A gene controlling the early development of protoperithecium in Neurospora crassa

Tee, Tan Sai; Choke, Ho Coy

doi:10.1007/bf00333631

Cited by 28 publications

(3 citation statements)

References 12 publications

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“…Johnson [10] isolated a large number of mutants affected in female development and ordered these mutants based on the size of the developing protoperithecia. Vigfusson and Weiljer [28] , Tan and Ho [29] , and Mylyk and Thelkeld [30] also isolated female sterile mutants. Some of the mutations were mapped onto the N. crassa genetic map, while others were unmapped.…”

Section: Introductionmentioning

confidence: 97%

Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Chinnici

Caccamise

et al. 2014

PLoS ONE

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Using a screening protocol we have identified 68 genes that are required for female development in the filamentous fungus Neurospora crassa. We find that we can divide these genes into five general groups: 1) Genes encoding components of the PACC signal transduction pathway, 2) Other signal transduction pathway genes, including genes from the three N. crassa MAP kinase pathways, 3) Transcriptional factor genes, 4) Autophagy genes, and 5) Other miscellaneous genes. Complementation and RIP studies verified that these genes are needed for the formation of the female mating structure, the protoperithecium, and for the maturation of a fertilized protoperithecium into a perithecium. Perithecia grafting experiments demonstrate that the autophagy genes and the cell-to-cell fusion genes (the MAK-1 and MAK-2 pathway genes) are needed for the mobilization and movement of nutrients from an established vegetative hyphal network into the developing protoperithecium. Deletion mutants for the PACC pathway genes palA, palB, palC, palF, palH, and pacC were found to be defective in two aspects of female development. First, they were unable to initiate female development on synthetic crossing medium. However, they could form protoperithecia when grown on cellophane, on corn meal agar, or in response to the presence of nearby perithecia. Second, fertilized perithecia from PACC pathway mutants were unable to produce asci and complete female development. Protein localization experiments with a GFP-tagged PALA construct showed that PALA was localized in a peripheral punctate pattern, consistent with a signaling center associated with the ESCRT complex. The N. crassa PACC signal transduction pathway appears to be similar to the PacC/Rim101 pathway previously characterized in Aspergillus nidulans and Saccharomyces cerevisiae. In N. crassa the pathway plays a key role in regulating female development.

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Section: Introductionmentioning

confidence: 97%

Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Chinnici

Caccamise

et al. 2014

PLoS ONE

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“…Another ultraviolet-sensitive mutant, uvs-2, which appears to be cytoplasmic in nature, fails to form protoperithecia, although it functions well as the "male" parent (290). A protoperithecia-negative mutant, ff-1, has been isolated; it seems to result from a nuclear mutation (325). The ultraviolet sensitivity of the strain has not been tested.…”

Section: Turnover Of Rna and Protein During Meiosismentioning

confidence: 99%

Meiosis in protists. Some structural and physiological aspects of meiosis in algae, fungi, and protozoa.

Heywood¹,

Magee²

1976

Bacteriological Reviews

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“…The development of the fruiting structure in Neurospora has been the subject of biochemical and genetic investigation (1,8,10,29,31). A surprising amount of the detail of protoperithecial and perithecial development in N. tetrasperma was elucidated by the work of Dodge (8) and Backus (1) with the light microscope.…”

Section: Figmentioning

confidence: 99%

Life Cycle of Neurospora crassa Viewed by Scanning Electron Microscopy

Seale

1973

J Bacteriol

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Scanning electron microscopy was used to examine the major stages of the life cycle of two wild-type strains of Neurospora crassa Shear and Dodge (St. Lawrence 3.la and 74A): mycelia, protoperithecium formation, perithecia, ascospores, ascospore germination and outgrowth, macro and microconidia, and germination and outgrowth of macroconidia. Structures seen at the limit of resolution of bright-field and phase-contrast microscopes, e.g., the ribbed surface of ascospores, are well resolved. New details of conidial development and surface structure are revealed. There appears to be only one distinguishable morphological difference between the two strains. The pattern of germination and outgrowth which seems relatively constant for strain 74A or strain 3.1a, appears to be different for each. Conidia from strain 3.1a almost always germinate from a site between interconidial attachment points; whereas the germ tubes of strain 74A usually emerge from or very near the interconidial attachment site. These germination patterns usually do not segregate 2:2 in asci dissected in order. This observation suggests that conidial germination pattem is not under the control of a single gene.

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A gene controlling the early development of protoperithecium in Neurospora crassa

Cited by 28 publications

References 12 publications

Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Neurospora crassa Female Development Requires the PACC and Other Signal Transduction Pathways, Transcription Factors, Chromatin Remodeling, Cell-To-Cell Fusion, and Autophagy

Meiosis in protists. Some structural and physiological aspects of meiosis in algae, fungi, and protozoa.

Life Cycle of Neurospora crassa Viewed by Scanning Electron Microscopy

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