A General Use of Amberlite XAD-2 Resin for the Purification of Flavonoids from Aqueous Fractions

Rosler, Karl-Heinz A.; Goodwin, Robert S.

doi:10.1021/np50031a036

Cited by 22 publications

(8 citation statements)

References 1 publication

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“…The main problem in HPLC analysis of flavonoid from jams is the large amounts of sugar and pectins they contain, which make rather difficult flavonoid extraction and preparation of samples for analysis. The use of the resin Amberlite XAD-2 for the removal of sugars and polar compounds from plant extracts, to obtain a purified phenolic compound fraction, has been reported (Rosier and Goodwin, 1984). This technique has recently been used with success in the analysis of flavonoids from honey (Ferreres et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

Phenolic compounds analysis in the determination of fruit jam genuineness

Tomás‐Lorente¹,

García‐Viguera²,

Ferreres³

et al. 1992

J. Agric. Food Chem.

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Section: Resultsmentioning

confidence: 99%

Phenolic compounds analysis in the determination of fruit jam genuineness

Tomás‐Lorente¹,

García‐Viguera²,

Ferreres³

et al. 1992

J. Agric. Food Chem.

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“…Dried leaves (36 g) of each of susceptible 'Davis' and resistant PI 227687 soya bean genotypes were extracted in boiling 40% aqueous ethanol (EtOH) to carry out the first bioassay. After reducing to dryness in a rotary evaporator, the extract was redissolved in warm distilled water and filtered through an Amberlite XAD-2 resin (Rosler & Goodwin, 1983) to elute the bulk of water-soluble contaminants. Compounds adsorbed on the resin were eluted on 750 ml of each 50% aqueous methanol and metanol (MeOH), collected and reduced to dryness.…”

Section: Methodsmentioning

confidence: 99%

Pre‐ingestive and post‐ingestive effects of soya bean extracts and rutin on Trichoplusia ni growth

Hoffmann-Campo

Harborne

McCaffery

2001

Entomologia Exp Applicata

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Total ethanolic crude extracts from soya bean (Glycine max L. Merrill) PI 227687 and ‘Davis’, three purified fractions (A, B and C) from PI 227687, and pure rutin were tested for their effect on the growth of Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) larvae, from egg hatching to pupation. Analysis of covariance (ANCOVA), followed by bicoordinate utilisation plots, was used to remove the effect of feeding time from consumption and weight of pupae and to separate pre‐ ingestive and post‐ ingestive effects of different diets on T. ni growth. Survival, pupal weight, and feeding time of T. ni were all negatively affected by PI 227687 total extracts. Pupal weight and consumption remained affected by diet, after removing the effect of feeding time by ANCOVA. Larvae fed on the ‘Davis’ extract diet grew larger and consumed more rapidly a larger amount of food than those fed on PI 227687 and control diets. T. ni pupal weight was strongly influenced by consumption. In general, those insects that consumed more food were heavier but larvae fed on diet containing PI 227687 extract grew less than the others, irrespective of the amount of ingested and digested food. The feeding time was adversely affected by PI 227687 fraction A, composed of two flavonol glycosides (rutin and quercetin 3‐O‐glucosylgalactoside) and of an isoflavone glycoside (genistin). Survival and pupal weight were not affected, although the consumption and assimilation of ingested food was lower when larvae were fed on fractions A and C. The toxicity of the higher rutin concentration (2%) was similar to that observed when the insects were fed on unfractioned PI 227687, except that effects on food consumption were not observed. Results indicated that the feeding deterrence of PI 227687 must be caused by other compounds from fractions A and C. Moreover, rutin, acting as an antibiotic, appears to account for the adverse effects of PI 227687 on the physiology of T. ni.

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“…In the last ten years, several non-ionic polymeric resins (Amberlite XAD) have been used for the recovery of flavonoids from plant extracts with the elimination of the water-soluble contaminants. Thus, the polystyrene resin Amberlite XAD-2 was used by Rosler and Goodwin (1984) and Maggi ef al. (1989), and the polyacrylic resins XAD-7 and XAD-8 by Markham (1989) and Maggi et al (1989).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of different amberlite XAD resins in flavonoid analysis

Tomás-Barberán

Blázquez

Garcı́a-Viguera

et al. 1992

Phytochemical Analysis

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A comparative study of the recovery of flavonoids (both aglycones and glycosides) by different Amberlite XAD resins has been achieved. No significant differences in the percentages of recovery were found with the chemical nature and physical characteristics of the different resins. A flavonoid recovery higher than 90% is possible in a number of cases. The possibility of fractionating the adsorbed flavonoids by desorption with methanol: water mixtures has also been studied. The polystyrene resins (XAD‐2, XAD‐4 and XAD‐16) were found to be more useful for fractionation purposes than the polyacrylic resins (XAD‐7, XAD‐8). The results suggest that Amberlite XAD‐2 is the most suitable for the recovery and fractionation of flavonoids from plant extracts and foods rich in sugars and other polar compounds such as honey and fruit jams.

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A General Use of Amberlite XAD-2 Resin for the Purification of Flavonoids from Aqueous Fractions

Cited by 22 publications

References 1 publication

Phenolic compounds analysis in the determination of fruit jam genuineness

Phenolic compounds analysis in the determination of fruit jam genuineness

Pre‐ingestive and post‐ingestive effects of soya bean extracts and rutin on Trichoplusia ni growth

A comparative study of different amberlite XAD resins in flavonoid analysis

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