A general controllable release amplification strategy of liposomes for single-particle collision electrochemical biosensing

Magnetic Rolling Circle Amplification-Assisted Single-Particle Collision Immunosensor for Ultrasensitive Detection of Cardiac Troponin I

“…Under optimal conditions, collision frequency increased rapidly with cTnI concentrations at first and then slowly, reaching a platform (Figure A). Possible reasons were the binding constant between mAb and cTnI and the limited amount of mAb on the surface of MBs . Collision frequency had a good linear relationship with the logarithm of cTnI concentrations from 1 fg/mL to 50 ng/mL.…”

“…During collision experiments, the concentration of PBS was found to affect the stability of Pt NPs. , It is important to optimize the concentration of PBS to prevent Pt NPs aggregation. By comparing the hydration nanoparticle size of Pt NPs in various PBS concentrations (Figure A), the size remained stable in low concentrations of PBS but increased sharply when the concentration of PBS exceeded 10 mM.…”

“…Possible reasons were the binding constant between mAb and cTnI and the limited amount of mAb on the surface of MBs. 32 Collision frequency had a good linear relationship with the logarithm of cTnI concentrations from 1 fg/mL to 50 ng/mL. The linear equation was defined as f = 0.14 log c + 0.56 with a detection limit of 0.57 fg/mL (correlation coefficient R 2 = 0.9988, S/N = 3), which was more sensitive than other cTnI detection methods (Table S2).…”

Single-Nanoparticle Collision Electrochemistry Biosensor Based on an Electrocatalytic Strategy for Highly Sensitive and Specific Detection of H7N9 Avian Influenza Virus

“…Besides, the application of the electrocatalytic mode mainly relies on the indirect conversion of biological analytes into catalytic nanoparticles. , In this process, through the introduction of specific recognition of targets, such as antibodies and aptamers, the specificity of the method can be greatly improved. However, in present applications, the SNCE electrocatalysis mode is mainly used for the analysis of the basic properties of nanoparticles. ,, Recently, a few studies focusing on electrocatalysis and direct electrolysis of nanoparticles for bioanalytical detection have been reported. ,− ,, While the SNCE electrocatalytic method for bioanalytical detection has not been systematically developed, the detection sensitivity needs to be further improved. Therefore, it is necessary to develop a biosensor by taking a full advantage of the SNCE electrocatalytic mode and with the help of an efficient signal amplification strategy to achieve a highly sensitive and specific detection of biological analytes.…”

“…11,29,30 Recently, a few studies focusing on electrocatalysis and direct electrolysis of nanoparticles for bioanalytical detection have been reported. 8,[21][22][23]31,32 While the SNCE electrocatalytic method for bioanalytical detection has not been systematically developed, the detection sensitivity needs to be further improved. Therefore, it is necessary to develop a biosensor by taking a full advantage of the SNCE electrocatalytic mode and with the help of an efficient signal amplification strategy to achieve a highly sensitive and specific detection of biological analytes.…”