publications

(8 citation statements)

References 29 publications

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“…Metabolomics is a newly emerging omics approach to the investigation of metabolic phenotype changes induced by environmental or endogenous factors (Deng et al, 2015a, b; Patti, Yanes, & Siuzdak, ; Wang et al, 2015a, b; Wen et al, ; Wen et al, 2016a, b). The identification of endogenous compounds that can be used as metabolic biomarkers of alprazolam abuse would represent an alternative approach of significant importance to detect hidden effects.…”

Section: Resultsmentioning

confidence: 99%

Effect of alprazolam on rat serum metabolic profiles

Lin²,

Chen

et al. 2017

Biomedical Chromatography

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We developed a serum metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of alprazolam in rats. The GC-MS with HP-5MS (0.25 μm × 30 m × 0.25 mm) mass was conducted in electron impact ionization (EI) mode with electron energy of 70 eV, and full-scan mode with m/z 50-550. The rats were randomly divided to four groups, three alprazolam-treated groups and a control group. The alprazolam-treated rats were given 5, 10 or 20 mg/kg (low, medium, high) of alprazolam by intragastric administration each day for 14 days. The serum samples were corrected on the seventh and fourteenth days for metabolomic study. The blood was collected for biochemical tests. Then liver and brain were rapidly isolated and immersed for pathological study. Compared with the control group, on the seventh and fourteen days, the levels of d-glucose, 9,12-octadecadienoic acid, butanoic acid, l-proline, d-mannose and malic acid had changed, indicating that alprazolam induced energy metabolism, fatty acid metabolism and amino acid metabolism perturbations in rats. There was no significant difference for alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea and uric acid between controls and alprazolam groups. According to the pathological results, alprazolam is not hepatotoxic. Metabolomics could distinguish different alprazolam doses in rats.

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Section: Resultsmentioning

confidence: 99%

Effect of alprazolam on rat serum metabolic profiles

Lin²,

Chen

et al. 2017

Biomedical Chromatography

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“…There are currently no specific direct tests that can be used to diagnose poisoned patients. Deng et al applied a GC/MS‐based metabonomics approach to assess the metabolite changes in serum from rats following chronic hydrogen sulfide poisoning. They observed impaired metabolic pathways including lipid metabolism, energy metabolism, and amino acid metabolism in rat serum: decreased levels of amino acids, including alanine, valine, serine, threonine, and proline and elevated levels of galactose, citrate, glucose, and mannose indicate impairment in the carbohydrate metabolism.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Role of Toxicological Data in Discriminating Between H₂S Femoral Blood Concentration Secondary to Lethal poisoning and Endogenous H₂S Putrefactive Production

Barbera

Montana

Indorato

et al. 2016

Journal of Forensic Sciences

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Hydrogen sulfide is a colorless gas and has a strong odor of rotten eggs. It is absorbed by the upper respiratory tract mucosa, and it causes histotoxic hypoxemia and respiratory depression by exerting an inhibitory effect on cytochrome oxidase. To evaluate the role of toxicological data in distinguishing between the H S blood concentration secondary to lethal poisoning and the endogenous H S produced during putrefaction, we compared the postmortem H S concentrations of six fatal H S poisoning cases (8.7-28.6 mg/L) with the postmortem concentrations of endogenous H S of 12 subjects who died from other causes (traffic-related deaths) (2.2-32.7 mg/L). These results will be of interest to the forensic community as it underlines the importance of considering circumstantial evidence along with the toxicological and pathological findings in the identification of H S lethal poisoning.

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“…Dextromethorphan, as an over-the-counter medicine, is often rationalized by its users as being safer, easier to obtain, widely accessible and undetected on routine drug screening; furthermore, confirmed dextromethorphan dependence is rarely reported in the literature (Bryner et al, 2006;Desai et al, 2006;Navarro, Perry, & Bobo, 2006;Shin, Lee, Kim, Nabeshima, & Kim, 2008;Logan, Goldfogel, Hamilton, & Kuhlman, 2009). Metabolomics is a newly emerging omics approach to the investigation of metabolic phenotype changes induced by environmental or endogenous factors (Patti, Yanes, & Siuzdak, 2012;Deng et al, 2015aDeng et al, , 2015bWang et al, 2015aWang et al, , 2015bWen et al, 2015Wen et al, , 2016aWen et al, , 2016b.…”

Section: Changes In Metabolitementioning

confidence: 99%

Serum metabolic changes in rats after intragastric administration of dextromethorphan

Bao

Zhang

Zou

et al. 2016

Biomedical Chromatography

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Dextromethorphan is recognized as a substance of abuse around the world. An estimated 3.1 million people between the ages of 12 and 25 years (5.3%) misused over-the-counter cough and cold medications in 2006. In this study, we developed a serum metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of abuse of dextromethorphan on rats. The dextromethorphan-treated rats were given 12, 24 and 48 mg/kg (low, medium, high) of dextromethorphan by intragastric administration each day for 3 days. Partial least squares-discriminate analysis revealed that intragastric administration of dextromethorphan induced metabolic perturbations. Compared with the control (healthy) group, the levels of propanoic acid, urea, heptafluorobutanoic acid, 2-hexyldecanoic acid and butanedioic acid of the low group decreased; levels of propanoic acid and heptafluorobutanoic acid of the medium group decreased, while that of benzoic acid increased; and levels of 2-hexyldecanoic acid, glycerol and butanedioic acid of the high group increased. These biomarkers are involved in the citric acid cycle, urea cycle, glycerolipid metabolism and tricarboxylic acid cycle. The results indicate that the metabolomic method by GC-MS may be useful to elucidate abuse of dextromethorphan. According to the pathological changes in the liver at different dosages, dextromethorphan is not hepatotoxic after intragastric administration of 12, 24 and 48 mg/kg for 3 days.

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A gas chromatography–mass spectrometry based study on serum metabolomics in rats chronically poisoned with hydrogen sulfide

Cited by 21 publications

References 29 publications

Effect of alprazolam on rat serum metabolic profiles

Effect of alprazolam on rat serum metabolic profiles

Evaluation of the Role of Toxicological Data in Discriminating Between H₂S Femoral Blood Concentration Secondary to Lethal poisoning and Endogenous H₂S Putrefactive Production

Serum metabolic changes in rats after intragastric administration of dextromethorphan

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A gas chromatography–mass spectrometry based study on serum metabolomics in rats chronically poisoned with hydrogen sulfide

Cited by 21 publications

References 29 publications

Effect of alprazolam on rat serum metabolic profiles

Effect of alprazolam on rat serum metabolic profiles

Evaluation of the Role of Toxicological Data in Discriminating Between H2S Femoral Blood Concentration Secondary to Lethal poisoning and Endogenous H2S Putrefactive Production

Serum metabolic changes in rats after intragastric administration of dextromethorphan

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Evaluation of the Role of Toxicological Data in Discriminating Between H₂S Femoral Blood Concentration Secondary to Lethal poisoning and Endogenous H₂S Putrefactive Production